| IED ID | IndEnz0002016153 |
| Enzyme Type ID | protease016153 |
| Protein Name |
A disintegrin and metalloproteinase with thrombospondin motifs gon-1 ADAMTS gon-1 EC 3.4.24.- Abnormal gonad development protein 1 |
| Gene Name | gon-1 F25H8.3 |
| Organism | Caenorhabditis elegans |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans |
| Enzyme Sequence | MRSIGGSFHLLQPVVAALILLVVCLVYALQSGSGTISEFSSDVLFSRAKYSGVPVHHSRWRQDAGIHVIDSHHIVRRDSYGRRGKRDVTSTDRRRRLQGVARDCGHACHLRLRSDDAVYIVHLHRWNQIPDSHNKSVPHFSNSNFAPMVLYLDSEEEVRGGMSRTDPDCIYRAHVKGVHQHSIVNLCDSEDGLYGMLALPSGIHTVEPIISGNGTEHDGASRHRQHLVRKFDPMHFKSFDHLNSTSVNETETTVATWQDQWEDVIERKARSRRAANSWDHYVEVLVVADTKMYEYHGRSLEDYVLTLFSTVASIYRHQSLRASINVVVVKLIVLKTENAGPRITQNAQQTLQDFCRWQQYYNDPDDSSVQHHDVAILLTRKDICRSQGKCDTLGLAELGTMCDMQKSCAIIEDNGLSAAFTIAHELGHVFSIPHDDERKCSTYMPVNKNNFHIMAPTLEYNTHPWSWSPCSAGMLERFLENNRGQTQCLFDQPVERRYYEDVFVRDEPGKKYDAHQQCKFVFGPASELCPYMPTCRRLWCATFYGSQMGCRTQHMPWADGTPCDESRSMFCHHGACVRLAPESLTKIDGQWGDWRSWGECSRTCGGGVQKGLRDCDSPKPRNGGKYCVGQRERYRSCNTQECPWDTQPYREVQCSEFNNKDIGIQGVASTNTHWVPKYANVAPNERCKLYCRLSGSAAFYLLRDKVVDGTPCDRNGDDICVAGACMPAGCDHQLHSTLRRDKCGVCGGDDSSCKVVKGTFNEQGTFGYNEVMKIPAGSANIDIRQKGYNNMKEDDNYLSLRAANGEFLLNGHFQVSLARQQIAFQDTVLEYSGSDAIIERINGTGPIRSDIYVHVLSVGSHPPDISYEYMTAAVPNAVIRPISSALYLWRVTDTWTECDRACRGQQSQKLMCLDMSTHRQSHDRNCQNVLKPKQATRMCNIDCSTRWITEDVSSCSAKCGSGQKRQRVSCVKMEGDRQTPASEHLCDRNSKPSDIASCYIDCSGRKWNYGEWTSCSETCGSNGKMHRKSYCVDDSNRRVDESLCGREQKEATERECNRIPCPRWVYGHWSECSRSCDGGVKMRHAQCLDAADRETHTSRCGPAQTQEHCNEHACTWWQFGVWSDCSAKCGDGVQYRDANCTDRHRSVLPEHRCLKMEKIITKPCHRESCPKYKLGEWSQCSVSCEDGWSSRRVSCVSGNGTEVDMSLCGTASDRPASHQTCNLGTCPFWRNTDWSACSVSCGIGHRERTTECIYREQSVDASFCGDTKMPETSQTCHLLPCTSWKPSHWSPCSVTCGSGIQTRSVSCTRGSEGTIVDEYFCDRNTRPRLKKTCEKDTCDGPRVLQKLQADVPPIRWATGPWTACSATCGNGTQRRLLKCRDHVRDLPDEYCNHLDKEVSTRNCRLRDCSYWKMAEWEECPATCGTHVQQSRNVTCVSAEDGGRTILKDVDCDVQKRPTSARNCRLEPCPKGEEHIGSWIIGDWSKCSASCGGGWRRRSVSCTSSSCDETRKPKMFDKCNEELCPPLTNNSWQISPWTHCSVSCGGGVQRRKIWCEDVLSGRKQDDIECSEIKPREQRDCEMPPCRSHYHNKTSSASMTSLSSSNSNTTSSASASSLPILPPVVSWQTSAWSACSAKCGRGTKRRVVECVNPSLNVTVASTECDQTKKPVEEVRCRTKHCPRWKTTTWSSCSVTCGRGIRRREVQCYRGRKNLVSDSECNPKTKLNSVANCFPVACPAYRWNVTPWSKCKDECARGQKQTRRVHCISTSGKRAAPRMCELARAPTSIRECDTSNCPYEWVPGDWQTCSKSCGEGVQTREVRCRRKINFNSTIPIIFMLEDEPAVPKEKCELFPKPNESQTCELNPCDSEFKWSFGPWGECSKNCGQGIRRRRVKCVANDGRRVERVKCTTKKPRRTQYCFERNCLPSTCQELKSQNVKAKDGNYTILLDGFTIEIYCHRMNSTIPKAYLNVNPRTNFAEVYGKKLIYPHTCPFNGDRNDSCHCSEDGDASAGLTRFNKVRIDLLNRKFHLADYTFAKREYGVHVPYGTAGDCYSMKDCPQGIFSIDLKSAGLKLVDDLNWEDQGHRTSSRIDRFYNNAKVIGHCGGFCGKCSPERYKGLIFEVNTKLLNHVKNGGHIDDELDDDGFSGDMD |
| Enzyme Length | 2150 |
| Uniprot Accession Number | Q19791 |
| Absorption | |
| Active Site | ACT_SITE 425; /evidence=ECO:0000255|PROSITE-ProRule:PRU00276 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Secreted metalloprotease required for distal tip cell (DTC) migration along the body wall basement membranes, a key step that promotes gonad morphogenesis (PubMed:10588887, PubMed:10376599, PubMed:15556862). Probably acts by remodeling the basement membrane during cell migration (PubMed:10376599). Required to restrict presynaptic growth at the neuromuscular junctions (NMJ) in late larval stage and in adult motor neurons, probably by controlling collagen IV emb-9 degradation, a component of the synapse basement membrane (PubMed:25080592, PubMed:25232106). Also involved in the organization of adult muscle morphology (PubMed:25232106). Has a protease-independent function in promoting the transport from the endoplasmic reticulum to the Golgi apparatus of a variety of secretory cargos (PubMed:22419820, PubMed:26218657). Required for the secretion of insulin-like peptide ins-7, daf-28 and ins-18 and TGF beta-like protein daf-7 (PubMed:26218657). In peripheral tissues, negatively regulates insulin-mediated daf-16 translocation and thereby negatively regulates lifespan and dauer formation (PubMed:26218657). {ECO:0000269|PubMed:10376599, ECO:0000269|PubMed:10588887, ECO:0000269|PubMed:15556862, ECO:0000269|PubMed:22419820, ECO:0000269|PubMed:25080592, ECO:0000269|PubMed:25232106, ECO:0000269|PubMed:26218657}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (11); Domain (20); Glycosylation (18); Metal binding (3); Mutagenesis (1); Propeptide (1); Region (1); Signal peptide (1) |
| Keywords | Basement membrane;Developmental protein;Disulfide bond;ER-Golgi transport;Endoplasmic reticulum;Extracellular matrix;Glycoprotein;Golgi apparatus;Hydrolase;Metal-binding;Metalloprotease;Protease;Protein transport;Reference proteome;Repeat;Secreted;Signal;Transport;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane {ECO:0000269|PubMed:10376599}. Endoplasmic reticulum {ECO:0000269|PubMed:22419820}. Golgi apparatus {ECO:0000269|PubMed:22419820}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..28; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10778742; 11381264; 12097347; 12514189; 12529635; 14551910; 15338614; 17090602; 17164286; 17486083; 17850180; 19098902; 19343510; 20308279; 20439774; 21177967; 21367940; 22267497; 22286215; 22347378; 22560298; 22634595; 23800452; 24884423; 25487147; 33264296; 6593563; |
| Motif | |
| Gene Encoded By | |
| Mass | 242,582 |
| Kinetics | |
| Metal Binding | METAL 424; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU00276; METAL 428; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU00276; METAL 434; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU00276 |
| Rhea ID | |
| Cross Reference Brenda |