| IED ID | IndEnz0002016378 |
| Enzyme Type ID | protease016378 |
| Protein Name |
Major capsid protein Gene product 5 gp5 Major head protein Cleaved into: Scaffolding domain delta |
| Gene Name | 5 |
| Organism | Enterobacteria phage HK97 (Bacteriophage HK97) |
| Taxonomic Lineage | Viruses Duplodnaviria Heunggongvirae Uroviricota Caudoviricetes Caudovirales Siphoviridae (phages with long non-contractile tails) Hendrixvirinae Byrnievirus Enterobacteria phage HK97 (Bacteriophage HK97) |
| Enzyme Sequence | MSELALIQKAIEESQQKMTQLFDAQKAEIESTGQVSKQLQSDLMKVQEELTKSGTRLFDLEQKLASGAENPGEKKSFSERAAEELIKSWDGKQGTFGAKTFNKSLGSDADSAGSLIQPMQIPGIIMPGLRRLTIRDLLAQGRTSSNALEYVREEVFTNNADVVAEKALKPESDITFSKQTANVKTIAHWVQASRQVMDDAPMLQSYINNRLMYGLALKEEGQLLNGDGTGDNLEGLNKVATAYDTSLNATGDTRADIIAHAIYQVTESEFSASGIVLNPRDWHNIALLKDNEGRYIFGGPQAFTSNIMWGLPVVPTKAQAAGTFTVGGFDMASQVWDRMDATVEVSREDRDNFVKNMLTILCEERLALAHYRPTAIIKGTFSSGS |
| Enzyme Length | 385 |
| Uniprot Accession Number | P49861 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Assembles to form an icosahedral capsid of 66 nm, with a T=7 laevo symmetry (PubMed:11000116, PubMed:21276801). Responsible for its self-assembly into a procapsid. The phage does not need to encode a separate scaffolfing protein because its capsid protein contains the delta domain that carries that function. {ECO:0000269|PubMed:11000116, ECO:0000269|PubMed:21276801, ECO:0000269|PubMed:7669350, ECO:0000269|PubMed:7723020}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (15); Chain (2); Coiled coil (1); Cross-link (2); Helix (11); Mutagenesis (4); Turn (3) |
| Keywords | 3D-structure;Capsid protein;Coiled coil;Direct protein sequencing;Isopeptide bond;Late protein;Reference proteome;T=7 icosahedral capsid protein;Viral capsid assembly;Viral release from host cell;Virion |
| Interact With | P49860; Itself |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Virion. Note=Forms the icosahedral capsid shell which contains 420 major capsid proteins. {ECO:0000269|PubMed:11000116}. |
| Modified Residue | |
| Post Translational Modification | PTM: The scaffolding domain delta is cleaved by the viral protease and lost after assembly. {ECO:0000269|PubMed:7723020}.; PTM: The major capsid proteins are covalently cross-linked. {ECO:0000269|PubMed:11000116}. |
| Signal Peptide | |
| Structure 3D | Electron microscopy (4); X-ray crystallography (8) |
| Cross Reference PDB | 1IF0; 1OHG; 2FRP; 2FS3; 2FSY; 2FT1; 2FTE; 2GP1; 3DDX; 3E8K; 3J1A; 3QPR; |
| Mapped Pubmed ID | 10089306; 10930835; 15175152; |
| Motif | |
| Gene Encoded By | |
| Mass | 42,243 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |