| IED ID | IndEnz0002016126 |
| Enzyme Type ID | protease016126 |
| Protein Name |
EEF1A lysine methyltransferase 4 EC 2.1.1.- |
| Gene Name | EEF1AKMT4 |
| Organism | Bos taurus (Bovine) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Ruminantia Pecora Bovidae Bovinae Bos (oxen cattle) Bos taurus (Bovine) |
| Enzyme Sequence | MACLGPSAQVPELPEKNCGYREVQYWDQRYQGAADSAPYEWFGDFSCFRDLLEPELRPLDRILVLGCGNSALSYEIFLGGFPDVTSVDYSSVVVAAMRARYAHVPTLRWETMDVRALGFPSGSFDVVLEKGTLDALLTGEQDPWTVSSEGVHTVDQVLNEVSRVLVPAGRFISLTSAAPHFRTRHYAQAHYGWSLRHATYGNGFQFHFYLMQKGKELSVAQLAVGAQILSPPRPPTPSCFLQDSDHEDFLSAIQL |
| Enzyme Length | 255 |
| Uniprot Accession Number | P0DPE1 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 26; /note=S-adenosyl-L-methionine; /evidence=ECO:0000250|UniProtKB:P0DPD7; BINDING 30; /note=S-adenosyl-L-methionine; /evidence=ECO:0000250|UniProtKB:P0DPD7; BINDING 41; /note=S-adenosyl-L-methionine; via amide nitrogen; /evidence=ECO:0000250|UniProtKB:P0DPD7; BINDING 66; /note=S-adenosyl-L-methionine; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P0DPD7; BINDING 130; /note=S-adenosyl-L-methionine; /evidence=ECO:0000250|UniProtKB:P0DPD7 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=L-lysyl-[protein] + S-adenosyl-L-methionine = H(+) + N(6)-methyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:51736, Rhea:RHEA-COMP:9752, Rhea:RHEA-COMP:13053, ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:61929; Evidence={ECO:0000250|UniProtKB:P0DPD7}; CATALYTIC ACTIVITY: Reaction=N(6)-methyl-L-lysyl-[protein] + S-adenosyl-L-methionine = H(+) + N(6),N(6)-dimethyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:54196, Rhea:RHEA-COMP:13053, Rhea:RHEA-COMP:13827, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:61929, ChEBI:CHEBI:61976; Evidence={ECO:0000250|UniProtKB:P0DPD7}; CATALYTIC ACTIVITY: Reaction=N(6),N(6)-dimethyl-L-lysyl-[protein] + S-adenosyl-L-methionine = H(+) + N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:54200, Rhea:RHEA-COMP:13826, Rhea:RHEA-COMP:13827, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:61961, ChEBI:CHEBI:61976; Evidence={ECO:0000250|UniProtKB:P0DPD7}; |
| DNA Binding | |
| EC Number | 2.1.1.- |
| Enzyme Function | FUNCTION: Protein-lysine methyltransferase that efficiently catalyzes three successive methylations on 'Lys-36' in eukaryotic translation elongation factor 1 alpha (EEF1A1 or EEF1A2). {ECO:0000250|UniProtKB:P0DPD7}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Binding site (5); Chain (1); Modified residue (1); Motif (1); Region (2); Sequence conflict (1) |
| Keywords | Alternative splicing;Methyltransferase;Phosphoprotein;Reference proteome;S-adenosyl-L-methionine;Transferase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | MOD_RES 39; /note=Phosphotyrosine; /evidence=ECO:0000250|UniProtKB:P0DPD7 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 25370848; |
| Motif | MOTIF 129..134; /note=Required for methyltransferase activity; /evidence=ECO:0000250|UniProtKB:P0DPD7 |
| Gene Encoded By | |
| Mass | 28,396 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:51736; RHEA:54196; RHEA:54200 |
| Cross Reference Brenda |