Detail Information for IndEnz0002016720
IED ID IndEnz0002016720
Enzyme Type ID protease016720
Protein Name D-alanyl-D-alanine-carboxypeptidase/endopeptidase AmpH
EC 3.4.-.-
DD-alanine-endopeptidase
DD-carboxypeptidase
Penicillin-binding protein AmpH
Gene Name ampH yaiH b0376 JW5052
Organism Escherichia coli (strain K12)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12)
Enzyme Sequence MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFYGSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLDQGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPTREQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMKDTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQRSNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGFITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS
Enzyme Length 385
Uniprot Accession Number P0AD70
Absorption
Active Site
Activity Regulation ACTIVITY REGULATION: Inhibited by cefmetazole. {ECO:0000269|PubMed:22001512}.
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.-.-
Enzyme Function FUNCTION: Hydrolyzes the cross-linked dimers tetrapentapeptide (D45) and tetratetrapeptide (D44). Removes the terminal D-alanine from muropeptides and disaccharide pentapeptide M5 with a C-terminal D-Ala-D-Ala dipeptide. Associated with recycling and remodeling of peptidoglycan (PG). Also displays a low beta-lactamase activity. {ECO:0000269|PubMed:22001512}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Erroneous initiation (1); Signal peptide (1)
Keywords Carboxypeptidase;Cell inner membrane;Cell membrane;Cell shape;Cell wall biogenesis/degradation;Direct protein sequencing;Hydrolase;Membrane;Protease;Reference proteome;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:22001512}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..21; /evidence=ECO:0000269|PubMed:22001512
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 24561554;
Motif
Gene Encoded By
Mass 41,849
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=102 uM for D45 (at 37 degrees Celsius and pH 7.3) {ECO:0000269|PubMed:22001512}; KM=134 uM for D44 (at 37 degrees Celsius and pH 7.3) {ECO:0000269|PubMed:22001512}; KM=225 uM for M5 (at 37 degrees Celsius and pH 7.5) {ECO:0000269|PubMed:22001512}; Vmax=4.98 nmol/min/ug enzyme with M5 as substrate (at 37 degrees Celsius and pH 7.5) {ECO:0000269|PubMed:22001512}; Vmax=162 nmol/min/ug enzyme with D44 as substrate (at 37 degrees Celsius and pH 7.3) {ECO:0000269|PubMed:22001512}; Vmax=174 nmol/min/ug enzyme with D45 as substrate (at 37 degrees Celsius and pH 7.3) {ECO:0000269|PubMed:22001512};
Metal Binding
Rhea ID
Cross Reference Brenda