Detail Information for IndEnz0002016643
IED ID IndEnz0002016643
Enzyme Type ID protease016643
Protein Name Calpain-9
EC 3.4.22.-
Digestive tract-specific calpain
New calpain 4
nCL-4
Protein CG36
Gene Name CAPN9 NCL4
Organism Homo sapiens (Human)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human)
Enzyme Sequence MPYLYRAPGPQAHPVPKDARITHSSGQSFEQMRQECLQRGTLFEDADFPASNSSLFYSERPQIPFVWKRPGEIVKNPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALARVIPQDQSFGPGYAGIFHFQFWQHSEWLDVVIDDRLPTFRDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAETFQTKEAPENFYEILEKALKRGSLLGCFIDTRSAAESEARTPFGLIKGHAYSVTGIDQVSFRGQRIELIRIRNPWGQVEWNGSWSDSSPEWRSVGPAEQKRLCHTALDDGEFWMAFKDFKAHFDKVEICNLTPDALEEDAIHKWEVTVHQGSWVRGSTAGGCRNFLDTFWTNPQIKLSLTEKDEGQEECSFLVALMQKDRRKLKRFGANVLTIGYAIYECPDKDEHLNKDFFRYHASRARSKTFINLREVSDRFKLPPGEYILIPSTFEPHQEADFCLRIFSEKKAITRDMDGNVDIDLPEPPKPTPPDQETEEEQRFRALFEQVAGEDMEVTAEELEYVLNAVLQKKKDIKFKKLSLISCKNIISLMDTSGNGKLEFDEFKVFWDKLKQWINLFLRFDADKSGTMSTYELRTALKAAGFQLSSHLLQLIVLRYADEELQLDFDDFLNCLVRLENASRVFQALSTKNKEFIHLNINEFIHLTMNI
Enzyme Length 690
Uniprot Accession Number O14815
Absorption
Active Site ACT_SITE 97; /evidence=ECO:0000269|PubMed:17157313; ACT_SITE 254; /evidence=ECO:0000269|PubMed:17157313; ACT_SITE 278; /evidence=ECO:0000269|PubMed:17157313
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.22.-
Enzyme Function FUNCTION: Calcium-regulated non-lysosomal thiol-protease.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Alternative sequence (1); Beta strand (16); Chain (1); Domain (4); Helix (15); Metal binding (19); Natural variant (12); Region (4)
Keywords 3D-structure;Alternative splicing;Calcium;Hydrolase;Metal-binding;Protease;Reference proteome;Repeat;Thiol protease
Interact With
Induction INDUCTION: Down-regulated in gastric cancer tissue and in gastric cell lines of differentiated and poorly differentiated types.
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (2)
Cross Reference PDB 1ZIV; 2P0R;
Mapped Pubmed ID 12843408; 17608959; 19074885; 20379614; 20453000; 21030783; 21670566; 21864727; 23035980; 25539577; 27404891; 31316008;
Motif
Gene Encoded By
Mass 79,097
Kinetics
Metal Binding METAL 81; /note=Calcium 1; via carbonyl oxygen; METAL 83; /note=Calcium 1; via carbonyl oxygen; METAL 88; /note=Calcium 1; METAL 167; /note=Calcium 1; METAL 284; /note=Calcium 2; METAL 291; /note=Calcium 2; METAL 312; /note=Calcium 2; via carbonyl oxygen; METAL 314; /note=Calcium 2; METAL 316; /note=Calcium 2; via carbonyl oxygen; METAL 574; /note=Calcium 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 576; /note=Calcium 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 578; /note=Calcium 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 580; /note=Calcium 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 585; /note=Calcium 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 604; /note=Calcium 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 606; /note=Calcium 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 608; /note=Calcium 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 610; /note=Calcium 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 615; /note=Calcium 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448
Rhea ID
Cross Reference Brenda 3.4.22.B28;3.4.22.B29;