| IED ID | IndEnz0002016160 |
| Enzyme Type ID | protease016160 |
| Protein Name |
Hementerin EC 3.4.-.- Fragments |
| Gene Name | |
| Organism | Haementeria depressa (Leech) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Spiralia Lophotrochozoa Annelida Clitellata Hirudinea (leeches) Rhynchobdellida Glossiphoniidae Haementeria Haementeria depressa (Leech) |
| Enzyme Sequence | XTLSEPEPTCSIEYFRYQAIEDCEYSISVK |
| Enzyme Length | 30 |
| Uniprot Accession Number | P86682 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Fibrino(geno)lytic activity inhibited by EDTA but not by PMSF, E-64, 6-AHA and aprotinin. {ECO:0000269|PubMed:9684802}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.-.- |
| Enzyme Function | FUNCTION: Cleaves fibrinogen Aalpha (FGA), gamma (FGG) and Bbeta (FGB) chains. Degrades cross-linked fibrin. Has no amidolytic, plasminogenolytic or caseinolytic activity. Inhibits platelet aggregation induced by collagen (IC(50)=7.5ug/ml) and various other agonists, presumably via activation of a nitridergic pathway. Inhibition is accompanied by reduced ATP release from and surface expression of SELP and CD63 on platelets as well as increased intracellular levels of Ca(2+), cGMP and nitric oxide synthase activity. {ECO:0000269|PubMed:14515997, ECO:0000269|PubMed:9684802}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Non-adjacent residues (2); Non-terminal residue (1) |
| Keywords | Blood coagulation;Calcium;Direct protein sequencing;Fibrinolysis;Hemostasis;Hydrolase;Metalloprotease;Protease;Secreted |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 3,513 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |