| IED ID | IndEnz0002016589 |
| Enzyme Type ID | protease016589 |
| Protein Name |
Cytosolic carboxypeptidase 4 EC 3.4.17.- EC 3.4.17.24 ATP/GTP-binding protein-like 1 Protein deglutamylase CCP4 |
| Gene Name | Agbl1 Ccp4 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MAEQEGSGLQMLLHTLQNSSDKASTLSILQVLGDLLSVGTDRRIYYMISKGGSEALLQTLVDTARSSSPDWDILLPLFRLLAKVGLRDKKFGQKALELEALDVTLILARKNLSHSQNLLHCLWVLRVFASSVTTGAMLGINGAMELLFKVLSPYTRKHTRTIRAATEVLAALLKSSKHLRRAVNRGYVNSLLRLHQDWHSRDVTNTYVTIRHGLLLCLRHIVALRSGREAFLAAQGMETLFSSAQTCLENKNMELVISAVIQILRQCYPASRLPLVTASSAYTFPAPGSTSSELPLNLTEEDFDDDGDEEMDKDSDVEAVKEDDDLETDLSKLSSKPGLDLPEEELAQYDAMCPELSCSFEELEPKCGDDLNNKDTLHANHHHIPSVASLRQHCFNREHSSWRQEREDTVHSSILHMVKTGKSGVPSSSKQRSATNVNQSLQQNGLEIDSSGHDTSDIQAPLEQAAWDMEAISCPRITASFPNSTKPEESIGAAEKLLHTHAKHIPFHDPHLYIANAMRTRSAVGFKTMAFPDLWGHCPPPAAQPMLDRKLGVQRIKILEDIRRLLHPSDVINKVVFSLDEPRPLQGSISNCLMFHSKFESGNLRKAIQVREFEYDLLVNADVNSSQHQQWFYFKVSGMRAAVPYHFNIINCEKPNSQFNYGMQPTLYSVKEALLGRPAWIRTGSDICYYKNHYRQNAATMDGALGKRYYTLTFAVTFPHNEDACYLAYHYPYTYSTLMTHLEILERSIDHREIYFRHDVLCQTLGGNPCPLVTITAFPESNSTEHLEQFRCRPYQVITARVHPGESNASWVMKGTLEFLVSSDPVAKLLRENFVFKIIPMLNPDGVINGNHRCSLRGEDLNRQWLSPQAHLQPTIYHAKGLLHYLSSTGRGPVVFCDFHGHSQKKNVFLYGCSMKETLWQAGCTVGESALLEDVSYRTLPKILDKLAPAFTMNSCSFLVEKSRASTARVVVWREMGVSRSYTMESSYCGCNQGPYQGLQFGTGELEEMGAMYCLGLLILELKSVNCSHKLLARASSLLNADVLEHYLQRCSSSSSNSSNRTSEVDDEPYCMEEIDYSADSSSDAEQNFTELDRQIQECALNKDEEEEEKEEGTGWRRRSVT |
| Enzyme Length | 1122 |
| Uniprot Accession Number | Q09M05 |
| Absorption | |
| Active Site | ACT_SITE 853; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P00730 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(L-glutamyl)(n+1)-gamma-L-glutamyl-L-glutamyl-[protein] + H2O = (L-glutamyl)(n)-gamma-L-glutamyl-L-glutamyl-[protein] + L-glutamate; Xref=Rhea:RHEA:60004, Rhea:RHEA-COMP:15519, Rhea:RHEA-COMP:15675, ChEBI:CHEBI:15377, ChEBI:CHEBI:29985, ChEBI:CHEBI:143623; Evidence={ECO:0000269|PubMed:21074048};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60005; Evidence={ECO:0000305|PubMed:21074048}; CATALYTIC ACTIVITY: Reaction=C-terminal L-alpha-aminoacyl-L-glutamyl-L-glutamyl-[tubulin] + H2O = C-terminal L-alpha-aminoacyl-L-glutamyl-[tubulin] + L-glutamate; Xref=Rhea:RHEA:63792, Rhea:RHEA-COMP:16435, Rhea:RHEA-COMP:16436, ChEBI:CHEBI:15377, ChEBI:CHEBI:29985, ChEBI:CHEBI:149555, ChEBI:CHEBI:149556; EC=3.4.17.24; Evidence={ECO:0000269|PubMed:21074048};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63793; Evidence={ECO:0000305|PubMed:21074048}; |
| DNA Binding | |
| EC Number | 3.4.17.-; 3.4.17.24 |
| Enzyme Function | FUNCTION: Metallocarboxypeptidase that mediates deglutamylation of tubulin and non-tubulin target proteins (PubMed:21074048). Catalyzes the removal of polyglutamate side chains present on the gamma-carboxyl group of glutamate residues within the C-terminal tail of tubulin protein (PubMed:21074048). Specifically cleaves tubulin long-side-chains, while it is not able to remove the branching point glutamate (PubMed:21074048). Also catalyzes the removal of polyglutamate residues from the carboxy-terminus of non-tubulin proteins such as MYLK (PubMed:21074048). {ECO:0000269|PubMed:21074048}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Alternative sequence (5); Chain (1); Compositional bias (1); Metal binding (3); Mutagenesis (2); Region (2); Sequence conflict (2) |
| Keywords | Alternative splicing;Carboxypeptidase;Cytoplasm;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q96MI9}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 16602821; 16952463; 21267068; 26829768; 28794449; 29593216; 31541165; |
| Motif | |
| Gene Encoded By | |
| Mass | 126,149 |
| Kinetics | |
| Metal Binding | METAL 803; /note=Zinc; /evidence=ECO:0000305; METAL 806; /note=Zinc; /evidence=ECO:0000305; METAL 900; /note=Zinc; /evidence=ECO:0000250|UniProtKB:P00730 |
| Rhea ID | RHEA:60004; RHEA:60005; RHEA:63792; RHEA:63793 |
| Cross Reference Brenda |