| IED ID | IndEnz0002016643 |
| Enzyme Type ID | protease016643 |
| Protein Name |
Calpain-9 EC 3.4.22.- Digestive tract-specific calpain New calpain 4 nCL-4 Protein CG36 |
| Gene Name | CAPN9 NCL4 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MPYLYRAPGPQAHPVPKDARITHSSGQSFEQMRQECLQRGTLFEDADFPASNSSLFYSERPQIPFVWKRPGEIVKNPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALARVIPQDQSFGPGYAGIFHFQFWQHSEWLDVVIDDRLPTFRDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAETFQTKEAPENFYEILEKALKRGSLLGCFIDTRSAAESEARTPFGLIKGHAYSVTGIDQVSFRGQRIELIRIRNPWGQVEWNGSWSDSSPEWRSVGPAEQKRLCHTALDDGEFWMAFKDFKAHFDKVEICNLTPDALEEDAIHKWEVTVHQGSWVRGSTAGGCRNFLDTFWTNPQIKLSLTEKDEGQEECSFLVALMQKDRRKLKRFGANVLTIGYAIYECPDKDEHLNKDFFRYHASRARSKTFINLREVSDRFKLPPGEYILIPSTFEPHQEADFCLRIFSEKKAITRDMDGNVDIDLPEPPKPTPPDQETEEEQRFRALFEQVAGEDMEVTAEELEYVLNAVLQKKKDIKFKKLSLISCKNIISLMDTSGNGKLEFDEFKVFWDKLKQWINLFLRFDADKSGTMSTYELRTALKAAGFQLSSHLLQLIVLRYADEELQLDFDDFLNCLVRLENASRVFQALSTKNKEFIHLNINEFIHLTMNI |
| Enzyme Length | 690 |
| Uniprot Accession Number | O14815 |
| Absorption | |
| Active Site | ACT_SITE 97; /evidence=ECO:0000269|PubMed:17157313; ACT_SITE 254; /evidence=ECO:0000269|PubMed:17157313; ACT_SITE 278; /evidence=ECO:0000269|PubMed:17157313 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Calcium-regulated non-lysosomal thiol-protease. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (1); Beta strand (16); Chain (1); Domain (4); Helix (15); Metal binding (19); Natural variant (12); Region (4) |
| Keywords | 3D-structure;Alternative splicing;Calcium;Hydrolase;Metal-binding;Protease;Reference proteome;Repeat;Thiol protease |
| Interact With | |
| Induction | INDUCTION: Down-regulated in gastric cancer tissue and in gastric cell lines of differentiated and poorly differentiated types. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 1ZIV; 2P0R; |
| Mapped Pubmed ID | 12843408; 17608959; 19074885; 20379614; 20453000; 21030783; 21670566; 21864727; 23035980; 25539577; 27404891; 31316008; |
| Motif | |
| Gene Encoded By | |
| Mass | 79,097 |
| Kinetics | |
| Metal Binding | METAL 81; /note=Calcium 1; via carbonyl oxygen; METAL 83; /note=Calcium 1; via carbonyl oxygen; METAL 88; /note=Calcium 1; METAL 167; /note=Calcium 1; METAL 284; /note=Calcium 2; METAL 291; /note=Calcium 2; METAL 312; /note=Calcium 2; via carbonyl oxygen; METAL 314; /note=Calcium 2; METAL 316; /note=Calcium 2; via carbonyl oxygen; METAL 574; /note=Calcium 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 576; /note=Calcium 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 578; /note=Calcium 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 580; /note=Calcium 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 585; /note=Calcium 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 604; /note=Calcium 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 606; /note=Calcium 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 608; /note=Calcium 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 610; /note=Calcium 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 615; /note=Calcium 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.22.B28;3.4.22.B29; |