| IED ID | IndEnz0002016711 |
| Enzyme Type ID | protease016711 |
| Protein Name |
Chymotrypsin-like elastase family member 1 EC 3.4.21.36 Elastase-1 |
| Gene Name | Cela1 Ela1 |
| Organism | Rattus norvegicus (Rat) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat) |
| Enzyme Sequence | MLRFLVFASLVLYGHSTQDFPETNARVVGGAEARRNSWPSQISLQYLSGGSWYHTCGGTLIRRNWVMTAAHCVSSQMTFRVVVGDHNLSQNDGTEQYVSVQKIMVHPTWNSNNVAAGYDIALLRLAQSVTLNNYVQLAVLPQEGTILANNNPCYITGWGRTRTNGQLSQTLQQAYLPSVDYSICSSSSYWGSTVKTTMVCAGGDGVRSGCQGDSGGPLHCLVNGQYSVHGVTSFVSSMGCNVSKKPTVFTRVSAYISWMNNVIAYT |
| Enzyme Length | 266 |
| Uniprot Accession Number | P00773 |
| Absorption | |
| Active Site | ACT_SITE 71; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 119; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 214; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.; EC=3.4.21.36; |
| DNA Binding | |
| EC Number | 3.4.21.36 |
| Enzyme Function | FUNCTION: Acts upon elastin. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (4); Domain (1); Metal binding (3); Propeptide (1); Sequence conflict (4); Signal peptide (1) |
| Keywords | Calcium;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000269|PubMed:6555050 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14631510; 14631512; |
| Motif | |
| Gene Encoded By | |
| Mass | 28,976 |
| Kinetics | |
| Metal Binding | METAL 85; /note=Calcium; /evidence=ECO:0000250; METAL 90; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250; METAL 95; /note=Calcium; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |