| IED ID | IndEnz0002016649 |
| Enzyme Type ID | protease016649 |
| Protein Name |
DNA methyltransferase CcrM M.CcrMI EC 2.1.1.72 DNA adenine methyltransferase CcrM Type II methyltransferase M.CcrMI |
| Gene Name | ccrMIM ccrM CC_0378 |
| Organism | Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Alphaproteobacteria Caulobacterales Caulobacteraceae Caulobacter Caulobacter vibrioides (Caulobacter crescentus) Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus) |
| Enzyme Sequence | MKFGPETIIHGDCIEQMNALPEKSVDLIFADPPYNLQLGGDLLRPDNSKVDAVDDHWDQFESFAAYDKFTREWLKAARRVLKDDGAIWVIGSYHNIFRVGVAVQDLGFWILNDIVWRKSNPMPNFKGTRFANAHETLIWASKSQNAKRYTFNYDALKMANDEVQMRSDWTIPLCTGEERIKGADGQKAHPTQKPEALLYRVILSTTKPGDVILDPFFGVGTTGAAAKRLGRKFIGIEREAEYLEHAKARIAKVVPIAPEDLDVMGSKRAEPRVPFGTIVEAGLLSPGDTLYCSKGTHVAKVRPDGSITVGDLSGSIHKIGALVQSAPACNGWTYWHFKTDAGLAPIDVLRAQVRAGMN |
| Enzyme Length | 358 |
| Uniprot Accession Number | P0CAW2 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 94; /note="Target strand DNA"; /evidence="ECO:0000269|PubMed:31601797, ECO:0007744|PDB:6PBD"; BINDING 164; /note="Non-target strand DNA"; /evidence="ECO:0000269|PubMed:31601797, ECO:0007744|PDB:6PBD"; BINDING 179; /note="Target strand DNA"; /evidence="ECO:0000269|PubMed:31601797, ECO:0007744|PDB:6PBD"; BINDING 267; /note="Non-target strand DNA"; /evidence="ECO:0000269|PubMed:31601797, ECO:0007744|PDB:6PBD"; BINDING 272; /note="Target strand DNA"; /evidence="ECO:0000269|PubMed:31601797, ECO:0007744|PDB:6PBD"; BINDING 350; /note="Non-target strand DNA"; /evidence="ECO:0000269|PubMed:31601797, ECO:0007744|PDB:6PBD" |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72; |
| DNA Binding | DNA_BIND 31..34; /note="Target strand DNA"; /evidence="ECO:0000269|PubMed:31601797, ECO:0007744|PDB:6PBD"; DNA_BIND 39..45; /note="Target strand DNA"; /evidence="ECO:0000269|PubMed:31601797, ECO:0007744|PDB:6PBD"; DNA_BIND 93..94; /note="Non-target strand DNA"; /evidence="ECO:0000269|PubMed:31601797, ECO:0007744|PDB:6PBD"; DNA_BIND 109..110; /note="Non-target strand DNA"; /evidence="ECO:0000269|PubMed:31601797, ECO:0007744|PDB:6PBD"; DNA_BIND 122..132; /note="Target strand DNA"; /evidence="ECO:0000269|PubMed:31601797, ECO:0007744|PDB:6PBD"; DNA_BIND 153..157; /note="Non-target strand DNA"; /evidence="ECO:0000269|PubMed:31601797, ECO:0007744|PDB:6PBD"; DNA_BIND 187..193; /note="Target strand DNA"; /evidence="ECO:0000269|PubMed:31601797, ECO:0007744|PDB:6PBD"; DNA_BIND 315..317; /note="Non-target strand DNA"; /evidence="ECO:0000269|PubMed:31601797, ECO:0007744|PDB:6PBD"; DNA_BIND 330..332; /note="Non-target strand DNA"; /evidence="ECO:0000269|PubMed:31601797, ECO:0007744|PDB:6PBD" |
| EC Number | 2.1.1.72 |
| Enzyme Function | FUNCTION: A beta subtype methylase that recognizes the double-stranded sequence 5'-GANTC-3' and methylates non-modifed A-2 on the hemimethylated, post-replicative DNA (Probable) (PubMed:12654995) (By similarity). Opens a bubble in the DNA at the recognition site, allowing precise recognition of the sequence and ensuring enzyme specificity (PubMed:31601797). Functions only in the predivisional cell. Responsible for 5'-GANTC-3' methylation in the cell; methylation of hemimethylated sites generated after replication fork passage occurs late in the predivisional cell, near completion of chromosome replication but prior to cell division. Contributes to the accurate cell-cycle control of DNA replication and cellular morphology (By similarity). {ECO:0000250|UniProtKB:B8GZ33, ECO:0000269|PubMed:31601797, ECO:0000303|PubMed:12654995, ECO:0000305|PubMed:31601797}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (17); Binding site (6); Chain (1); DNA binding (9); Domain (1); Helix (12); Region (3); Turn (5) |
| Keywords | 3D-structure;DNA replication;DNA-binding;Methyltransferase;Reference proteome;S-adenosyl-L-methionine;Transferase |
| Interact With | |
| Induction | INDUCTION: Transcribed from 80 minutes in synchronized cells. {ECO:0000250|UniProtKB:B8GZ33}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Rapidly degraded by Lon protease prior to cell division. {ECO:0000250|UniProtKB:B8GZ33}. |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 6PBD; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 39,665 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15197 |
| Cross Reference Brenda | 2.1.1.72; |