| IED ID | IndEnz0002016114 |
| Enzyme Type ID | protease016114 |
| Protein Name |
MPN domain-containing protein EC 3.4.-.- |
| Gene Name | mpnd zgc:153076 |
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio) |
| Enzyme Sequence | MGSEPPSSPQVVEEGADEEDEELSGAEDADLRSSSGRGSLLTRRGITLRVLLKDGLVEPGDGVLSIHYLGKKFVGDLLNDGKIRWVETGQIFNSPSAWATHCKRLVNPAKKSGCGWASVRYRGQKLVQYKTTWLHKYQPSADMSLISEGEDDEMGDDDEEEGKTTIPVEDKNKKSKPELHEIGLTQRRDRERIPVRYCTLGTRDAARDPHTLVELSAFSAINRFQPFNVAVSSNVLLLMDFHCHLTSSEVVGYLGGRWDTNTQLLTVLRAFPCRTRLADKDAAPAVEEEICQNLFMRGLSLVGWYHSHPRGPALPSLQDIDSQMDHQLRLQGSSNGFQPCLGIICGPYYHGNQGVASTITPFWVVPPPEQRPNDHGIPVAVEVTYVQDNFLTTDVLNEMMLLVEFYRSAPDLVQFSQMWSPNTSILDKIKASLSGHAPKDQAYAQILEHVYNQLRNTQ |
| Enzyme Length | 458 |
| Uniprot Accession Number | Q08CH3 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.-.- |
| Enzyme Function | FUNCTION: Probable protease (By similarity). Acts as a sensor of N(6)-methyladenosine methylation on DNA (m6A): recognizes and binds m6A DNA, leading to its degradation (By similarity). {ECO:0000250|UniProtKB:Q5VVJ2, ECO:0000250|UniProtKB:Q8N594}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (1); Domain (2); Metal binding (3); Motif (1); Region (2) |
| Keywords | Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Degraded following binding to N(6)-methyladenosine methylated DNA (m6A). {ECO:0000250|UniProtKB:Q8N594}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 20040115; |
| Motif | MOTIF 306..319; /note=JAMM motif; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182 |
| Gene Encoded By | |
| Mass | 51,102 |
| Kinetics | |
| Metal Binding | METAL 306; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182; METAL 308; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182; METAL 319; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182 |
| Rhea ID | |
| Cross Reference Brenda |