| IED ID | IndEnz0002016215 |
| Enzyme Type ID | protease016215 |
| Protein Name |
Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe EC 2.4.1.222 O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase |
| Gene Name | lfng |
| Organism | Xenopus laevis (African clawed frog) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amphibia Batrachia Anura Pipoidea Pipidae Xenopodinae Xenopus Xenopus Xenopus laevis (African clawed frog) |
| Enzyme Sequence | MLKNWGKKLLLSIVGATLTCLLVLVVDQQSRHMLETQSDHEPGSAAAVHLRADLDPANPGDGGDPANSAQDSGTFSAYFNKLTRVRRDVEQVAAPSKDSAAPEEDITANDVFIAVKTTKKFHRSRMDLLMDTWISRNKEQTFIFTDGEDEELQKKTGNVISTNCSAAHSRQALSCKMAVEYDKFIESDKKWFCHVDDDNYVNVRTLVKLLSRYSHTNDIYIGKPSLDRPIQATERISESNMRPVNFWFATGGAGFCISRGLALKMSPWASGGHFMNTAEKIRLPDDCTIGYIIESVLGVKLIRSNLFHSHLENLHQVPQSEIHNQVTLSYGMFENKRNAILMKGAFSVEEDPSRFRSVHCLLYPDTPWCPWKAAY |
| Enzyme Length | 375 |
| Uniprot Accession Number | P79948 |
| Absorption | |
| Active Site | ACT_SITE 286; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | BINDING 125; /note=Substrate; /evidence=ECO:0000250; BINDING 197; /note=Substrate; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor.; EC=2.4.1.222; |
| DNA Binding | |
| EC Number | 2.4.1.222 |
| Enzyme Function | FUNCTION: Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Essential mediator of somite segmentation and patterning (By similarity). May be involved in mesoderm development (PubMed:8662522). {ECO:0000250|UniProtKB:O09010, ECO:0000269|PubMed:8662522}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Binding site (2); Chain (1); Disulfide bond (3); Glycosylation (1); Metal binding (2); Region (1); Site (1); Topological domain (2); Transmembrane (1) |
| Keywords | Developmental protein;Disulfide bond;Glycoprotein;Glycosyltransferase;Golgi apparatus;Manganese;Membrane;Metal-binding;Signal-anchor;Transferase;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | PTM: A soluble form may be derived from the membrane form by proteolytic processing. {ECO:0000305}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 42,134 |
| Kinetics | |
| Metal Binding | METAL 198; /note=Manganese; /evidence=ECO:0000250; METAL 310; /note=Manganese; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |