| IED ID | IndEnz0002016415 |
| Enzyme Type ID | protease016415 |
| Protein Name |
Desert hedgehog protein DHH HHG-3 Cleaved into: Desert hedgehog protein N-product; Desert hedgehog protein C-product |
| Gene Name | DHH |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MALLTNLLPLCCLALLALPAQSCGPGRGPVGRRRYARKQLVPLLYKQFVPGVPERTLGASGPAEGRVARGSERFRDLVPNYNPDIIFKDEENSGADRLMTERCKERVNALAIAVMNMWPGVRLRVTEGWDEDGHHAQDSLHYEGRALDITTSDRDRNKYGLLARLAVEAGFDWVYYESRNHVHVSVKADNSLAVRAGGCFPGNATVRLWSGERKGLRELHRGDWVLAADASGRVVPTPVLLFLDRDLQRRASFVAVETEWPPRKLLLTPWHLVFAARGPAPAPGDFAPVFARRLRAGDSVLAPGGDALRPARVARVAREEAVGVFAPLTAHGTLLVNDVLASCYAVLESHQWAHRAFAPLRLLHALGALLPGGAVQPTGMHWYSRLLYRLAEELLG |
| Enzyme Length | 396 |
| Uniprot Accession Number | O43323 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (7); Chain (3); Helix (7); Lipidation (2); Metal binding (12); Mutagenesis (1); Natural variant (1); Sequence conflict (1); Signal peptide (1); Site (4); Turn (1) |
| Keywords | 3D-structure;Autocatalytic cleavage;Calcium;Cell membrane;Developmental protein;Disease variant;Hydrolase;Lipoprotein;Membrane;Metal-binding;Palmitate;Protease;Reference proteome;Secreted;Signal;Zinc |
| Interact With | Q9BWV1; Q4KMG0; Q96QV1-1 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Desert hedgehog protein N-product]: Cell membrane {ECO:0000250}; Lipid-anchor {ECO:0000250}; Extracellular side {ECO:0000250}. Note=The N-terminal peptide remains associated with the cell surface. {ECO:0000250}.; SUBCELLULAR LOCATION: [Desert hedgehog protein C-product]: Secreted, extracellular space {ECO:0000250}. Note=The C-terminal peptide diffuses from the cell. {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | PTM: The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). This covalent modification appears to play an essential role in restricting the spatial distribution of the protein activity to the cell surface. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (By similarity). {ECO:0000250}. |
| Signal Peptide | SIGNAL 1..22; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (5) |
| Cross Reference PDB | 2WFQ; 2WFR; 2WG3; 3N1G; 3N1Q; |
| Mapped Pubmed ID | 10050855; 10619433; 10966113; 10984056; 11027307; 11486055; 11841368; 11990454; 12015973; 12123571; 12372301; 12569124; 15019948; 15075292; 15110707; 15163793; 15292211; 15294024; 15300262; 15618519; 15647319; 15963974; 16136078; 16282375; 16335952; 16390857; 16611729; 16630821; 16647304; 16980583; 17086203; 17126548; 17210683; 17504940; 17504941; 17507410; 17609110; 17641202; 17960137; 17967162; 18264092; 18477453; 18497258; 18502753; 18534984; 18559595; 18711132; 18788453; 18794898; 19002207; 19074837; 19081070; 19176481; 19196978; 19218434; 19346256; 19365551; 19561609; 19603532; 19948480; 20412775; 20585641; 20844013; 20844016; 21193222; 21257310; 21339334; 21357747; 21659505; 21664575; 21664576; 21664577; 21695114; 21802063; 21816240; 21842183; 22123293; 22277298; 22326621; 22467855; 22514309; 22609552; 22677548; 22902404; 22981989; 22986504; 23026747; 23112049; 23118222; 23719536; 23725801; 23786321; 23867461; 23867465; 23900540; 24127550; 24302888; 24582806; 24925320; 25088423; 25517604; 25927242; 28472341; 28708305; 30298535; 30355159; 31018998; 31240586; 32504121; 33712994; 33864885; 7885476; 7985023; 8689684; 8824192; 8898207; 8906787; 8906794; 9593755; |
| Motif | |
| Gene Encoded By | |
| Mass | 43,577 |
| Kinetics | |
| Metal Binding | METAL 90; /note="Calcium 1"; /evidence="ECO:0000269|PubMed:19561611, ECO:0000269|PubMed:20519495, ECO:0007744|PDB:2WFR, ECO:0007744|PDB:3N1G, ECO:0007744|PDB:3N1Q"; METAL 91; /note="Calcium 1"; /evidence="ECO:0000269|PubMed:19561611, ECO:0000269|PubMed:20519495, ECO:0007744|PDB:2WFR, ECO:0007744|PDB:3N1G, ECO:0007744|PDB:3N1Q"; METAL 91; /note="Calcium 2"; /evidence="ECO:0000269|PubMed:19561611, ECO:0000269|PubMed:20519495, ECO:0007744|PDB:2WFR, ECO:0007744|PDB:3N1G, ECO:0007744|PDB:3N1Q"; METAL 96; /note="Calcium 1"; /evidence="ECO:0000269|PubMed:19561611, ECO:0000269|PubMed:20519495, ECO:0007744|PDB:2WFR, ECO:0007744|PDB:3N1G, ECO:0007744|PDB:3N1Q"; METAL 126; /note="Calcium 1; via carbonyl oxygen"; /evidence="ECO:0000269|PubMed:19561611, ECO:0000269|PubMed:20519495, ECO:0007744|PDB:2WFR, ECO:0007744|PDB:3N1G, ECO:0007744|PDB:3N1Q"; METAL 127; /note="Calcium 1"; /evidence="ECO:0000269|PubMed:19561611, ECO:0000269|PubMed:20519495, ECO:0007744|PDB:2WFR, ECO:0007744|PDB:3N1G, ECO:0007744|PDB:3N1Q"; METAL 127; /note="Calcium 2"; /evidence="ECO:0000269|PubMed:19561611, ECO:0000269|PubMed:20519495, ECO:0007744|PDB:2WFR, ECO:0007744|PDB:3N1G, ECO:0007744|PDB:3N1Q"; METAL 130; /note="Calcium 2"; /evidence="ECO:0000269|PubMed:19561611, ECO:0000269|PubMed:20519495, ECO:0007744|PDB:2WFR, ECO:0007744|PDB:3N1G, ECO:0007744|PDB:3N1Q"; METAL 132; /note="Calcium 2"; /evidence="ECO:0000269|PubMed:19561611, ECO:0000269|PubMed:20519495, ECO:0007744|PDB:2WFR, ECO:0007744|PDB:3N1G, ECO:0007744|PDB:3N1Q"; METAL 141; /note="Zinc"; /evidence="ECO:0000269|PubMed:19561611, ECO:0000269|PubMed:20519495, ECO:0007744|PDB:2WFQ, ECO:0007744|PDB:2WFR, ECO:0007744|PDB:2WG3, ECO:0007744|PDB:3N1G, ECO:0007744|PDB:3N1Q"; METAL 148; /note="Zinc"; /evidence="ECO:0000269|PubMed:19561611, ECO:0000269|PubMed:20519495, ECO:0007744|PDB:2WFQ, ECO:0007744|PDB:2WFR, ECO:0007744|PDB:2WG3, ECO:0007744|PDB:3N1G, ECO:0007744|PDB:3N1Q"; METAL 183; /note="Zinc"; /evidence="ECO:0000269|PubMed:19561611, ECO:0000269|PubMed:20519495, ECO:0007744|PDB:2WFQ, ECO:0007744|PDB:2WFR, ECO:0007744|PDB:2WG3, ECO:0007744|PDB:3N1G, ECO:0007744|PDB:3N1Q" |
| Rhea ID | |
| Cross Reference Brenda |