| IED ID | IndEnz0002016399 |
| Enzyme Type ID | protease016399 |
| Protein Name |
Serralysin EC 3.4.24.40 Alkaline metalloproteinase AP |
| Gene Name | aprA PA1249 |
| Organism | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa group Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
| Enzyme Sequence | MSSNSLALKGRSDAYTQVDNFLHAYARGGDELVNGHPSYTVDQAAEQILREQASWQKAPGDSVLTLSYSFLTKPNDFFNTPWKYVSDIYSLGKFSAFSAQQQAQAKLSLQSWSDVTNIHFVDAGQGDQGDLTFGNFSSSVGGAAFAFLPDVPDALKGQSWYLINSSYSANVNPANGNYGRQTLTHEIGHTLGLSHPGDYNAGEGDPTYADATYAEDTRAYSVMSYWEEQNTGQDFKGAYSSAPLLDDIAAIQKLYGANLTTRTGDTVYGFNSNTERDFYSATSSSSKLVFSVWDAGGNDTLDFSGFSQNQKINLNEKALSDVGGLKGNVSIAAGVTVENAIGGSGSDLLIGNDVANVLKGGAGNDILYGGLGADQLWGGAGADTFVYGDIAESSAAAPDTLRDFVSGQDKIDLSGLDAFVNGGLVLQYVDAFAGKAGQAILSYDAASKAGSLAIDFSGDAHADFAINLIGQATQADIVV |
| Enzyme Length | 479 |
| Uniprot Accession Number | Q03023 |
| Absorption | |
| Active Site | ACT_SITE 186 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage of bonds with hydrophobic residues in P1'.; EC=3.4.24.40; Evidence={ECO:0000269|PubMed:4199986}; |
| DNA Binding | |
| EC Number | 3.4.24.40 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (27); Chain (1); Helix (13); Metal binding (45); Natural variant (11); Propeptide (1); Repeat (3); Turn (8) |
| Keywords | 3D-structure;Calcium;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Repeat;Secreted;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 1AKL; 1JIW; 1KAP; 3VI1; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 50,433 |
| Kinetics | |
| Metal Binding | METAL 185; /note=Zinc; via tele nitrogen; catalytic; METAL 189; /note=Zinc; via tele nitrogen; catalytic; METAL 195; /note=Zinc; via tele nitrogen; catalytic; METAL 262; /note=Calcium 1; via carbonyl oxygen; METAL 264; /note=Calcium 1; via carbonyl oxygen; METAL 266; /note=Calcium 1; METAL 294; /note=Calcium 1; METAL 296; /note=Calcium 1; via carbonyl oxygen; METAL 297; /note=Calcium 2; via carbonyl oxygen; METAL 299; /note=Calcium 1; METAL 299; /note=Calcium 2; METAL 336; /note=Calcium 2; via carbonyl oxygen; METAL 338; /note=Calcium 2; METAL 343; /note=Calcium 3; via carbonyl oxygen; METAL 345; /note=Calcium 3; via carbonyl oxygen; METAL 347; /note=Calcium 3; METAL 352; /note=Calcium 4; via carbonyl oxygen; METAL 354; /note=Calcium 4; via carbonyl oxygen; METAL 356; /note=Calcium 4; METAL 360; /note=Calcium 3; via carbonyl oxygen; METAL 361; /note=Calcium 5; via carbonyl oxygen; METAL 362; /note=Calcium 3; via carbonyl oxygen; METAL 363; /note=Calcium 5; via carbonyl oxygen; METAL 365; /note=Calcium 3; METAL 365; /note=Calcium 5; METAL 369; /note=Calcium 4; via carbonyl oxygen; METAL 370; /note=Calcium 6; via carbonyl oxygen; METAL 371; /note=Calcium 4; via carbonyl oxygen; METAL 372; /note=Calcium 6; via carbonyl oxygen; METAL 374; /note=Calcium 4; METAL 374; /note=Calcium 6; METAL 378; /note=Calcium 5; via carbonyl oxygen; METAL 379; /note=Calcium 7; via carbonyl oxygen; METAL 380; /note=Calcium 5; via carbonyl oxygen; METAL 381; /note=Calcium 7; via carbonyl oxygen; METAL 383; /note=Calcium 5; METAL 383; /note=Calcium 7; METAL 392; /note=Calcium 6; via carbonyl oxygen; METAL 399; /note=Calcium 6; METAL 409; /note=Calcium 7; METAL 455; /note=Calcium 8; METAL 457; /note=Calcium 8; METAL 459; /note=Calcium 8; METAL 461; /note=Calcium 8; via carbonyl oxygen; METAL 463; /note=Calcium 8 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.40; |