| IED ID | IndEnz0002015882 |
| Enzyme Type ID | protease015882 |
| Protein Name |
Mast/stem cell growth factor receptor Kit SCFR EC 2.7.10.1 Proto-oncogene c-Kit Tyrosine-protein kinase Kit |
| Gene Name | KIT |
| Organism | Gallus gallus (Chicken) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Archelosauria Archosauria Dinosauria Saurischia Theropoda Coelurosauria Aves Neognathae Galloanserae Galliformes Phasianidae (turkeys) Phasianinae Gallus Gallus gallus (Chicken) |
| Enzyme Sequence | MEGAHLAWELAHAVLLLSLIPAGGSVPHEESSLVVNKGEELRLKCNEEGPVTWNFQNSDPSAKTRISNEKEWHTKNATIRDIGRYECKSKGSIVNSFYVFVKDPNVLFLVDSLIYGKEDSDILLVCPLTDPDVLNFTLRKCDGKPLPKNMTFIPNPQKGIIIKNVQRSFKGCYQCLAKHNGVEKISEHIFLNVRPVHKALPVITLSKSYELLKEGEEFEVTCIITDVDSSVKASWISYKSAIVTSKSRNLGDYGYERKLTLNIRSVGVNDSGEFTCQAENPFGKTNATVTLKALAKGFVRLFATMNTTIDINAGQNGNLTVEYEAYPKPKEEVWMYMNETLQNSSDHYVKFKTVGNNSYTSELHLTRLKGTEGGIYTFFVSNSDASSSVTFNVYVKTKPEILTLDMLGNDILQCVATGFPAPTIYWYFCPGTEQRCLDSPTISPMDVKVSYTNSSVPSFERILVESTVNASMFKSTGTICCEASSNGDKSSVFFNFAIKEQIRTHTLFTPLLIAFGVAAGLMCIIVMILVYIYLQKPKYEVQWKVVEEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLFKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHINIVNLLGACTIGGPTLVITEYCCYGDLLNFLRRKRDSFICPKHEEHAEAAVYENLLHQAEPTADAVNEYMDMKPGVSYAVPPKADKKRPVKSGSYTDQDVTLSMLEDDELALDVEDLLSFSYQVAKGMSFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIRNDSNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGILLWELFSLGSSPYPGMPVDSKFYKMIKEGYRMFSPECSPPEMYDIMKSCWDADPLQRPTFKQIVQLIEQQLSDNAPRVYANFSTPPSTQGNATDHSVRINSVGSSASSTQPLLVREDV |
| Enzyme Length | 960 |
| Uniprot Accession Number | Q08156 |
| Absorption | |
| Active Site | ACT_SITE 777; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000255|PROSITE-ProRule:PRU10028" |
| Activity Regulation | |
| Binding Site | BINDING 609; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00159; BINDING 781; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00159 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-[protein]; Xref=Rhea:RHEA:10596, Rhea:RHEA-COMP:10136, Rhea:RHEA-COMP:10137, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:46858, ChEBI:CHEBI:82620, ChEBI:CHEBI:456216; EC=2.7.10.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10028}; |
| DNA Binding | |
| EC Number | 2.7.10.1 |
| Enzyme Function | FUNCTION: Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. KIT promotes activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 582..589; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00159; NP_BIND 657..663; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00159 |
| Features | Active site (1); Binding site (2); Chain (1); Disulfide bond (5); Domain (6); Glycosylation (12); Metal binding (3); Modified residue (6); Nucleotide binding (2); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | ATP-binding;Cell membrane;Disulfide bond;Glycoprotein;Immunoglobulin domain;Kinase;Magnesium;Membrane;Metal-binding;Nucleotide-binding;Phosphoprotein;Proto-oncogene;Receptor;Reference proteome;Repeat;Signal;Transferase;Transmembrane;Transmembrane helix;Tyrosine-protein kinase;Ubl conjugation |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. |
| Modified Residue | MOD_RES 554; /note=Phosphotyrosine; by autocatalysis; /evidence=ECO:0000250; MOD_RES 556; /note=Phosphotyrosine; by autocatalysis; /evidence=ECO:0000250; MOD_RES 689; /note=Phosphotyrosine; by autocatalysis; /evidence=ECO:0000250; MOD_RES 706; /note=Phosphotyrosine; by autocatalysis; /evidence=ECO:0000250; MOD_RES 808; /note=Phosphotyrosine; by autocatalysis; /evidence=ECO:0000250; MOD_RES 921; /note=Phosphotyrosine; by autocatalysis; /evidence=ECO:0000250 |
| Post Translational Modification | PTM: Ubiquitinated. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation. {ECO:0000250}.; PTM: Autophosphorylated on tyrosine residues. KITLG/SCF binding promotes autophosphorylation. Phosphorylated tyrosine residues are important for interaction with specific binding partners (By similarity). {ECO:0000250}. |
| Signal Peptide | SIGNAL 1..24; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 107,312 |
| Kinetics | |
| Metal Binding | METAL 554; /note=Magnesium; /evidence=ECO:0000250; METAL 782; /note=Magnesium; /evidence=ECO:0000250; METAL 795; /note=Magnesium; /evidence=ECO:0000250 |
| Rhea ID | RHEA:10596 |
| Cross Reference Brenda | 2.7.10.1; |