| IED ID | IndEnz0002016451 |
| Enzyme Type ID | protease016451 |
| Protein Name |
Endothelin-converting enzyme 1 ECE-1 EC 3.4.24.71 |
| Gene Name | ECE1 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MRGVWPPPVSALLSALGMSTYKRATLDEEDLVDSLSEGDAYPNGLQVNFHSPRSGQRCWAARTQVEKRLVVLVVLLAAGLVACLAALGIQYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW |
| Enzyme Length | 770 |
| Uniprot Accession Number | P42892 |
| Absorption | |
| Active Site | ACT_SITE 608; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000255|PROSITE-ProRule:PRU10095, ECO:0000305|PubMed:18992253"; ACT_SITE 671; /note="Proton donor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000305|PubMed:18992253" |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by phosphoramidon (PubMed:18992253). Activated by K49-P1-20, a twenty-residue synthetic peptide shortened from the snake B.asper myotoxin II (PubMed:26931059). {ECO:0000269|PubMed:26931059}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of the 21-Trp-|-Val-22 bond in big endothelin to form endothelin 1.; EC=3.4.24.71; |
| DNA Binding | |
| EC Number | 3.4.24.71 |
| Enzyme Function | FUNCTION: Converts big endothelin-1 to endothelin-1. {ECO:0000269|PubMed:9396733}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (3); Beta strand (12); Chain (1); Disulfide bond (5); Domain (1); Erroneous gene model prediction (1); Erroneous initiation (1); Glycosylation (10); Helix (34); Metal binding (3); Modified residue (1); Mutagenesis (1); Natural variant (2); Topological domain (2); Transmembrane (1); Turn (6) |
| Keywords | 3D-structure;Alternative splicing;Cell membrane;Disease variant;Disulfide bond;Glycoprotein;Hirschsprung disease;Hydrolase;Membrane;Metal-binding;Metalloprotease;Phosphoprotein;Protease;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix;Zinc |
| Interact With | P49760; A8MQ03; P60370; P60410 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. |
| Modified Residue | MOD_RES 25; /note=Phosphothreonine; /evidence=ECO:0007744|PubMed:19690332 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 3DWB; |
| Mapped Pubmed ID | 10438732; 11723113; 11906289; 12011762; 12244060; 12393864; 12464614; 12609744; 14597855; 15010576; 15126915; 15240857; 15340356; 15665524; 16023075; 16234608; 16526315; 16531800; 16567585; 16698938; 16986361; 17264805; 17545092; 17592116; 17618613; 17664854; 17701914; 17712175; 17761169; 17977716; 18039931; 18334739; 18660489; 18781169; 18974277; 19222484; 19289136; 19531493; 19889475; 19913121; 20037208; 20217867; 20345647; 20346360; 20485444; 20538960; 20558134; 20628086; 20663017; 20711500; 21554123; 21777246; 21851036; 22027013; 22322595; 22416137; 22693153; 22728136; 22731820; 22972025; 23022525; 23175834; 23283972; 23360525; 23816989; 23863409; 24000822; 24026618; 24497914; 24595843; 24812665; 24898255; 25226840; 25268585; 25416956; 25469848; 25501500; 26514267; 26543229; 26806547; 27036146; 28171705; 29978582; 30626614; 30926432; 31777301; 31788944; 32107880; 32170981; 32278308; 8062389; 9595387; |
| Motif | |
| Gene Encoded By | |
| Mass | 87,164 |
| Kinetics | |
| Metal Binding | METAL 607; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000269|PubMed:18992253"; METAL 611; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000269|PubMed:18992253"; METAL 667; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000269|PubMed:18992253" |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.71; |