| IED ID | IndEnz0002016327 |
| Enzyme Type ID | protease016327 |
| Protein Name |
Inositol phosphorylceramide synthase regulatory subunit KEI1 ICP synthase regulatory subunit KEI1 KEX2-cleavable protein essential for inositol phosphorylceramide synthesis Cleaved into: KEI1N; KEI1C |
| Gene Name | KEI1 YDR367W |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MRSSLLTLPKSFLGFMPLYLAVEIVLGISILNKCSGAYGILALFTGHPLDFMQWIAYLWSVFTLIVFSQGLYLIHKPNLLVFSQICVLYTIDTISTCFFTLWFTTQWFTLEDTANIDGNNALQSNPISTGKLTERGIDISKQSATESYEYTMTILITLVSLIFRFYFNFILASFVQELLHHPKYLVDRDDVEQNLKNKPIWKRLWAKSQKGCYKLCKNLLE |
| Enzyme Length | 221 |
| Uniprot Accession Number | Q06346 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Regulatory component of the inositol phosphorylceramide (ICP) synthase which catalyzes the addition of a phosphorylinositol group onto ceramide to form inositol phosphorylceramide, an essential step in sphingolipid biosynthesis. Helps the medial Golgi localization of IPC synthase in a COPI vesicle-dependent manner. {ECO:0000269|PubMed:19726565}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (3); Erroneous gene model prediction (1); Frameshift (1); Mutagenesis (2); Region (1); Transmembrane (4) |
| Keywords | Golgi apparatus;Lipid metabolism;Membrane;Reference proteome;Sphingolipid metabolism;Transmembrane;Transmembrane helix |
| Interact With | P36107 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:19726565}; Multi-pass membrane protein {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:19726565}. |
| Modified Residue | |
| Post Translational Modification | PTM: The precursor protein is cleaved into two polypeptide chains, KEI1N and KEI1C. The cleavage is performed in the Golgi apparatus by the KEX2 protease which recognizes residue Arg-135. Generation of KEX2 cleavage site may have been an accidental event in evolution without specific advantages or disadvantages in IPC synthesis. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11283351; 12140549; 12785717; 14690591; 19536198; 21603271; 23620734; 23737533; 24103214; 24220500; 24520995; 25750665; 26703187; 27664439; |
| Motif | |
| Gene Encoded By | |
| Mass | 25,484 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 2.7.1.227; |