IED Industry Enzyme Database

Detail Information for IndEnz0002016328
IED ID IndEnz0002016328
Enzyme Type ID protease016328
Protein Name Stromelysin-1
SL-1
EC 3.4.24.17
Matrix metalloproteinase-3
MMP-3
Transin-1
Gene Name MMP3 STMY1
Organism Homo sapiens (Human)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human)
Enzyme Sequence MKSLPILLLLCVAVCSAYPLDGAARGEDTSMNLVQKYLENYYDLKKDVKQFVRRKDSGPVVKKIREMQKFLGLEVTGKLDSDTLEVMRKPRCGVPDVGHFRTFPGIPKWRKTHLTYRIVNYTPDLPKDAVDSAVEKALKVWEEVTPLTFSRLYEGEADIMISFAVREHGDFYPFDGPGNVLAHAYAPGPGINGDAHFDDDEQWTKDTTGTNLFLVAAHEIGHSLGLFHSANTEALMYPLYHSLTDLTRFRLSQDDINGIQSLYGPPPDSPETPLVPTEPVPPEPGTPANCDPALSFDAVSTLRGEILIFKDRHFWRKSLRKLEPELHLISSFWPSLPSGVDAAYEVTSKDLVFIFKGNQFWAIRGNEVRAGYPRGIHTLGFPPTVRKIDAAISDKEKNKTYFFVEDKYWRFDEKRNSMEPGFPKQIAEDFPGIDSKIDAVFEEFGFFYFFTGSSQLEFDPNAKKVTHTLKSNSWLNC
Enzyme Length 477
Uniprot Accession Number P08254
Absorption
Active Site ACT_SITE 219
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Preferential cleavage where P1', P2' and P3' are hydrophobic residues.; EC=3.4.24.17;
DNA Binding
EC Number 3.4.24.17
Enzyme Function FUNCTION: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Beta strand (17); Chain (1); Compositional bias (1); Disulfide bond (1); Helix (8); Metal binding (24); Motif (1); Natural variant (1); Propeptide (1); Region (1); Repeat (4); Sequence conflict (1); Signal peptide (1); Turn (1)
Keywords 3D-structure;Calcium;Collagen degradation;Direct protein sequencing;Disulfide bond;Extracellular matrix;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Repeat;Secreted;Signal;Zinc;Zymogen
Interact With P50222
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..17; /evidence=ECO:0000305|PubMed:3360803
Structure 3D NMR spectroscopy (9); X-ray crystallography (33)
Cross Reference PDB 1B3D; 1B8Y; 1BIW; 1BM6; 1BQO; 1C3I; 1C8T; 1CAQ; 1CIZ; 1CQR; 1D5J; 1D7X; 1D8F; 1D8M; 1G05; 1G49; 1G4K; 1HFS; 1HY7; 1OO9; 1QIA; 1QIC; 1SLM; 1SLN; 1UEA; 1UMS; 1UMT; 1USN; 2D1O; 2JNP; 2JT5; 2JT6; 2SRT; 2USN; 3OHL; 3OHO; 3USN; 4DPE; 4G9L; 4JA1; 6MAV; 6N9D;
Mapped Pubmed ID 10229672; 10351963; 10551873; 10579818; 10639284; 10669564; 10809722; 10877850; 10966814; 10986281; 11104795; 11112695; 11116068; 11119712; 11150165; 11278729; 11297453; 11316871; 11327577; 11375993; 11380116; 11438501; 11546917; 11577169; 11825873; 11836255; 11841844; 11928810; 11960372; 11977998; 11982752; 11988625; 12009331; 12034345; 12034715; 12054564; 12071839; 12103254; 12124858; 12204805; 12205736; 12229968; 12230559; 12364729; 12385578; 12421825; 12432557; 12473595; 12485468; 12572877; 12634064; 12684035; 12727228; 12736398; 12750310; 12761889; 12784383; 12821236; 12832381; 12834347; 12837283; 12866026; 12867428; 12876636; 12952836; 1311314; 13129650; 1326552; 1371271; 14563588; 14712311; 14715248; 14764897; 1480033; 14984923; 14998290; 15009479; 15033492; 15070833; 15084374; 15094779; 15102660; 15116091; 15142265; 15161710; 15194213; 15203551; 15234427; 15274394; 15288468; 15290728; 15308783; 15312099; 15319295; 15319302; 15337261; 15342709; 15355616; 15383690; 15389640; 15467919; 15474069; 15528217; 15529384; 15546966; 15610507; 15665388; 15667946; 15701845; 15716694; 15748780; 15757531; 15763339; 15823277; 15863497; 15868410; 15879464; 15944607; 15950618; 15955221; 15993717; 16080875; 16082623; 16088212; 16100452; 16102106; 16158251; 16202315; 16205632; 16236134; 16238676; 16278009; 16302209; 16311244; 16323393; 16405530; 16424284; 16430740; 1649600; 16516860; 16532917; 16603129; 16606674; 16615041; 16629857; 16677108; 16739355; 16802342; 16822591; 16825321; 16871440; 16899023; 16904077; 16905683; 16919028; 16935611; 16937230; 16972019; 16972255; 16972994; 16984617; 1698775; 16998253; 17000679; 17000887; 17003494; 17024375; 17027562; 17033924; 1704217; 17058024; 17065356; 17077200; 17088321; 17125518; 17137622; 17145822; 17151781; 17178858; 17182940; 17198194; 17316652; 17316907; 17319946; 17320799; 17373931; 17381556; 17383306; 17434489; 17436000; 17471097; 17473191; 17502998; 17525128; 17530713; 17537400; 17543900; 17559909; 17564313; 17572495; 17589947; 17607721; 17617834; 17617837; 17664256; 17669621; 17672933; 17703334; 17710450; 17719307; 17763214; 17763953; 17765638; 1783204; 17875574; 17893005; 17901377; 17911432; 17919326; 17922906; 17929133; 17942123; 17958893; 17975717; 18006768; 18007247; 18028894; 18035073; 18063811; 18076359; 18077489; 18091353; 18172013; 18212480; 18220784; 18225577; 18234553; 18237197; 18262287; 18275497; 18303200; 18308831; 18342317; 18363112; 18426080; 18445105; 18469698; 18485900; 18496696; 18513389; 18541955; 18566588; 18571835; 18573993; 18606478; 18609140; 18619594; 18634015; 18636124; 18674944; 18674955; 1874716; 18760468; 18801353; 18810583; 18818437; 18818748; 18830194; 18974705; 19005290; 19008710; 19019335; 19019896; 19042922; 19056482; 19064570; 19065524; 19100060; 19124156; 19131662; 19132754; 19138696; 19155066; 19159011; 19165649; 19180518; 19185846; 19212832; 19221176; 19238444; 19245826; 19263529; 19268777; 19275880; 19279308; 19293200; 19318431; 19321798; 19336475; 19337798; 19376102; 19381070; 19432148; 19435861; 19438845; 19479237; 19504098; 19507017; 19507256; 19508478; 19522160; 19527514; 19551141; 19559392; 19576586; 19578796; 19585142; 19614693; 19628284; 19643940; 19664242; 19690958; 19729601; 19731200; 19762026; 19770597; 19778329; 19786072; 19789190; 19812315; 19834535; 19836620; 19843588; 19892918; 19906289; 19913121; 19923455; 19948975; 19949639; 20009418; 20038976; 2005102; 20082876; 20092902; 20113256; 20142115; 20142769; 20211321; 20215736; 20302034; 20346360; 20359947; 20360864; 20374310; 20385826; 20435584; 20436380; 20437586; 20452482; 20453000; 20455995; 20467172; 20472558; 20483790; 20484597; 20485444; 20493732; 20538124; 20541269; 20574775; 20584750; 20587546; 20609072; 20609440; 20616161; 20616708; 20622166; 20628086; 20628624; 20630073; 20640910; 20654099; 20655856; 20672350; 20673868; 20707923; 20708412; 20731042; 20740392; 20802378; 20822909; 20827277; 20853681; 20873121; 20935575; 20936527; 20942234; 20948207; 20959880; 21037509; 21044079; 21048031; 21055468; 21103065; 21108787; 21111627; 21113741; 21134112; 21150162; 21183746; 21187770; 21190865; 21206012; 21212676; 21219546; 21228692; 21232745; 21244504; 21247370; 21277817; 21321996; 21330369; 21336947; 21345293; 21357403; 21410539; 21431945; 21443102; 21451793; 21454617; 21454822; 21481333; 21601886; 21667159; 2169257; 2169335; 21707906; 2173606; 21753786; 21770773; 21782982; 21784848; 21802713; 21867693; 21871427; 21872914; 21900582; 21917825; 21925226; 21935932; 21948134; 22027013; 22057989; 22089392; 22092736; 22114952; 22121090; 22122951; 22134873; 22175304; 22184093; 22204652; 22207289; 22210296; 22222271; 22241750; 22265821; 22314025; 22323068; 22441965; 22455335; 22457004; 22515887; 22531699; 2253219; 22587600; 22628530; 22671570; 22730240; 22776467; 22786680; 22819245; 22836729; 22851691; 22862420; 22868188; 22888638; 22890115; 22922035; 22927007; 22965799; 22975753; 23086340; 23100088; 23108733; 23123160; 23128460; 23328930; 23343931; 23357697; 23377317; 23409137; 23412981; 23483952; 23509928; 23522322; 23548063; 23555662; 23564449; 23575435; 23608488; 23637955; 23660647; 23696797; 23705892; 23707809; 23744166; 23752352; 23769926; 23794948; 23872201; 23922934; 23926017; 23934131; 23936524; 23941979; 23967200; 23975061; 23978194; 24043512; 24079541; 24101259; 24111521; 24146820; 24168165; 24338609; 24349530; 24374826; 24414606; 24416274; 24464212; 24489939; 24577893; 24583398; 24670135; 24743777; 24799419; 24804215; 24850902; 24939159; 24999605; 25135219; 25135220; 25147433; 25168209; 25177880; 25217476; 25234944; 25263962; 25291965; 25292126; 25292349; 25340798; 25351421; 2537292; 25391977; 25403368; 25416956; 25463899; 2548603; 2551898; 25564266; 25599395; 25605062; 25611261; 25711032; 25742789; 25807652; 25818544; 25854159; 25912212; 25933064; 25993872; 26087627; 26109466; 26123623; 26125893; 26156043; 26191285; 26206176; 26260627; 26314579; 26437765; 26510114; 26526578; 26634533; 26681054; 26712881; 26713439; 26754027; 26805660; 26850593; 26853425; 26863055; 27013197; 27043485; 27051023; 27061553; 27072559; 27089651; 27108649; 27129069; 27145630; 27161754; 27173221; 27176648; 27177702; 27211292; 27268637; 27389714; 27391467; 27423606; 27438616; 27611862; 27659327; 27659332; 27706715; 27733582; 27771306; 27793533; 27797337; 27837634; 27911281; 27975070; 28002595; 28158775; 28160256; 28337851; 2834383; 28365812; 28396463; 28432524; 28445942; 28447732; 2845110; 28461705; 28497435; 28521653; 28676856; 28718374; 28781337; 28798935; 28819304; 28831065; 28850021; 28914997; 28944430; 28961241; 29044936; 29070037; 29120572; 29141665; 29164307; 29165854; 29172775; 29276151; 29304854; 29317790; 29358561; 29390034; 29421776; 29442475; 29458338; 29462373; 29483840; 29670668; 29738289; 29883760; 30036111; 30117779; 30138480; 30187662; 30288688; 30297859; 3038863; 30406565; 30422384; 30509760; 3058116; 30732526; 30755371; 30856693; 3095317; 31040180; 31055827; 31081124; 31124320; 31208828; 31218540; 31356534; 31377584; 31396295; 31467432; 31525531; 31652448; 31827179; 31833734; 31882488; 31887330; 31894328; 31955640; 32093874; 32157746; 3223920; 32272349; 32325785; 32417757; 32421262; 32450244; 32603484; 32610117; 32945390; 33130223; 33139684; 33157227; 33170424; 33243974; 33319933; 33337838; 33404658; 33531109; 33986628; 34198485; 34222497; 34233423; 34257518; 34367126; 34445715; 34980041; 6258630; 6270089; 6322761; 7547873; 7669817; 7780967; 7826345; 7896811; 7981201; 8216228; 8223643; 8226919; 8305481; 8463259; 8576151; 8580839; 8603731; 8621622; 8626565; 8668165; 8920930; 9008168; 9065415; 9092507; 9115292; 9148753; 9346290; 9395517; 9428515; 9578462; 9651395; 9733482; 9873489;
Motif MOTIF 90..97; /note=Cysteine switch; /evidence=ECO:0000250
Gene Encoded By
Mass 53,977
Kinetics
Metal Binding METAL 92; /note=Zinc 2; in inhibited form; METAL 124; /note=Calcium 1; METAL 158; /note=Calcium 2; METAL 168; /note=Zinc 1; METAL 170; /note=Zinc 1; METAL 175; /note=Calcium 3; METAL 176; /note=Calcium 3; via carbonyl oxygen; METAL 178; /note=Calcium 3; via carbonyl oxygen; METAL 180; /note=Calcium 3; via carbonyl oxygen; METAL 183; /note=Zinc 1; METAL 190; /note=Calcium 2; via carbonyl oxygen; METAL 192; /note=Calcium 2; via carbonyl oxygen; METAL 194; /note=Calcium 2; METAL 196; /note=Zinc 1; METAL 198; /note=Calcium 3; METAL 199; /note=Calcium 1; METAL 201; /note=Calcium 1; METAL 201; /note=Calcium 3; METAL 218; /note=Zinc 2; catalytic; /evidence=ECO:0000269|PubMed:8740360; METAL 222; /note=Zinc 2; catalytic; /evidence=ECO:0000269|PubMed:8740360; METAL 228; /note=Zinc 2; catalytic; /evidence=ECO:0000269|PubMed:8740360; METAL 297; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250; METAL 389; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250; METAL 438; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250
Rhea ID
Cross Reference Brenda 3.4.24.17;