| IED ID | IndEnz0002016424 |
| Enzyme Type ID | protease016424 |
| Protein Name |
Serine protease easter EC 3.4.21.- |
| Gene Name | ea CG4920 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MLKPSIICLFLGILAKSSAGQFYFPNEAAQVPNYGRCITPNRERALCIHLEDCKYLYGLLTTTPLRDTDRLYLSRSQCGYTNGKVLICCPDRYRESSSETTPPPKPNVTSNSLLPLPGQCGNILSNRIYGGMKTKIDEFPWMALIEYTKSQGKKGHHCGGSLISTRYVITASHCVNGKALPTDWRLSGVRLGEWDTNTNPDCEVDVRGMKDCAPPHLDVPVERTIPHPDYIPASKNQVNDIALLRLAQQVEYTDFVRPICLPLDVNLRSATFDGITMDVAGWGKTEQLSASNLKLKAAVEGSRMDECQNVYSSQDILLEDTQMCAGGKEGVDSCRGDSGGPLIGLDTNKVNTYYFLAGVVSFGPTPCGLAGWPGVYTLVGKYVDWIQNTIES |
| Enzyme Length | 392 |
| Uniprot Accession Number | P13582 |
| Absorption | |
| Active Site | ACT_SITE 173; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU00274; ACT_SITE 240; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU00274; ACT_SITE 338; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU00274 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Component of the extracellular signaling pathway that establishes the dorsal-ventral pathway of the embryo (PubMed:9477324, PubMed:2107028, PubMed:12493753). Three proteases; ndl, gd and snk process easter to create active easter (PubMed:9477324). Active easter defines cell identities along the dorsal-ventral continuum by activating the spz ligand for the Tl receptor in the ventral region of the embryo (PubMed:9477324). {ECO:0000269|PubMed:12493753, ECO:0000269|PubMed:2107028, ECO:0000269|PubMed:9477324}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (8); Domain (2); Metal binding (4); Mutagenesis (16); Propeptide (1); Sequence conflict (2); Signal peptide (1) |
| Keywords | Calcium;Developmental protein;Disulfide bond;Hydrolase;Metal-binding;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9477324}. |
| Modified Residue | |
| Post Translational Modification | PTM: Activation peptide and active catalytic domain are associated by a disulfide bond. Processed easter is present in extremely low amounts in the early embryo as it is rapidly converted into a high molecular mass complex, which may contain a protease inhibitor. Zymogen activation is also controlled by a negative feedback loop from Dorsal. |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000305 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10201075; 10449356; 10602463; 10696585; 10731134; 10793472; 10802234; 10961941; 10980426; 11133153; 11135568; 11283698; 11296245; 11350927; 11413042; 11493550; 11493599; 11514451; 11562344; 11700289; 11719206; 11742098; 11852243; 11901121; 12027434; 12098703; 12351681; 12364793; 12417410; 12421704; 12568721; 12642487; 1304399; 1339676; 1420309; 1425342; 14605208; 14654000; 14667416; 14711428; 14731500; 14731544; 15055573; 15082553; 15166158; 15199954; 15788537; 15840717; 16271864; 16322759; 16399077; 16537387; 1654049; 16566925; 1672778; 16861233; 1688006; 1733499; 1739964; 1742496; 17588928; 1765005; 17708965; 1794309; 17996400; 1814368; 1827421; 18477463; 18776742; 1904007; 19381251; 1965067; 2001838; 20220848; 20371351; 20605458; 20638387; 2068782; 20970335; 21074052; 2112301; 2185748; 22227521; 22570633; 22578419; 22611248; 22912810; 22949611; 23071443; 23369355; 23632253; 23729565; 23792537; 23868318; 2499514; 25000897; 25124754; 26453810; 26551273; 26843333; 2699854; 27058248; 27357258; 2891036; 29695493; 29935791; 30146479; 30367934; 31722958; 32143742; 32591083; 32901612; 33561221; 33827210; 3653004; 3931918; 6434989; 7475106; 7566149; 7705656; 7720559; 7859742; 7953570; 8001784; 8013912; 8026333; 8124709; 8139688; 8178370; 8223244; 8241766; 8241768; 8622655; 8698232; 8749385; 8808632; 8825480; 8909542; 8970732; 9258679; 9507077; 9550723; 9553105; 9598341; 9600835; 9676200; 9735365; 9827795; 9889092; |
| Motif | |
| Gene Encoded By | |
| Mass | 43,065 |
| Kinetics | |
| Metal Binding | METAL 193; /note=Calcium; /evidence=ECO:0000250|UniProtKB:O97366; METAL 195; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q9GRW0; METAL 198; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:O97366; METAL 201; /note=Calcium; /evidence=ECO:0000250|UniProtKB:O97366 |
| Rhea ID | |
| Cross Reference Brenda |