Detail Information for IndEnz0002016727
IED ID IndEnz0002016727
Enzyme Type ID protease016727
Protein Name Leucine aminopeptidase 1, chloroplastic
EC 3.4.11.1
DR57
Leucyl aminopeptidase 1
LAP 1
Proline aminopeptidase 1
EC 3.4.11.5
Prolyl aminopeptidase 1
Gene Name LAPA1 LAP LAP2 Solyc12g010020
Organism Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Solanoideae Solaneae Solanum Solanum subgen. Lycopersicon Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Enzyme Sequence MATLRVSSLFASSSSSLHSNPSVFTKYQSSPKWAFSFPVTPLCSKRSKRIVHCIAGDTLGLTRPNESDAPKISIGAKDTAVVQWQGDLLAIGATENDMARDENSKFKNPLLQQLDSELNGLLSAASSEEDFSGKSGQSVNLRFPGGRITLVGLGSSASSPTSYHSLGQAAAAAAKSSQARNIAVALASTDGLSAESKINSASAIATGVVLGSFEDNRFRSESKKSTLESLDILGLGTGPEIERKIKYAEHVCAGVILGRELVNAPANIVTPAVLAEEAKKIASTYSDVISVNILDAEQCKELKMGAYLAVAAAATENPPYFIHLCFKTPTKERKTKLALVGKGLTFDSGGYNLKVGAGSRIELMKNDMGGAAAVLGAAKALGEIRPSRVEVHFIVAACENMISAEGMRPGDIVTASNGKTIEVNNTDAEGRLTLADALIYACNQGVEKIIDLATLTGAIMVALGPSVAGAFTPNDDLAREVVEAAEASGEKLWRMPMEESYWESMKSGSGDMINTGPGNGGAITGALFLKQFVDEKVQWLHLDVAGPVWSDEKKNATGYGVSTLVEWVLRN
Enzyme Length 571
Uniprot Accession Number Q10712
Absorption
Active Site ACT_SITE 354; /evidence=ECO:0000255; ACT_SITE 431; /evidence=ECO:0000255
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; CATALYTIC ACTIVITY: Reaction=Release of N-terminal proline from a peptide.; EC=3.4.11.5;
DNA Binding
EC Number 3.4.11.1; 3.4.11.5
Enzyme Function FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Beta strand (17); Chain (1); Helix (19); Metal binding (7); Sequence conflict (7); Transit peptide (1); Turn (5)
Keywords 3D-structure;Aminopeptidase;Chloroplast;Hydrolase;Magnesium;Metal-binding;Plastid;Protease;Reference proteome;Transit peptide
Interact With
Induction INDUCTION: Induced by wounding (PubMed:8234334, PubMed:15231736). Induced by methyl jasmonate (PubMed:15231736). {ECO:0000269|PubMed:15231736, ECO:0000269|PubMed:8234334}.
Subcellular Location SUBCELLULAR LOCATION: Plastid, chloroplast.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (2)
Cross Reference PDB 4KSI; 5D8N;
Mapped Pubmed ID 27139632;
Motif
Gene Encoded By
Mass 60,154
Kinetics
Metal Binding METAL 342; /note="Magnesium 1"; /evidence="ECO:0000269|PubMed:24914976, ECO:0007744|PDB:4KSI"; METAL 347; /note="Magnesium 1"; /evidence="ECO:0000269|PubMed:24914976, ECO:0007744|PDB:4KSI"; METAL 347; /note="Magnesium 2"; /evidence="ECO:0000269|PubMed:24914976, ECO:0007744|PDB:4KSI"; METAL 367; /note="Magnesium 1"; /evidence="ECO:0000269|PubMed:24914976, ECO:0007744|PDB:4KSI"; METAL 427; /note="Magnesium 2"; /evidence="ECO:0000269|PubMed:24914976, ECO:0007744|PDB:4KSI"; METAL 429; /note="Magnesium 1"; /evidence="ECO:0000269|PubMed:24914976, ECO:0007744|PDB:4KSI"; METAL 429; /note="Magnesium 2"; /evidence="ECO:0000269|PubMed:24914976, ECO:0007744|PDB:4KSI"
Rhea ID
Cross Reference Brenda 3.4.11.1;