| IED ID | IndEnz0002016237 |
| Enzyme Type ID | protease016237 |
| Protein Name |
Macrodontain-2 EC 3.4.22.- Macrodontain II Fragment |
| Gene Name | |
| Organism | Ananas macrodontes (False pineapple) (Pseudananas macrodontes) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Bromeliaceae Bromelioideae Ananas Ananas macrodontes (False pineapple) (Pseudananas macrodontes) |
| Enzyme Sequence | AVPQSIDWRDYGAVNEVKNQNPCGSCW |
| Enzyme Length | 27 |
| Uniprot Accession Number | P83447 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by the general cysteine protease inhibitor E-64 (L-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane). {ECO:0000269|PubMed:11517945}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Cysteine protease that shows preferential cleavage as follows: Ala-|-Xaa > Gln-|-Xaa > Tyr-Xaa. Hydrolyzes the synthetic peptide substrate Bz-Phe-Val-Arg-pNA. {ECO:0000269|PubMed:11517945}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.5-8.5. {ECO:0000269|PubMed:11517945}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Non-terminal residue (1) |
| Keywords | Direct protein sequencing;Hydrolase;Protease;Thiol protease |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 3,037 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=8.9 uM for Bz-Phe-Val-Arg-pNA {ECO:0000269|PubMed:11517945}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |