| IED ID | IndEnz0002015754 |
| Enzyme Type ID | protease015754 |
| Protein Name |
Kallikrein-1E2 EC 3.4.21.35 Glandular kallikrein HPK |
| Gene Name | KLK1E2 |
| Organism | Equus caballus (Horse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Perissodactyla (odd-toed ungulates) Equidae (horses) Equus Equus Equus caballus (Horse) |
| Enzyme Sequence | MWFLVLCLDLSLGETGALPPIQSRIIGGWECEKHSKPWQVAVYHQGHFQCGGVLVHPQWVLTAAHCMSDDYQIWLGRHNLSEDEDTAQFHQVSDSFLDPQFDLSLLKKKYLRPYDDISHDLMLLRLAQPARITDAVKILDLPTQEPKLGSTCYTSGWGLISTFTNRGSGTLQCVELRLQSNEKCARAYPEKMTEFVLCATHRDDSGSICLGDSGGALICDGVFQGITSWGYSECADFNDNFVFTKVMPHLKWIKETIEKNS |
| Enzyme Length | 261 |
| Uniprot Accession Number | Q6H321 |
| Absorption | |
| Active Site | ACT_SITE 65; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 120; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 213; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.; EC=3.4.21.35; |
| DNA Binding | |
| EC Number | 3.4.21.35 |
| Enzyme Function | FUNCTION: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (14); Chain (1); Disulfide bond (5); Domain (1); Glycosylation (1); Helix (7); Propeptide (1); Sequence conflict (2); Signal peptide (1); Turn (2) |
| Keywords | 3D-structure;Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:12217694}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..17; /evidence=ECO:0000305 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 1GVZ; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 29,392 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |