IED ID | IndEnz0002016722 |
Enzyme Type ID | protease016722 |
Protein Name |
Lysyl aminopeptidase EC 3.4.11.15 |
Gene Name | PF1861 |
Organism | Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) |
Taxonomic Lineage | cellular organisms Archaea Euryarchaeota Thermococci Thermococcales Thermococcaceae Pyrococcus Pyrococcus furiosus Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) |
Enzyme Sequence | MVDWELMKKIIESPGVSGYEHLGIRDLVVDILKDVADEVKIDKLGNVIAHFKGSAPKVMVAAHMDKIGLMVNHIDKDGYLRVVPIGGVLPETLIAQKIRFFTEKGERYGVVGVLPPHLRREAKDQGGKIDWDSIIVDVGASSREEAEEMGFRIGTIGEFAPNFTRLSEHRFATPYLDDRICLYAMIEAARQLGEHEADIYIVASVQEEIGLRGARVASFAIDPEVGIAMDVTFAKQPNDKGKIVPELGKGPVMDVGPNINPKLRQFADEVAKKYEIPLQVEPSPRPTGTDANVMQINREGVATAVLSIPIRYMHSQVELADARDVDNTIKLAKALLEELKPMDFTP |
Enzyme Length | 346 |
Uniprot Accession Number | Q8TZW4 |
Absorption | |
Active Site | ACT_SITE 207; /note=Proton acceptor; /evidence=ECO:0000250 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferentially, release of N-terminal lysine.; EC=3.4.11.15; Evidence={ECO:0000269|PubMed:15743956}; |
DNA Binding | |
EC Number | 3.4.11.15 |
Enzyme Function | FUNCTION: Hydrolyzes di-, tri- and tetrapeptides with a lysine as the N-terminal amino acid and with Gly, Lys, Ala, Phe or Glu in the second position. |
Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 100 degrees Celsius. {ECO:0000269|PubMed:15743956}; |
PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8. {ECO:0000269|PubMed:15743956}; |
Pathway | |
nucleotide Binding | |
Features | Active site (1); Beta strand (17); Chain (1); Helix (11); Metal binding (6); Turn (3) |
Keywords | 3D-structure;Aminopeptidase;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Zinc |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | X-ray crystallography (1) |
Cross Reference PDB | 4X8I; |
Mapped Pubmed ID | 26853450; |
Motif | |
Gene Encoded By | |
Mass | 38,214 |
Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=4.6 mM for Lys-pNA {ECO:0000269|PubMed:15743956}; KM=1.8 mM for Lys-Gly-Gly {ECO:0000269|PubMed:15743956}; KM=1.4 mM for Arg-pNA {ECO:0000269|PubMed:15743956}; Vmax=2900 umol/min/mg enzyme with Lys-pNA as substrate {ECO:0000269|PubMed:15743956}; Vmax=2300 umol/min/mg enzyme with Lys-Gly-Gly as substrate {ECO:0000269|PubMed:15743956}; Vmax=1200 umol/min/mg enzyme with Arg-pNA as substrate {ECO:0000269|PubMed:15743956}; |
Metal Binding | METAL 63; /note=Zinc 1; /evidence=ECO:0000250; METAL 177; /note=Zinc 1; /evidence=ECO:0000250; METAL 177; /note=Zinc 2; /evidence=ECO:0000250; METAL 208; /note=Zinc 2; /evidence=ECO:0000250; METAL 230; /note=Zinc 1; /evidence=ECO:0000250; METAL 314; /note=Zinc 2; /evidence=ECO:0000250 |
Rhea ID | |
Cross Reference Brenda | 3.4.11.15; |