Detail Information for IndEnz0002016710
IED ID IndEnz0002016710
Enzyme Type ID protease016710
Protein Name Chymotrypsin-like elastase family member 1
EC 3.4.21.36
Elastase-1
Gene Name CELA1 ELA1
Organism Sus scrofa (Pig)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Suina Suidae (pigs) Sus Sus scrofa (Pig)
Enzyme Sequence MLRLLVVASLVLYGHSTQDFPETNARVVGGTEAQRNSWPSQISLQYRSGSSWAHTCGGTLIRQNWVMTAAHCVDRELTFRVVVGEHNLNQNDGTEQYVGVQKIVVHPYWNTDDVAAGYDIALLRLAQSVTLNSYVQLGVLPRAGTILANNSPCYITGWGLTRTNGQLAQTLQQAYLPTVDYAICSSSSYWGSTVKNSMVCAGGDGVRSGCQGDSGGPLHCLVNGQYAVHGVTSFVSRLGCNVTRKPTVFTRVSAYISWINNVIASN
Enzyme Length 266
Uniprot Accession Number P00772
Absorption
Active Site ACT_SITE 71; /note="Charge relay system"; /evidence="ECO:0000269|PubMed:4578945, ECO:0000269|PubMed:5415110"; ACT_SITE 119; /note="Charge relay system"; /evidence="ECO:0000269|PubMed:5415110"; ACT_SITE 214; /note="Charge relay system"; /evidence="ECO:0000269|PubMed:5415110"
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.; EC=3.4.21.36;
DNA Binding
EC Number 3.4.21.36
Enzyme Function FUNCTION: Acts upon elastin.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Beta strand (19); Chain (1); Disulfide bond (4); Domain (1); Helix (6); Metal binding (3); Propeptide (1); Sequence conflict (4); Signal peptide (1); Turn (3)
Keywords 3D-structure;Calcium;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen
Interact With P01009
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..16
Structure 3D X-ray crystallography (121)
Cross Reference PDB 1B0E; 1BMA; 1BTU; 1C1M; 1E34; 1E35; 1E36; 1E37; 1E38; 1EAI; 1EAS; 1EAT; 1EAU; 1ELA; 1ELB; 1ELC; 1ELD; 1ELE; 1ELF; 1ELG; 1ESA; 1ESB; 1EST; 1FLE; 1FZZ; 1GVK; 1GWA; 1H9L; 1HAX; 1HAY; 1HAZ; 1HB0; 1HV7; 1INC; 1JIM; 1L0Z; 1L1G; 1LKA; 1LKB; 1LVY; 1MCV; 1MMJ; 1NES; 1OKX; 1QGF; 1QIX; 1QNJ; 1QR3; 1UO6; 1UVO; 1UVP; 2A7C; 2A7J; 2BB4; 2BD2; 2BD3; 2BD4; 2BD5; 2BD7; 2BD8; 2BD9; 2BDA; 2BDB; 2BDC; 2BLO; 2BLQ; 2CV3; 2D26; 2DE8; 2DE9; 2EST; 2FO9; 2FOA; 2FOB; 2FOC; 2FOD; 2FOE; 2FOF; 2FOG; 2FOH; 2G4T; 2G4U; 2H1U; 2IOT; 2OQU; 2V0B; 2V35; 3E3T; 3EST; 3HGN; 3HGP; 3MNB; 3MNC; 3MNS; 3MNX; 3MO3; 3MO6; 3MO9; 3MOC; 3MTY; 3MU0; 3MU1; 3MU4; 3MU5; 3MU8; 3ODD; 3ODF; 3UOU; 4EST; 4GVU; 4YM9; 5AVD; 5EST; 6EST; 6Q8S; 6QBU; 6QEN; 6QEO; 6TH7; 7EST; 8EST; 9EST;
Mapped Pubmed ID 10387042; 10738204; 10739939; 10866385; 11023818; 11294386; 11310599; 11358514; 11473259; 11896054; 12037300; 12198296; 12198297; 12467609; 12554935; 14583266; 14684889; 15299973; 15840915; 16131756; 16131760; 16321984; 16488429; 16511165; 16754679; 16820677; 17083959; 17266656; 17327674; 17452791; 18019537; 19020357; 19603802; 1998677; 20507992; 20944241; 22435400; 23350733; 2337582; 2354062; 25878249; 2611205; 26372354; 27465595; 31935054; 32176480; 3271103; 33002681; 3430613; 3640831; 5415109; 6715339; 6926029; 7604279; 7656008; 7779821; 7837235; 7837246; 7880814; 7932559; 8049229; 8794736; 9187653; 9443341; 9651152; 9860865;
Motif
Gene Encoded By
Mass 28,821
Kinetics
Metal Binding METAL 85; /note=Calcium; METAL 90; /note=Calcium; via carbonyl oxygen; METAL 95; /note=Calcium
Rhea ID
Cross Reference Brenda 3.4.21.36;