| IED ID | IndEnz0002015702 |
| Enzyme Type ID | protease015702 |
| Protein Name |
ATP-dependent protease ATPase subunit HslU Unfoldase HslU |
| Gene Name | hslU HDEF_1270 |
| Organism | Hamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Enterobacteriaceae incertae sedis ant tsetse mealybug aphid etc. endosymbionts aphid secondary symbionts Candidatus Hamiltonella Candidatus Hamiltonella defensa Hamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT) |
| Enzyme Sequence | MSDMTPSEIVKKLDDYIIGQDDAKRAVAIALRNRWRRMQLDTELRQEITPKNILMIGPTGVGKTEIARRLAKLAEAPFIKVEATKFTEVGYVGKEVESIIRDLTDTAVKMVRHQAIEKMRDKAKELAQERILDILVSPARNSSGQVEGSQNTSTARQFFLEPLIKGELNEQEIEIDLAVTPVDIEIMSPPGMEEMTNQLQSMFQHMSSQKHKIRKMKIGDALKLLIDEEASKLVNPEELKQVAIHSVEQNGIVFIDEVDKICRHGSKSTSDVSREGVQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQVAKPSDLIPELQGRFPIRVELKSLTPKDFERILTEPSASLTYQYKSLMKTEGIKIEFTKDGITSIAGAAGQVNENTENIGARRLHTILERVMEDISYNADKIKKKSITIDSKYVDTHLSKLINNEDLSRFIL |
| Enzyme Length | 443 |
| Uniprot Accession Number | C4K5T0 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 18; /note=ATP; via amide nitrogen and carbonyl oxygen; /evidence=ECO:0000255|HAMAP-Rule:MF_00249; BINDING 256; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_00249; BINDING 321; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_00249; BINDING 393; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_00249 |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis. {ECO:0000255|HAMAP-Rule:MF_00249}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 60..65; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_00249 |
| Features | Binding site (4); Chain (1); Nucleotide binding (1) |
| Keywords | ATP-binding;Chaperone;Cytoplasm;Nucleotide-binding;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00249}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 49,792 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |