| IED ID | IndEnz0002016443 |
| Enzyme Type ID | protease016443 |
| Protein Name |
Probable enoyl-CoA hydratase 2, mitochondrial EC 4.2.1.17 |
| Gene Name | At4g16800 dl4425c |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MSFVKYLRRDNLLQLAGKPSLSRNYILQTCRTLIIETSPPEFVKLNRLSGSDSGIIEVNLDRPVTKNAINKEMLKSLQNAFESIHQDNSARVVMIRSLVPGVFCAGADLKERRTMSPSEVHTYVNSLRYMFSFIEALSIPTIAAIEGAALGGGLEMALACDLRICGENAVFGLPETGLAIIPGAGGTQRLSRLVGRSVSKELIFTGRKIDAIEAANKGLVNICVTAGEAHEKAIEMAQQINEKGPLAIKMAKKAIDEGIETNMASGLEVEEMCYQKLLNTQDRLEGLAAFAEKRKPLYTGN |
| Enzyme Length | 301 |
| Uniprot Accession Number | F4JML5 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 152; /note=Substrate; via amide nitrogen; /evidence=ECO:0000250|UniProtKB:P42126 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O; Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318, ChEBI:CHEBI:58856; EC=4.2.1.17; Evidence={ECO:0000305}; CATALYTIC ACTIVITY: Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O; Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521, ChEBI:CHEBI:137480; EC=4.2.1.17; Evidence={ECO:0000305}; |
| DNA Binding | |
| EC Number | 4.2.1.17 |
| Enzyme Function | FUNCTION: Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. {ECO:0000250|UniProtKB:P30084}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Lipid metabolism; fatty acid beta-oxidation. {ECO:0000305}. |
| nucleotide Binding | |
| Features | Binding site (1); Chain (1); Erroneous gene model prediction (2); Region (1); Site (1); Transit peptide (1) |
| Keywords | Fatty acid metabolism;Hydrolase;Lipid metabolism;Lyase;Mitochondrion;Protease;Reference proteome;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14576160; 15561727; 16040662; 17580301; 29742810; |
| Motif | |
| Gene Encoded By | |
| Mass | 32,788 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:16105; RHEA:20724 |
| Cross Reference Brenda | 4.2.1.18; |