IED Industry Enzyme Database

Detail Information for IndEnz0002016331
IED ID IndEnz0002016331
Enzyme Type ID protease016331
Protein Name Matrilysin
EC 3.4.24.23
Matrin
Matrix metalloproteinase-7
MMP-7
Pump-1 protease
Uterine metalloproteinase
Gene Name MMP7 MPSL1 PUMP1
Organism Homo sapiens (Human)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human)
Enzyme Sequence MRLTVLCAVCLLPGSLALPLPQEAGGMSELQWEQAQDYLKRFYLYDSETKNANSLEAKLKEMQKFFGLPITGMLNSRVIEIMQKPRCGVPDVAEYSLFPNSPKWTSKVVTYRIVSYTRDLPHITVDRLVSKALNMWGKEIPLHFRKVVWGTADIMIGFARGAHGDSYPFDGPGNTLAHAFAPGTGLGGDAHFDEDERWTDGSSLGINFLYAATHELGHSLGMGHSSDPNAVMYPTYGNGDPQNFKLSQDDIKGIQKLYGKRSNSRKK
Enzyme Length 267
Uniprot Accession Number P09237
Absorption
Active Site ACT_SITE 215
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Cleavage of 14-Ala-|-Leu-15 and 16-Tyr-|-Leu-17 in B chain of insulin. No action on collagen types I, II, IV, V. Cleaves gelatin chain alpha-2(I) > alpha-1(I).; EC=3.4.24.23;
DNA Binding
EC Number 3.4.24.23
Enzyme Function FUNCTION: Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. {ECO:0000269|PubMed:2550050}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Beta strand (16); Chain (1); Helix (8); Metal binding (18); Motif (1); Natural variant (3); Propeptide (1); Signal peptide (1); Turn (2)
Keywords 3D-structure;Calcium;Collagen degradation;Direct protein sequencing;Extracellular matrix;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Zinc;Zymogen
Interact With P17931
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..17; /evidence=ECO:0000269|PubMed:2253219
Structure 3D NMR spectroscopy (6); X-ray crystallography (5)
Cross Reference PDB 1MMP; 1MMQ; 1MMR; 2DDY; 2MZE; 2MZH; 2MZI; 2Y6C; 2Y6D; 5UE2; 5UE5;
Mapped Pubmed ID 10551873; 10809722; 10966814; 10986281; 11112695; 11119712; 11375993; 11577169; 11701474; 11801551; 11825873; 11841844; 11872628; 11925859; 11927011; 11928810; 11983918; 12005165; 12034345; 12112311; 12421825; 12464266; 12579270; 12684035; 12684625; 12759241; 12759346; 12808021; 12958188; 12963695; 1311314; 1326552; 1371271; 1390635; 14516315; 14744783; 14764897; 1480033; 15040016; 15102692; 15149334; 15239678; 15375490; 15523695; 15618645; 15696117; 15725655; 15800927; 15809719; 15860507; 15866216; 15868410; 15930031; 15950618; 15979995; 16097959; 16115946; 16142384; 16248458; 16278009; 16286510; 16356191; 16405530; 16455621; 16474169; 16474379; 16476739; 16494848; 1649600; 16631740; 16804904; 16825321; 16940985; 16956593; 1698775; 17009118; 17029196; 17038551; 1707285; 17088321; 17114341; 17125518; 17129995; 17145820; 17145868; 17153464; 17209789; 17219436; 17310281; 17373931; 17396032; 17487834; 17502620; 17507989; 17525128; 17554258; 17564313; 17607721; 17671679; 17672933; 17695544; 17718429; 17728005; 18006768; 18023162; 18036564; 18202161; 18214300; 18308831; 18329694; 18340552; 18364512; 18385523; 18411043; 18422740; 18447576; 18448157; 18485588; 18500535; 18511876; 18548257; 18563468; 18600430; 18636198; 18644839; 18648013; 18653469; 18674869; 18695873; 18713744; 18715618; 18721140; 1874716; 18768525; 18798254; 18805052; 18823373; 18931651; 18955490; 18958543; 18974372; 19000448; 19016757; 19020757; 19022775; 19094228; 19121849; 19132754; 19138696; 19173304; 19181662; 19221016; 19231585; 19266094; 19303106; 19317620; 19329997; 19360357; 19420105; 19421758; 19435861; 19505922; 19586554; 19596921; 19608871; 19634113; 19643940; 19654318; 19676133; 19679556; 19682489; 19690403; 19690958; 19701966; 19724852; 19728912; 19730683; 19785773; 19787626; 19789190; 19812315; 19834535; 19929564; 20007614; 20040080; 20113256; 20132413; 20139113; 20142769; 20146992; 20156184; 20180812; 20192597; 20219015; 20230842; 20300917; 20345904; 20347338; 20359117; 20360147; 20372795; 20375326; 20452482; 20473942; 20484597; 20491537; 20584750; 20587546; 20605361; 20605794; 20624652; 20662554; 20673868; 20680712; 20707923; 20730428; 20736794; 20813660; 20826916; 20827277; 20853162; 20872971; 20939893; 20979817; 21048031; 21055468; 21116502; 21165404; 21255765; 21302026; 21315773; 21336735; 21455340; 21481787; 21516345; 21520417; 21593056; 2169257; 2169335; 21707759; 21722074; 21764478; 21787888; 21814482; 21858178; 21901248; 21912055; 21935919; 21981819; 21999204; 22024063; 22042554; 22047080; 22051851; 22076613; 22089237; 22095947; 22113040; 22131018; 22200690; 22215634; 22227189; 22268124; 22292085; 22368250; 22417142; 22524815; 22582975; 22621753; 22631636; 22645147; 22645431; 22883281; 22936257; 22944341; 22957090; 22957785; 22974215; 23065739; 23076342; 23249718; 23300791; 23343931; 23344661; 23437250; 23562717; 23644699; 23682078; 23725125; 23783374; 23790336; 23803888; 23807291; 23845380; 23893803; 23895900; 23953525; 24043512; 24048760; 24143235; 24261884; 24270662; 24283384; 24400123; 24425843; 24469951; 24613844; 24615104; 24624896; 24632608; 24704993; 24755685; 24833109; 24847890; 24930385; 24980707; 24981249; 25063248; 25123263; 25241761; 25263962; 25300296; 25321193; 25338725; 25357219; 25368272; 2548603; 2551898; 25549795; 25579141; 25588786; 25607396; 25676403; 25823818; 25847246; 25875355; 25901368; 25919283; 26116564; 26164539; 26214021; 26229436; 26301605; 26402225; 26408312; 26412030; 26418251; 26439767; 26457789; 26464654; 26507660; 26520416; 26526578; 26553362; 26648518; 26652716; 26792722; 26862737; 26906940; 26950446; 27069188; 27078876; 27098106; 27146730; 27270030; 27293304; 27356744; 27409770; 27429508; 27434116; 27538541; 27556509; 27561093; 27611862; 27755977; 27827523; 27922675; 27987113; 28137415; 28241871; 28252644; 28333147; 2834383; 2845110; 28510611; 28648610; 28652430; 28699830; 28821612; 28870920; 28871443; 28885729; 28922659; 28944430; 29046355; 29187444; 29317790; 29476769; 29556339; 29558202; 29599326; 29631554; 29695403; 29721609; 29740048; 29770948; 29845775; 29880550; 30036379; 30187906; 30275186; 30303742; 30388078; 30414845; 30627896; 30689319; 30695426; 30809850; 30911281; 3095317; 30967475; 31129920; 31299414; 31299935; 31342296; 31350432; 31366549; 31576476; 31644669; 31771979; 31827648; 32014910; 32101136; 3223920; 32373960; 32377701; 32663776; 32892475; 32961536; 33005257; 33139675; 33237244; 33536476; 33581214; 33655961; 33713636; 33736747; 33809984; 34225019; 34588190; 34769248; 6258630; 7547873; 7669817; 7780967; 7896811; 7981201; 8015608; 8194591; 8216228; 8223643; 8226919; 8463259; 8603731; 8920930; 9008168; 9065415; 9092507; 9115292; 9148753; 9344615; 9346290; 9395517; 9428515; 9578462; 9651395;
Motif MOTIF 85..92; /note=Cysteine switch; /evidence=ECO:0000250
Gene Encoded By
Mass 29,677
Kinetics
Metal Binding METAL 87; /note=Zinc 2; in inhibited form; /evidence=ECO:0000250; METAL 153; /note=Calcium 1; METAL 163; /note=Zinc 1; METAL 165; /note=Zinc 1; METAL 170; /note=Calcium 2; METAL 171; /note=Calcium 2; via carbonyl oxygen; METAL 173; /note=Calcium 2; via carbonyl oxygen; METAL 175; /note=Calcium 2; via carbonyl oxygen; METAL 178; /note=Zinc 1; METAL 185; /note=Calcium 1; via carbonyl oxygen; METAL 187; /note=Calcium 1; via carbonyl oxygen; METAL 189; /note=Calcium 1; METAL 191; /note=Zinc 1; METAL 193; /note=Calcium 2; METAL 196; /note=Calcium 2; METAL 214; /note=Zinc 2; catalytic; METAL 218; /note=Zinc 2; catalytic; METAL 224; /note=Zinc 2; catalytic
Rhea ID
Cross Reference Brenda 3.4.24.23;