Detail Information for IndEnz0002016448
IED ID IndEnz0002016448
Enzyme Type ID protease016448
Protein Name Caspase Dronc
EC 3.4.22.-
NEDD2-like caspase

Cleaved into: Caspase Nc subunit 1; Caspase Nc subunit 2
Gene Name Dronc Nc CG8091
Organism Drosophila melanogaster (Fruit fly)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly)
Enzyme Sequence MQPPELEIGMPKRHREHIRKNLNILVEWTNYERLAMECVQQGILTVQMLRNTQDLNGKPFNMDEKDVRVEQHRRLLLKITQRGPTAYNLLINALRNINCLDAAVLLESVDESDSRPPFISLNERRTSRKSADIVDTPSPEASEGPCVSKLRNEPLGALTPYVGVVDGPEVKKSKKIHGGDSAILGTYKMQSRFNRGVLLMVNIMDYPDQNRRRIGAEKDSKSLIHLFQELNFTIFPYGNVNQDQFFKLLTMVTSSSYVQNTECFVMVLMTHGNSVEGKEKVEFCDGSVVDMQKIKDHFQTAKCPYLVNKPKVLMFPFCRGDEYDLGHPKNQGNLMEPVYTAQEEKWPDTQTEGIPSPSTNVPSLADTLVCYANTPGYVTHRDLDTGSWYIQKFCQVMADHAHDTDLEDILKKTSEAVGNKRTKKGSMQTGAYDNLGFNKKLYFNPGFFNE
Enzyme Length 450
Uniprot Accession Number Q9XYF4
Absorption
Active Site ACT_SITE 271; /evidence=ECO:0000250|UniProtKB:P29466; ACT_SITE 318; /evidence=ECO:0000250|UniProtKB:P29466
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.22.-
Enzyme Function FUNCTION: Involved in the activation cascade of caspases responsible for apoptosis execution. Effector of steroid-mediated apoptosis during insect metamorphosis. Overexpression promotes programmed cell death. Interaction with Diap1 is required to suppress Dronc-mediated cell death; via Diap1-mediated ubiquitination of Dronc. Rate-limiting caspase in rpr and hid death pathway. {ECO:0000269|PubMed:10200258, ECO:0000269|PubMed:10675329, ECO:0000269|PubMed:10984473, ECO:0000269|PubMed:14517550}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Beta strand (10); Chain (2); Domain (1); Helix (7); Mutagenesis (1); Propeptide (2); Region (1); Turn (3)
Keywords 3D-structure;Apoptosis;Cytoplasm;Hydrolase;Protease;Reference proteome;Thiol protease;Ubl conjugation;Zymogen
Interact With Q7KLI1; Q24306; O01382
Induction INDUCTION: By ecdysone; exposure of salivary glands and midgut isolated from second instar larvae results in a massive increase in levels. {ECO:0000269|PubMed:10200258}.
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
Modified Residue
Post Translational Modification PTM: Ubiquitinated by Diap1, leading to its subsequent degradation. {ECO:0000269|PubMed:12021771}.
Signal Peptide
Structure 3D Electron microscopy (1); X-ray crystallography (2)
Cross Reference PDB 1Q4Q; 2FP3; 3J9K;
Mapped Pubmed ID 10481910; 10511707; 10559952; 10619023; 10639136; 10684245; 10704400; 10764741; 10825159; 10908589; 10934188; 10983989; 11048731; 11102824; 11139272; 11139274; 11139276; 11139278; 11139280; 11165519; 11178240; 11237461; 11262243; 11287192; 11337486; 11494315; 11550090; 11901173; 11997504; 12006672; 12011068; 12021767; 12021769; 12045184; 12065414; 12070100; 12072176; 12084706; 12095613; 12110175; 12176339; 12297099; 12356728; 12397080; 12441239; 12453466; 12473344; 12478468; 12502734; 12547518; 12593803; 12593804; 12692559; 12725740; 12729588; 12737795; 12737804; 12783893; 12814564; 12850443; 12852852; 12853472; 12906812; 12938178; 12970673; 1450511; 14532134; 14576353; 14644196; 14647296; 14657035; 14668412; 14724249; 14737191; 14744432; 14764878; 14976192; 14980217; 14996492; 15082313; 15107838; 15173191; 15239946; 15242648; 15268856; 15337122; 15355784; 15383617; 15469838; 15522457; 15533997; 15572131; 15572132; 15573123; 15640802; 15657059; 15704171; 15710747; 15774476; 15776001; 15782175; 15800001; 15860727; 15900708; 15922568; 15970694; 16222340; 16224045; 16303568; 16314564; 16322754; 16324723; 16362035; 16446367; 16456582; 16485033; 16528381; 16533943; 16595176; 16612543; 16645642; 16672345; 16797177; 16816412; 16880123; 16887178; 16887350; 16887831; 16890515; 16901779; 16906130; 16920621; 16954388; 16980627; 16980964; 17000765; 17027478; 17068333; 17183368; 17183370; 17205079; 17256009; 17275304; 17310982; 17339382; 17341581; 17347664; 17464325; 17522156; 17522160; 17557079; 17582002; 17591924; 17612403; 17636001; 17679695; 17682052; 17728345; 17937502; 17988176; 17989181; 17998402; 18039869; 18045837; 18083103; 18084239; 18309328; 18331718; 18367553; 18455715; 18506601; 18695041; 18794330; 19011620; 19026784; 19064709; 19077536; 19084594; 19100727; 19182545; 19214204; 19217892; 19224859; 19244279; 19330035; 19364807; 19375319; 19428445; 19465595; 19483676; 19497884; 19498442; 19720858; 19737742; 19744473; 19818615; 19822670; 19855017; 19874832; 19960024; 19960025; 20014406; 20016284; 20023159; 20023160; 20063412; 20100334; 20122400; 20127699; 20220848; 20445064; 20478529; 20624856; 20627310; 20679206; 20682638; 20700104; 20882681; 20951343; 21035895; 21074052; 21113144; 21120926; 21136153; 21179433; 21220123; 21338340; 21338343; 21343367; 21385866; 21425087; 21471375; 21558368; 21811237; 21844890; 21886178; 21886179; 21909282; 21958154; 22036477; 22065747; 22095281; 22153373; 22238688; 22351928; 22367497; 22393355; 22479597; 22539005; 22555454; 22898807; 22900232; 22981648; 23018595; 23028118; 23071443; 23087837; 23184991; 23209440; 23222440; 23264732; 23306653; 23336071; 23408884; 23447678; 23519152; 23523076; 23561633; 23644660; 23722427; 23743202; 23754750; 23784845; 23810935; 23913920; 23940367; 23943356; 23951408; 23968895; 24023255; 24036278; 24066226; 24096487; 24239163; 24316411; 24333635; 24336022; 24362437; 24374353; 24492611; 24497843; 24553922; 24598157; 24643212; 24685612; 24746817; 24793180; 24819984; 24865556; 24891502; 24907259; 24967585; 24981286; 25012819; 25015062; 25036253; 25146930; 25171415; 25217223; 25255315; 25312911; 25329560; 25332163; 25398939; 25579975; 25613379; 25644603; 25668151; 25790850; 25855961; 25868821; 25933381; 25951229; 25963257; 25968311; 25968339; 26010168; 26021298; 26034307; 26069889; 26134911; 26160069; 26255846; 26291171; 26362609; 26374522; 26413413; 26647185; 26896675; 26898463; 26900934; 26912791; 26960254; 26972874; 27058168; 27104928; 27172210; 27177021; 27256879; 27308545; 27351747; 27518434; 27537218; 27558818; 27575697; 27773719; 27923046; 27999411; 28045022; 28068320; 28077877; 28207763; 28211869; 28401354; 28430154; 28455414; 28492538; 28514643; 28524858; 28619822; 28631994; 28683272; 28688927; 28751499; 28853394; 28870988; 28893998; 28900135; 28928435; 28935711; 28945745; 29033939; 29050293; 29084807; 29108021; 29372681; 29385168; 29431244; 29447906; 29576455; 29670104; 29750169; 29764857; 29765111; 29787709; 29854765; 29870691; 29970915; 30022065; 30036358; 30080872; 30159848; 30413561; 30462636; 30563404; 30741974; 30770378; 30796611; 30836847; 30855229; 30872455; 30901919; 30904193; 30912203; 30919912; 31068592; 31136986; 31231648; 31255773; 31409797; 31438818; 31548372; 31566767; 31641108; 31722958; 31745213; 31772150; 31822470; 31909714; 32053595; 32157127; 32174999; 32287290; 32658991; 32658993; 32686670; 32698005; 32707222; 32791328; 32796885; 32845992; 32894223; 33022230; 33063715; 33075096; 33135280; 33184261; 33238125; 33309868; 33377870; 33507966; 33514791; 33561221; 33662357; 33802079; 33863891; 33920158; 33983927; 34081909; 34120097; 34313318; 34349099; 34463269;
Motif
Gene Encoded By
Mass 51,141
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda