| IED ID | IndEnz0002015663 |
| Enzyme Type ID | protease015663 |
| Protein Name |
Non-structural polyprotein 1AB Cleaved into: VPg; Protein p19; Transmembrane protein 1A; Serine protease p27 p27 EC 3.4.21.- ; Protein p20; RNA-directed RNA polymerase p57 p57 EC 2.7.7.48 |
| Gene Name | ORF1 |
| Organism | Human astrovirus-4 (HAstV-4) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Stelpaviricetes Stellavirales Astroviridae Mamastrovirus Mamastrovirus 1 Human astrovirus-4 (HAstV-4) |
| Enzyme Sequence | MAHGEPYYSSKPDKDFNFGSTMARRQMTPTMVAKLPNFVRNSPQAYDWIVRGLIFPTTGKTYFQRVVVITGGLEDGTYGSFVFDGREWVEIYPIEHLNLMSSLKLIHKANALQERLRLSQEEKATLALDVQFLQHENVRLKELIPKPEPRKIQMKWIIVGAVLTFLSLIPGGYAQSQINNTIFTDMIAACKYSTETLTENLDLRIKLALANITISDKLDAVRQILNFAFVPRAHWLRTVFYYIHYYEMWNIFMFVLAIGTVMRSARPGTDLITLATSHLSGFRMAVLPTIPFHTTMTLWVMNTLMVCYYFDNLLAITLAILAPILGIIFLCFMEDSNYVSQIRGLIATAVLIAGGHACLTLTGTTTSLFVVILTCRFIRMATVFIGTRFEIRDANGKVVATVPTRIKNVAFDFFQKLKQSGVRVGVNEFVVIKPGALCVIDTPEGKGTGFFSGNDIVTAAHAVGNNTFVNVCYEGLMYEAKVRYMPEKDIAFITCPGDLHPTARLKLSKNPDYSCVTVMAYVNEDLVVSTAAAMVHGNTLSYAVRTQDGMSGAPVCDKYGRVLAVHQTNTGYTGGAVIIDPTDFHPVKAPSRVELLKEEIERLKAQLNSAAENPATAVTQQPVVTLEQKSVSDSDVVDLVRTAMEREMKVLRDEINGILAPFLQKKKGKTKHGRGRVRRNLRKGVKLLTEEEYRELLEKGLDRETFLDLIDRIIGERSGYPDYDDEDYYDEDDDGWGVVGDDVEFDYTEVINFDQAKPTPAPRTVKPKTCPEPEAETQPLDLSQKKEKQLEHEQQVVKSTKPQKNEPQPYSQTYGKAPIWESYDFDWDEDDAKFILPAPHRLTKADEIVLGSKIVKLRTIIETAIKTQNYSALPEAVFELDKAAYEAGLEGFLQRVKSKKGSKKLQRAPEDQGAQNYHSLDAWKSLLEPPRERRCVPANFPLLGHLPINRPIFDDKKPRDDLLGLLPEPTWHAFEEYGPTTWGPQAFIKSFDKFFYAEPIDFFSEYPQLCAFADWATYREFRYLEDTRVIHITATEKNTDSTPAYPKMNYFDTEENYLEAHGWAPYIREFTRVFKGDKPEVLWYLFLKKEIIKEEKIRNSDIRQIVCADPIYTRIGACLEAHQNALMKQHTDTSVGQCGWSPMEGGFKKTMQRLVNKGNKHFIEFDWTRYDGTIPPALFKHIKEIRWNFINKDQREKYKHVHEWYVDNLLNRHVLLPSGEVTLQTRGNPSGQFSTTMDNNMVNFWSQAFEFAYFNGPDKDLWKTYDTVVYGDDRLSTTPSVPDDYEERVINMYRDIFGMWVKPGKVICRDSIVGLSFCGFTVNENLEPVPTSPEKLMASLLKPYKILPDLESLHGKLLCYQLLAAFMAEDHPFKVYVEHCLSRTAKQLRDSGLPARLTEEQLHRIWRGGPKKCDG |
| Enzyme Length | 1415 |
| Uniprot Accession Number | Q3ZN06 |
| Absorption | |
| Active Site | ACT_SITE 461; /note=Charge relay system; for serine protease activity; /evidence=ECO:0000250; ACT_SITE 489; /note=Charge relay system; for serine protease activity; /evidence=ECO:0000250; ACT_SITE 551; /note=Charge relay system; for serine protease activity; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; |
| DNA Binding | |
| EC Number | 3.4.21.-; 2.7.7.48 |
| Enzyme Function | FUNCTION: [Non-structural polyprotein 1AB]: Contains the viral protease participating in the cleavage of the polyprotein into functional products. It contains also the activities necessary for replication of genomic RNA, as well as transcription of subgenomic mRNA (By similarity). {ECO:0000250}.; FUNCTION: [VPg]: Protein covalently attached to the 5' extremity of the genomic and subgenomic RNAs. {ECO:0000305|PubMed:22787221}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (7); Coiled coil (2); Compositional bias (1); Domain (1); Region (1); Site (5); Transmembrane (5) |
| Keywords | ATP-binding;Coiled coil;Direct protein sequencing;Host membrane;Hydrolase;Membrane;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Ribosomal frameshifting;Thiol protease;Transferase;Transmembrane;Transmembrane helix;Viral RNA replication |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: Cleaved presumably by viral and host proteases. {ECO:0000250}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 161,278 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21248 |
| Cross Reference Brenda |