| IED ID | IndEnz0002016367 |
| Enzyme Type ID | protease016367 |
| Protein Name |
Bacteriophytochrome BphP XccBphP |
| Gene Name | bphP XC_4241 |
| Organism | Xanthomonas campestris pv. campestris (strain 8004) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Xanthomonadales Xanthomonadaceae Xanthomonas Xanthomonas campestris Xanthomonas campestris pv. campestris Xanthomonas campestris pv. campestris (strain 8004) |
| Enzyme Sequence | MSTATNPLDLDVCAREPIHIPGLIQPYGVLLVIDPADGRIVQASTTAADLLGVPMAALLGMPYTQVLTLPEAQPFAVDDQPQHLMHAEVRFPQRATPPASAWVAAWHLYPQQWLVEMEPRDARLLDVTLREAMPLLRSVERDPGIAEAAVRVAKGLRSLIGFDRVMIYRFDEEWNGDIIAEARKPELEAYLGLHYPASDIPAQARALYLRNRVRQIADVGYQPSPIQPTVHPQLGTPVDLSDVSLRSVSPVHLEYLANMGVTATLVASIVVNDALWGLISCHHYSPHFTNHAMRDVTDAVARTLAGRIGALQAVARARLESVLLTVREKLITDFNDAEHMTVELLDDMAPDLMDVVDADGVAIFHGNDISRHGTTPDVAALRRIRDHIESEHHEALREDAVGALHVDAIGEVFPELADLAPLAAGFIFVPLMPQSRSALLWTRREQIQQIKWAGNPQLAKLEDIPNSRLSPRKSFDLWQQTVRGRARRWSPLHLESARSLRVLIELMERKRFQQDFTLLEASLSRLRDGVAIIERGTANAAHRLLFVNTAFADVCGSDVAELIGRELQTLYASDAPRANVELLQDALRNGRAAYVTLPLQVSDGAPVYRQFHLEPLPSPSGVTAHWLLQLRDPE |
| Enzyme Length | 634 |
| Uniprot Accession Number | A0A0H2XCS3 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 13; /note="Biliverdin IX-alpha chromophore; covalent, via 1 link"; /evidence="ECO:0000269|PubMed:27107635" |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Photoreceptor which exists in two forms that are reversibly interconvertible by light: far-red light (733 nm) converts protein to the red-absorbing (Pr) form, while red light (630 nm) partly converts the protein to the far-red-absorbing (Pfr) form (PubMed:27107635). Regulates virulence of X.campestris pv. campestris on its host plants, perhaps by fine-tuning expression to ambient light levels and/or spatial cues (PubMed:27621284). The Pr form may sense light and partially inhibit virulence; in the dark (Pfr form) biofilm and xanathan production rise and bacteria are more virulent (PubMed:27621284). Strains overexpressing this protein have significantly decreased amounts of extracellular beta-1,4-endoglucanase, produce less xanthin and have decreased transcription of genes involved in virulence such as endoglucanases, type 2 secretion systems, xanthan production and flagellar-dependent motility (PubMed:27621284). {ECO:0000269|PubMed:27107635, ECO:0000269|PubMed:27621284, ECO:0000305|PubMed:27621284}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (33); Binding site (1); Chain (1); Domain (3); Helix (21); Mutagenesis (1); Region (4); Turn (3) |
| Keywords | 3D-structure;Chromophore;Photoreceptor protein;Receptor;Sensory transduction;Virulence |
| Interact With | |
| Induction | INDUCTION: Expressed in dark and light (at protein level). Part of the bphO-bphP operon (PubMed:27621284). {ECO:0000269|PubMed:27621284}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Contains one covalently linked biliverdin IX-alpha chromophore; present in the crystal structure as a mixture of Pr and Meta-R configurations. {ECO:0000269|PubMed:27107635}. |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (5) |
| Cross Reference PDB | 5AKP; 5UYR; 6NDO; 6NDP; 6PL0; |
| Mapped Pubmed ID | 27621284; 32683707; 33864705; 34818032; |
| Motif | |
| Gene Encoded By | |
| Mass | 70,355 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |