Detail Information for IndEnz0002016685
IED ID IndEnz0002016685
Enzyme Type ID protease016685
Protein Name Probable cytosol aminopeptidase
EC 3.4.11.1
Leucine aminopeptidase
LAP
EC 3.4.11.10
Leucyl aminopeptidase
Gene Name pepA VP2644
Organism Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio Vibrio harveyi group Vibrio parahaemolyticus Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Enzyme Sequence MEFSVKSGSPEKQRSACIVVGVFEPRRLSPVAEQLDKISDGYISSLLRRGDLEGKPGQMLLLHQVPGVLSERVLLVGCGKERELGERQYKEIIQKTISTLNETGSMEAVCFLTELHVKGRDTYWKVRQAVEATKDGLYTFNQFKSVKPETRRPLRKLVFNVPTRRELSLGEKAITHGLAIASGVKASKDLGNMPPNVANPAYLASQARRLADDYESVTTKIIGEQEMEKLGMTSYLAVGRGSKNESMMSIIEYKGNPDSDAKPIVLVGKGLTFDSGGISLKPGEGMDEMKYDMCGAASVFGTMKALAKLNLPINVIGVLAGCENMPGSNAYRPGDILTTMSGQTVEVLNTDAEGRLVLCDALTYVERFEPDCVVDVATLTGACVIALGHHISGVLSNHNPLAHELVNASEQSSDRAWRLPMADEYHEQLKSPFADMANIGGRPGGTITAGCFLSKFAKKYNWAHIDIAGTAWKSGAAKGSTGRPVSMLVQFLLNRSGQETEE
Enzyme Length 502
Uniprot Accession Number Q87LG8
Absorption
Active Site ACT_SITE 281; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; ACT_SITE 355; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00181};
DNA Binding
EC Number 3.4.11.1; 3.4.11.10
Enzyme Function FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. {ECO:0000255|HAMAP-Rule:MF_00181}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (7)
Keywords Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease;Reference proteome
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00181}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 54,478
Kinetics
Metal Binding METAL 269; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 274; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 274; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 292; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 351; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 353; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 353; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Rhea ID
Cross Reference Brenda