| IED ID | IndEnz0002016726 |
| Enzyme Type ID | protease016726 |
| Protein Name |
Putative leucine aminopeptidase 1 EC 3.4.11.1 Leucyl aminopeptidase 1 LAP 1 Proline aminopeptidase 1 EC 3.4.11.5 Prolyl aminopeptidase 1 |
| Gene Name | Os12g0434400 LOC_Os12g24650 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MPNVVDPPQISFAAKDMDLTEWEGDILAVLVTETDVSKATSSSSRFTNAAAALAKLDGELGGLLSEASAEEEFAGRAGQSVALRLPTAPGLHGFKRVCLVGVGNNMPSSAAACRSTGETIAAVAKSAQARSAAVALASPPPGWVQGEDLRLNAAAAVASGVVLGLHEDRRYKSDSKKVHLKQVDLIGFGSGQEMGRKLQYANHVSSAVIFAKELVNSPANVLTPAVLAEEASNIASSYSDVLTATILDEEKCRELKMGSYLAVAAASANPPHFIHLCYKPPGGNVKRKLAIVGKGLTFDRFYLSLDNLLIVTKFVCIGGYNIKIGAVTTIELMKKDMGGSAALFGAAKALGQIKPPGVEVHFISAACENMISGTGMRPGDIVTASNGKTIEVDNTDAEGRLTLADALVYACKLGVDKIIDLATLTGYCRIALGPSIAGILTPSDELDKEVAAAYEASGEKFWRLPLEESYWEQMKSSVADMLNTGSPLGGAITAGLFLKQFVDEKVKWMHVDMAGPVWNYKKQEATGFGVSTLVEWVLINSS |
| Enzyme Length | 542 |
| Uniprot Accession Number | Q2QSB9 |
| Absorption | |
| Active Site | ACT_SITE 323; /evidence=ECO:0000255; ACT_SITE 400; /evidence=ECO:0000255 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; CATALYTIC ACTIVITY: Reaction=Release of N-terminal proline from a peptide.; EC=3.4.11.5; |
| DNA Binding | |
| EC Number | 3.4.11.1; 3.4.11.5 |
| Enzyme Function | FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Erroneous gene model prediction (1); Metal binding (7) |
| Keywords | Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 57,142 |
| Kinetics | |
| Metal Binding | METAL 294; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 299; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 299; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184; METAL 336; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 396; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184; METAL 398; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 398; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184 |
| Rhea ID | |
| Cross Reference Brenda |