| IED ID | IndEnz0002015880 |
| Enzyme Type ID | protease015880 |
| Protein Name |
Prophage integrase IntA Prophage CP4-57 integrase SlpA |
| Gene Name | intA intX slpA b2622 JW2602 |
| Organism | Escherichia coli (strain K12) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) |
| Enzyme Sequence | MARKTKPLTDTEIKAAKPKDADYQLYDGDGLTLLIKSSGSKLWQFRYYRPLTKQRTKQSFGAYPAVSLSDARKLRAESKVLLAKDIDPQEHQKEQVRNSQEAKTNTFLLVAERWWNVKKTSVTEDYADDIWRSLERDIFPAIGDISITEIKAHTLVKAVQPVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQLPQLMHTMRTASISMSTRCLFMWQLLTITRPAEAAEARWDEIDFNASEWKIPAARMKMNRDHTVPLSDGALAILEMMKPLSGGREFIFPSRIKPNQPMNSQTVNAALKRAGLGGVLVSHGLRSIASTALNEEGFPPDVIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWADLVKAADSGSIVLTHLSKIRLVG |
| Enzyme Length | 413 |
| Uniprot Accession Number | P32053 |
| Absorption | |
| Active Site | ACT_SITE 248; /evidence=ECO:0000255|PROSITE-ProRule:PRU01246; ACT_SITE 275; /evidence=ECO:0000255|PROSITE-ProRule:PRU01246; ACT_SITE 337; /evidence=ECO:0000255|PROSITE-ProRule:PRU01246; ACT_SITE 340; /evidence=ECO:0000255|PROSITE-ProRule:PRU01246; ACT_SITE 363; /evidence=ECO:0000255|PROSITE-ProRule:PRU01246; ACT_SITE 373; /note=O-(3'-phospho-DNA)-tyrosine intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU01246 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome. Part of the cryptic P4-like prophage CP4-57, it excises the prophage when overexpressed, which also requires integration host factor (encoded by ihfA and ihfB) (PubMed:7511583). Overexpression of AlpA leads to excision of the CP4-57 prophage, which inactivates ssrA (the gene upstream of the prophage) that encodes tmRNA which is required to rescue stalled ribosomes in a process known as trans-translation (PubMed:7511582). {ECO:0000269|PubMed:7511583}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (6); Chain (1); Domain (2) |
| Keywords | DNA integration;DNA recombination;DNA-binding;Reference proteome;Viral genome integration;Virus entry into host cell |
| Interact With | P06959; P06864 |
| Induction | INDUCTION: By AlpA (PubMed:7511582). {ECO:0000269|PubMed:7511582}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15690043; 16606699; 24561554; |
| Motif | |
| Gene Encoded By | |
| Mass | 46,652 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |