| IED ID | IndEnz0002015672 |
| Enzyme Type ID | protease015672 |
| Protein Name |
Non-structural polyprotein 1A Cleaved into: VPg; Protein p19; Transmembrane protein 1A; Serine protease p27 p27 EC 3.4.21.- ; Protein p20' |
| Gene Name | ORF1 |
| Organism | Human astrovirus-4 (HAstV-4) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Stelpaviricetes Stellavirales Astroviridae Mamastrovirus Mamastrovirus 1 Human astrovirus-4 (HAstV-4) |
| Enzyme Sequence | MAHGEPYYSSKPDKDFNFGSTMARRQMTPTMVAKLPNFVRNSPQAYDWIVRGLIFPTTGKTYFQRVVVITGGLEDGTYGSFVFDGREWVEIYPIEHLNLMSSLKLIHKANALQERLRLSQEEKATLALDVQFLQHENVRLKELIPKPEPRKIQMKWIIVGAVLTFLSLIPGGYAQSQINNTIFTDMIAACKYSTETLTENLDLRIKLALANITISDKLDAVRQILNFAFVPRAHWLRTVFYYIHYYEMWNIFMFVLAIGTVMRSARPGTDLITLATSHLSGFRMAVLPTIPFHTTMTLWVMNTLMVCYYFDNLLAITLAILAPILGIIFLCFMEDSNYVSQIRGLIATAVLIAGGHACLTLTGTTTSLFVVILTCRFIRMATVFIGTRFEIRDANGKVVATVPTRIKNVAFDFFQKLKQSGVRVGVNEFVVIKPGALCVIDTPEGKGTGFFSGNDIVTAAHAVGNNTFVNVCYEGLMYEAKVRYMPEKDIAFITCPGDLHPTARLKLSKNPDYSCVTVMAYVNEDLVVSTAAAMVHGNTLSYAVRTQDGMSGAPVCDKYGRVLAVHQTNTGYTGGAVIIDPTDFHPVKAPSRVELLKEEIERLKAQLNSAAENPATAVTQQPVVTLEQKSVSDSDVVDLVRTAMEREMKVLRDEINGILAPFLQKKKGKTKHGRGRVRRNLRKGVKLLTEEEYRELLEKGLDRETFLDLIDRIIGERSGYPDYDDEDYYDEDDDGWGVVGDDVEFDYTEVINFDQAKPTPAPRTVKPKTCPEPEAETQPLDLSQKKEKQLEHEQQVVKSTKPQKNEPQPYSQTYGKAPIWESYDFDWDEDDAKFILPAPHRLTKADEIVLGSKIVKLRTIIETAIKTQNYSALPEAVFELDKAAYEAGLEGFLQRVKSKNKAPKNYKGPQKTKGPKTITH |
| Enzyme Length | 920 |
| Uniprot Accession Number | Q3ZN07 |
| Absorption | |
| Active Site | ACT_SITE 461; /note=Charge relay system; for serine protease activity; /evidence=ECO:0000250; ACT_SITE 489; /note=Charge relay system; for serine protease activity; /evidence=ECO:0000250; ACT_SITE 551; /note=Charge relay system; for serine protease activity; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: [Non-structural polyprotein 1A]: Contains the viral protease participating in the cleavage of the polyprotein into functional products. {ECO:0000269|PubMed:22787221}.; FUNCTION: [VPg]: Protein covalently attached to the 5' extremity of the genomic and subgenomic RNAs. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (6); Compositional bias (1); Region (2); Site (4); Transmembrane (4) |
| Keywords | Coiled coil;Direct protein sequencing;Host membrane;Hydrolase;Membrane;Protease;Ribosomal frameshifting;Thiol protease;Transmembrane;Transmembrane helix;Viral RNA replication |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: Cleaved presumably by viral and host proteases. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 103,386 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21248 |
| Cross Reference Brenda |