Detail Information for IndEnz0002016726
IED ID IndEnz0002016726
Enzyme Type ID protease016726
Protein Name Putative leucine aminopeptidase 1
EC 3.4.11.1
Leucyl aminopeptidase 1
LAP 1
Proline aminopeptidase 1
EC 3.4.11.5
Prolyl aminopeptidase 1
Gene Name Os12g0434400 LOC_Os12g24650
Organism Oryza sativa subsp. japonica (Rice)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice)
Enzyme Sequence MPNVVDPPQISFAAKDMDLTEWEGDILAVLVTETDVSKATSSSSRFTNAAAALAKLDGELGGLLSEASAEEEFAGRAGQSVALRLPTAPGLHGFKRVCLVGVGNNMPSSAAACRSTGETIAAVAKSAQARSAAVALASPPPGWVQGEDLRLNAAAAVASGVVLGLHEDRRYKSDSKKVHLKQVDLIGFGSGQEMGRKLQYANHVSSAVIFAKELVNSPANVLTPAVLAEEASNIASSYSDVLTATILDEEKCRELKMGSYLAVAAASANPPHFIHLCYKPPGGNVKRKLAIVGKGLTFDRFYLSLDNLLIVTKFVCIGGYNIKIGAVTTIELMKKDMGGSAALFGAAKALGQIKPPGVEVHFISAACENMISGTGMRPGDIVTASNGKTIEVDNTDAEGRLTLADALVYACKLGVDKIIDLATLTGYCRIALGPSIAGILTPSDELDKEVAAAYEASGEKFWRLPLEESYWEQMKSSVADMLNTGSPLGGAITAGLFLKQFVDEKVKWMHVDMAGPVWNYKKQEATGFGVSTLVEWVLINSS
Enzyme Length 542
Uniprot Accession Number Q2QSB9
Absorption
Active Site ACT_SITE 323; /evidence=ECO:0000255; ACT_SITE 400; /evidence=ECO:0000255
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; CATALYTIC ACTIVITY: Reaction=Release of N-terminal proline from a peptide.; EC=3.4.11.5;
DNA Binding
EC Number 3.4.11.1; 3.4.11.5
Enzyme Function FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Erroneous gene model prediction (1); Metal binding (7)
Keywords Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease;Reference proteome
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 57,142
Kinetics
Metal Binding METAL 294; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 299; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 299; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184; METAL 336; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 396; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184; METAL 398; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 398; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184
Rhea ID
Cross Reference Brenda