| IED ID | IndEnz0002016551 |
| Enzyme Type ID | protease016551 |
| Protein Name |
Disintegrin and metalloproteinase domain-containing protein 12 ADAM 12 EC 3.4.24.- Meltrin-alpha |
| Gene Name | Adam12 Mltna |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MAERPARRAPPARALLLALAGALLAPRAARGMSLWDQRGTYEVARASLLSKDPGIPGQSIPAKDHPDVLTVQLQLESRDLILSLERNEGLIANGFTETHYLQDGTDVSLTRNHTDHCYYHGHVQGDAASVVSLSTCSGLRGLIMFENKTYSLEPMKNTTDSYKLVPAESMTNIQGLCGSQHNKSNLTMEDVSPGTSQMRARRHKRETLKMTKYVELVIVADNREFQRQGKDLEKVKQRLIEIANHVDKFYRPLNIRIVLVGVEVWNDIDKCSISQDPFTSLHEFLDWRKIKLLPRKSHDNAQLISGVYFQGTTIGMAPIMSMCTAEQSGGVVMDHSDSPLGAAVTLAHELGHNFGMNHDTLERGCSCRMAAEKGGCIMNPSTGFPFPMVFSSCSRKDLEASLEKGMGMCLFNLPEVKQAFGGRKCGNGYVEEGEECDCGEPEECTNRCCNATTCTLKPDAVCAHGQCCEDCQLKPPGTACRGSSNSCDLPEFCTGTAPHCPANVYLHDGHPCQGVDGYCYNGICQTHEQQCVTLWGPGAKPAPGICFERVNSAGDPYGNCGKDSKSAFAKCELRDAKCGKIQCQGGASRPVIGTNAVSIETNIPQQEGGRILCRGTHVYLGDDMPDPGLVLAGTKCAEGKICLNRRCQNISVFGVHKCAMQCHGRGVCNNRKNCHCEAHWAPPFCDKFGFGGSTDSGPIRQADNQGLTVGILVSILCLLAAGFVVYLKRKTLMRLLFTHKKTTMEKLRCVHPSRTPSGPHLGQAHHTPGKGLLMNRAPHFNTPKDRHSLKCQNMDISRPLDARAVPQLQSPQRVLLPLHQTPRAPSGPARPLPASPAVRQAQGIRKPSPPQKPLPADPLSRTSRLTSALVRTPGQQEPGHRPAPIRPAPKHQVPRPSHNAYIK |
| Enzyme Length | 903 |
| Uniprot Accession Number | Q61824 |
| Absorption | |
| Active Site | ACT_SITE 349; /evidence="ECO:0000255|PROSITE-ProRule:PRU00276, ECO:0000255|PROSITE-ProRule:PRU10095" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (7); Domain (3); Glycosylation (7); Metal binding (4); Modified residue (1); Motif (6); Propeptide (1); Region (2); Sequence conflict (3); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | Cell adhesion;Cleavage on pair of basic residues;Disulfide bond;EGF-like domain;Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Phosphoprotein;Protease;Reference proteome;SH3-binding;Signal;Transmembrane;Transmembrane helix;Zinc;Zymogen |
| Interact With | P35609 |
| Induction | INDUCTION: At the onset of myoblast fusion. |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. |
| Modified Residue | MOD_RES 901; /note=Phosphotyrosine; by SRC; /evidence=ECO:0000305|PubMed:11127814 |
| Post Translational Modification | PTM: The precursor is cleaved by a furin endopeptidase. {ECO:0000250}. |
| Signal Peptide | SIGNAL 1..31; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10527948; 12000741; 12000744; 12414501; 12466851; 12482960; 12615925; 12915458; 12972593; 14993236; 15063736; 15637293; 15849365; 15907280; 15930294; 15936750; 16054021; 16079176; 16141072; 16602821; 16607276; 16869727; 16916601; 17072319; 17107962; 17344430; 17620406; 17653278; 17982130; 18241035; 19398663; 19581512; 19841944; 20457602; 20660068; 21062905; 21267068; 21491542; 21875931; 23458101; 23686306; 24006456; 24103556; 24336287; 24798404; 25892053; 26163448; 26242473; 26975138; 27487498; 29846135; 30042416; 30389854; 30753595; 30993375; 31774689; 32913205; 33952697; 9461614; |
| Motif | MOTIF 175..182; /note=Cysteine switch; /evidence=ECO:0000250; MOTIF 824..830; /note=SH3-binding; class II; MOTIF 830..837; /note=SH3-binding; class I; MOTIF 846..852; /note=SH3-binding; class II; MOTIF 852..858; /note=SH3-binding; class I; MOTIF 881..887; /note=SH3-binding; class I |
| Gene Encoded By | |
| Mass | 98,504 |
| Kinetics | |
| Metal Binding | METAL 177; /note=Zinc; in inhibited form; /evidence=ECO:0000250; METAL 348; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 352; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 358; /note=Zinc; catalytic; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.B10; |