| IED ID | IndEnz0002016522 |
| Enzyme Type ID | protease016522 |
| Protein Name |
Rhomboid protease AarA EC 3.4.21.105 Intramembrane serine protease |
| Gene Name | aarA |
| Organism | Providencia stuartii |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Morganellaceae Providencia Providencia stuartii |
| Enzyme Sequence | MAEQQNPFSIKSKARFSLGAIALTLTLVLLNIAVYFYQIVFASPLDSRESNLILFGANIYQLSLTGDWWRYPISMMLHSNGTHLAFNCLALFVIGIGCERAYGKFKLLAIYIISGIGAALFSAYWQYYEISNSDLWTDSTVYITIGVGASGAIMGIAAASVIYLIKVVINKPNPHPVIQRRQKYQLYNLIAMIALTLINGLQSGVDNAAHIGGAIIGALISIAYILVPHKLRVANLCITVIAASLLTMMIYLYSFSTNKHLLEEREFIYQEVYTELADANQ |
| Enzyme Length | 281 |
| Uniprot Accession Number | P46116 |
| Absorption | |
| Active Site | ACT_SITE 150; /note=Nucleophile; /evidence=ECO:0000305; ACT_SITE 210; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.; EC=3.4.21.105; Evidence={ECO:0000269|PubMed:21439629}; |
| DNA Binding | |
| EC Number | 3.4.21.105 |
| Enzyme Function | FUNCTION: Rhomboid serine protease that catalyzes intramembrane proteolysis. Mediates quorum-sensing and the subsequent regulation of target genes via activation of the Tat protein export system. Catalyzes the proteolytic activation of TatA by removal of its N-terminal extension. {ECO:0000269|PubMed:15616571, ECO:0000269|PubMed:17215357, ECO:0000269|PubMed:8051030}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Mutagenesis (2); Transmembrane (7) |
| Keywords | Cell membrane;Hydrolase;Membrane;Protease;Serine protease;Transmembrane;Transmembrane helix |
| Interact With | A1YH68 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15616571, ECO:0000269|PubMed:17215357}; Multi-pass membrane protein {ECO:0000269|PubMed:15616571, ECO:0000269|PubMed:17215357}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 25009246; |
| Motif | |
| Gene Encoded By | |
| Mass | 31,106 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.105; |