| IED ID | IndEnz0002016526 |
| Enzyme Type ID | protease016526 |
| Protein Name |
Angiotensin-converting enzyme-related protein EC 3.4.15.1 |
| Gene Name | Acer CG10593 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MGACNITVLLLVIMLWLPHGLSMGNSCSASVLEARRFFELENEQLRRRFHEEFLSGYNYNTNVTEANRQAMIEVYARNAELNKRLAQQIKSSDYVQSEDADIRRQAEHLSKLGASALNADDYLALQNAISSMQTNYATATVCSYTNRSDCSLTLEPHIQERLSHSRDPAELAWYWREWHDKSGTPMRQNFAEYVRLTRKASQLNGHRSYADYWVQFYEDPDFERQLDATFKQLLPFYRQLHGYVRFRLRQHYGPDVMPAEGNIPISLLGNMWGQSWNELLDLFTPYPEKPFVDVKAEMEKQGYTVQKLFELGDQFFQSLGMRALPPSFWNLSVLTRPDDRHVVCHASAWDFYQDSDVRIKMCTEVDSHYFYVVHHELGHIQYYLQYEQQPAVYRGAPNPGFHEAVGDVIALSVMSAKHLKAIGLIENGRLDEKSRINQLFKQALSKIVFLPFGYAVDKYRYAVFRNELDESQWNCGFWQMRSEFGGVEPPVFRTEKDFDPPAKYHIDADVEYLRYFAAHIFQFQFHKALCRKAGQYAPNNSRLTLDNCDIFGSKAAGRSLSQFLSKGNSRHWKEVLEEFTGETEMDPAALLEYFEPLYQWLKQENSRLGVPLGWGPTDKIPSDCCGTFST |
| Enzyme Length | 630 |
| Uniprot Accession Number | Q9VLJ6 |
| Absorption | |
| Active Site | ACT_SITE 376; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by captopril, lisinopril, trandolaprilat, fosinoprilat and enalaprilat. {ECO:0000269|PubMed:9839949}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.; EC=3.4.15.1; |
| DNA Binding | |
| EC Number | 3.4.15.1 |
| Enzyme Function | FUNCTION: May be involved in the specific maturation or degradation of a number of bioactive peptides. May have a role in the specification of heart progenitors. {ECO:0000269|PubMed:12075344}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.6. {ECO:0000269|PubMed:9839949}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (3); Metal binding (3); Sequence conflict (3); Signal peptide (1) |
| Keywords | Carboxypeptidase;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space {ECO:0000269|PubMed:9839949}. |
| Modified Residue | |
| Post Translational Modification | PTM: Glycosylated. {ECO:0000269|PubMed:9839949}. |
| Signal Peptide | SIGNAL 1..22; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10471706; 10471707; 10913309; 10961940; 10961941; 11733057; 12431739; 12431741; 14605208; 15458575; 16403023; 17849284; 17921161; 18188155; 18573871; 20220848; 20371351; 20951134; 21074052; 21270318; 21480662; 22375135; 23071443; 24002232; 25236355; 25294943; 25409104; 25687947; 25887180; 26370122; 27226164; 27289019; 27794539; 27894253; 30940674; 31722958; 31786681; 32989002; 33060184; 33100975; 9409773; |
| Motif | |
| Gene Encoded By | |
| Mass | 73,057 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1.2 mM for Hip-His-Leu {ECO:0000269|PubMed:9839949}; KM=4.35 mM for Hip-His-Leu-NH(2) {ECO:0000269|PubMed:9839949}; KM=40.6 uM for (Leu5)enkephalin {ECO:0000269|PubMed:9839949}; KM=949 uM for (Leu5)enkephalinamide {ECO:0000269|PubMed:9839949}; |
| Metal Binding | METAL 375; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 379; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 403; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Rhea ID | |
| Cross Reference Brenda |