| IED ID | IndEnz0002016325 |
| Enzyme Type ID | protease016325 |
| Protein Name |
Matrix metalloproteinase-18 MMP-18 EC 3.4.24.- Collagenase-4 xCol4 |
| Gene Name | mmp18 col4 |
| Organism | Xenopus laevis (African clawed frog) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amphibia Batrachia Anura Pipoidea Pipidae Xenopodinae Xenopus Xenopus Xenopus laevis (African clawed frog) |
| Enzyme Sequence | MNSLLLKLLLCVAITAAFPADKQDEPPATKEEMAENYLKRFYSLGTDGGPVGRKKHIQPFTEKLEQMQKFFGLKVTGTLDPKTVEVMEKPRCGVYDVGQYSTVAKSSAWQKKDLTYRILNFTPDLPQADVETAIQRAFKVWSDVTPLTFTRIYNEVSDIEISFTAGDHKDNSPFDGSGGILAHAFQPGNGIGGDAHFDEDETWTKTSEIYNLFLVAAHEFGHSLGLSHSTDQGALMYPTYSNTDPKTFQLPQDDINAIQYLYGKSSNPVQPTGPSTPSRCDPNVVFNAVTTMRGELIFFVKRFLWRKHPQASEAELMFVQAFWPSLPTNIDAAYENPITEQILVFKGSKYTALDGFDVVQGYPRNIYSLGFPKTVKRIDAAVHIEQLGKTYFFAAKKYWSYDEDKKQMDKGFPKQISNDFPGIPDKIDAAFYYRGRLYFFIGRSQFEYNINSKRIVQVLRSNSWLGC |
| Enzyme Length | 467 |
| Uniprot Accession Number | O13065 |
| Absorption | |
| Active Site | ACT_SITE 219; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | ACTIVITY REGULATION: Up-regulated in the tail by thyroid hormone. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Cleaves collagen type I. May play a role in larval tissue degeneration and adult organogenesis during amphibian metamorphosis. May be involved in tail resorption. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (1); Metal binding (4); Motif (1); Propeptide (1); Repeat (4); Signal peptide (1) |
| Keywords | Calcium;Collagen degradation;Disulfide bond;Extracellular matrix;Hydrolase;Metal-binding;Metalloprotease;Protease;Repeat;Secreted;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..17; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 90..97; /note=Cysteine switch; /evidence=ECO:0000250 |
| Gene Encoded By | |
| Mass | 52,813 |
| Kinetics | |
| Metal Binding | METAL 92; /note=Zinc; in inhibited form; /evidence=ECO:0000250; METAL 218; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 222; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 228; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Rhea ID | |
| Cross Reference Brenda |