| IED ID | IndEnz0002015745 |
| Enzyme Type ID | protease015745 |
| Protein Name |
Kexin EC 3.4.21.61 Protease KEX2 Proteinase YSCF |
| Gene Name | KEX2 QDS1 YNL238W N1122 |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MKVRKYITLCFWWAFSTSALVSSQQIPLKDHTSRQYFAVESNETLSRLEEMHPNWKYEHDVRGLPNHYVFSKELLKLGKRSSLEELQGDNNDHILSVHDLFPRNDLFKRLPVPAPPMDSSLLPVKEAEDKLSINDPLFERQWHLVNPSFPGSDINVLDLWYNNITGAGVVAAIVDDGLDYENEDLKDNFCAEGSWDFNDNTNLPKPRLSDDYHGTRCAGEIAAKKGNNFCGVGVGYNAKISGIRILSGDITTEDEAASLIYGLDVNDIYSCSWGPADDGRHLQGPSDLVKKALVKGVTEGRDSKGAIYVFASGNGGTRGDNCNYDGYTNSIYSITIGAIDHKDLHPPYSEGCSAVMAVTYSSGSGEYIHSSDINGRCSNSHGGTSAAAPLAAGVYTLLLEANPNLTWRDVQYLSILSAVGLEKNADGDWRDSAMGKKYSHRYGFGKIDAHKLIEMSKTWENVNAQTWFYLPTLYVSQSTNSTEETLESVITISEKSLQDANFKRIEHVTVTVDIDTEIRGTTTVDLISPAGIISNLGVVRPRDVSSEGFKDWTFMSVAHWGENGVGDWKIKVKTTENGHRIDFHSWRLKLFGESIDSSKTETFVFGNDKEEVEPAATESTVSQYSASSTSISISATSTSSISIGVETSAIPQTTTASTDPDSDPNTPKKLSSPRQAMHYFLTIFLIGATFLVLYFMFFMKSRRRIRRSRAETYEFDIIDTDSEYDSTLDNGTSGITEPEEVEDFDFDLSDEDHLASLSSSENGDAEHTIDSVLTNENPFSDPIKQKFPNDANAESASNKLQELQPDVPPSSGRS |
| Enzyme Length | 814 |
| Uniprot Accession Number | P13134 |
| Absorption | |
| Active Site | ACT_SITE 175; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 213; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 385; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleavage of -Lys-Arg-|-Xaa- and -Arg-Arg-|-Xaa- bonds to process yeast alpha-factor pheromone and killer toxin precursors.; EC=3.4.21.61; |
| DNA Binding | |
| EC Number | 3.4.21.61 |
| Enzyme Function | FUNCTION: Processing of precursors of alpha-factors and killer toxin. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (22); Chain (1); Compositional bias (2); Disulfide bond (2); Domain (2); Glycosylation (4); Helix (16); Metal binding (6); Propeptide (2); Region (2); Signal peptide (1); Topological domain (2); Transmembrane (1); Turn (9) |
| Keywords | 3D-structure;Calcium;Cleavage on pair of basic residues;Disulfide bond;Glycoprotein;Golgi apparatus;Hydrolase;Membrane;Metal-binding;Protease;Reference proteome;Serine protease;Signal;Transmembrane;Transmembrane helix;Zymogen |
| Interact With | P32790 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. |
| Modified Residue | |
| Post Translational Modification | PTM: O-glycosylated. |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (3) |
| Cross Reference PDB | 1OT5; 1R64; 2ID4; |
| Mapped Pubmed ID | 10393104; 10409610; 10438739; 10564262; 10567340; 10704473; 10848621; 10918038; 11092829; 11160819; 11284720; 11297433; 11728446; 11805837; 11893737; 11995965; 12221112; 12244127; 12827498; 12832612; 1367896; 1368916; 1444259; 14528262; 1483466; 1493334; 14992578; 14999401; 15090613; 15102434; 15140896; 15299026; 15364946; 1543492; 15647379; 15710404; 1574114; 15913810; 16029153; 16167351; 1618297; 16229820; 16429126; 16554755; 16913836; 17210951; 17363896; 1740121; 17426142; 17630978; 17975704; 18415848; 1843278; 1843279; 18467557; 18625069; 18784256; 18979235; 18987790; 18998772; 19536198; 19688096; 19726565; 1988035; 20150508; 2022624; 20232931; 20953094; 2099807; 21252230; 21377728; 21496492; 2165411; 2193026; 21987636; 22396539; 22533807; 22679642; 22933563; 23408788; 23498901; 25886139; 26070720; 26344761; 2647083; 26709839; 2683070; 26849222; 27693354; 27720750; 27965112; 27977123; 2902090; 3059713; 32800900; 3288097; 3297783; 6353202; 6397123; 6430565; 7819327; 7845204; 7957171; 8162413; 8194519; 8224490; 8308064; 8328974; 8420571; 8488726; 8495725; 8509444; 8887651; 8917107; 8930891; 8987562; 8991091; 9070434; 9314526; 9360297; 9417119; 9724712; |
| Motif | |
| Gene Encoded By | |
| Mass | 90,003 |
| Kinetics | |
| Metal Binding | METAL 135; /note=Calcium 1; METAL 184; /note=Calcium 1; METAL 227; /note=Calcium 1; METAL 277; /note=Calcium 2; METAL 320; /note=Calcium 2; METAL 350; /note=Calcium 2 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.61; |