| IED ID | IndEnz0002016452 |
| Enzyme Type ID | protease016452 |
| Protein Name |
Endothelin-converting enzyme 1 ECE-1 EC 3.4.24.71 |
| Gene Name | Ece1 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MRTVWSPLAAALAALGMSTYKRATLDEEDLVDSLSEGDVYPNGLQVNFRSSRSGQRCWAARTSVEKRLVVLVTLLAAGLVACLAALGIQYQTRTPPVCLTEACVSVTSSILNSMDPTVDPCQDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAVIKHLLENATASVSEAERKAQVYYRACMNETRIEELRAKPLMELIEKLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEDAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLRQVSTLINNTDKCLLNNYMMWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCIPRWKFCVSDTENNLGFALGPMFVKATFAEDSKNIASEIIMEIKKAFEESLSTLKWMDEETRRSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTECMVQQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTSNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPHHKCEVW |
| Enzyme Length | 769 |
| Uniprot Accession Number | Q4PZA2 |
| Absorption | |
| Active Site | ACT_SITE 607; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000255|PROSITE-ProRule:PRU10095"; ACT_SITE 670; /note="Proton donor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233" |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by phosphoramidon. {ECO:0000250}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of the 21-Trp-|-Val-22 bond in big endothelin to form endothelin 1.; EC=3.4.24.71; |
| DNA Binding | |
| EC Number | 3.4.24.71 |
| Enzyme Function | FUNCTION: Converts big endothelin-1 to endothelin-1. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (3); Chain (1); Disulfide bond (5); Domain (1); Glycosylation (10); Metal binding (3); Modified residue (1); Topological domain (2); Transmembrane (1) |
| Keywords | Alternative splicing;Cell membrane;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Phosphoprotein;Protease;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}. |
| Modified Residue | MOD_RES 24; /note=Phosphothreonine; /evidence=ECO:0000250|UniProtKB:P42892 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10626068; 10811845; 11290299; 11328956; 12464614; 12520002; 12950083; 14610273; 14976138; 15226823; 16110338; 16602821; 16698938; 16912050; 17131407; 17967808; 18385664; 18401410; 18665160; 18772340; 18799693; 18973680; 18997155; 19261723; 19531493; 20802183; 21193583; 21267068; 21677750; 21878523; 22972025; 23306833; 23699715; 24586749; 24812665; 24875861; 25181417; 26849124; 27370713; 27644077; 28109039; 28409408; 29524599; 30735130; 32768584; 8062389; 8548579; 9449665; 9649553; |
| Motif | |
| Gene Encoded By | |
| Mass | 87,073 |
| Kinetics | |
| Metal Binding | METAL 606; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000255|PROSITE-ProRule:PRU10095"; METAL 610; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233, ECO:0000255|PROSITE-ProRule:PRU10095"; METAL 666; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01233" |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.71; |