Detail Information for IndEnz0002016735
IED ID IndEnz0002016735
Enzyme Type ID protease016735
Protein Name Leucine aminopeptidase 2, chloroplastic
EC 3.4.11.1
Leucyl aminopeptidase 2
LAP 2
Proline aminopeptidase 2
EC 3.4.11.5
Prolyl aminopeptidase 2
Gene Name Os02g0794700 LOC_Os02g55140 OJ1695_H09.4 P0700F06.36
Organism Oryza sativa subsp. japonica (Rice)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice)
Enzyme Sequence MATAASTSAAAVAAASRLLVRRAPPRLLRRLPRAALAASRPSPPSSSSYGAAAVALGRQPLGHRARMGHTAAAAAAAGPALGLTKPNAVEPPQVSFAAKDVEFSEWKGDILAIAVTENDLVKGSDSKFENAVLKKLDGQLGGLLSEASAEEDFTGKAGQSVVLRLPGQGFKRVGLIGLGQNAPSTTTACKGIGESVASVAKSAQASSAAIVFASVGGIQEDFKLTAAAAIASGTVLGLHEDSRYKSESKKVHLKQVDLIGFGSGPEVDQKLKYANDLSSGVIFGKELVNSPANVLTPAVLAEEASNIASTYSDVFTATILDVEKCKELKMGSYLGVAAASANPPHFIHLCYKPPGGNAKRKLAIVGKGLTFDSGGYNIKTGPGCSIELMKFDMGGSAAVFGAAKALGQIKPPGVEVHFIVAACENMISGTGMRPGDIVTASNGKTIEVNNTDAEGRLTLADALVYACNQGVDKIIDLATLTGACVVALGPSIAGIFTPSDELAKEVAAASEISGEKFWRMPLEESYWESMKSGVADMVNTGGRQGGSITAALFLKQFVDEKVQWMHIDMAGPVWNDKKRAATGFGVSTLVEWVLKNSS
Enzyme Length 598
Uniprot Accession Number Q6K669
Absorption
Active Site ACT_SITE 379; /evidence=ECO:0000255; ACT_SITE 456; /evidence=ECO:0000255
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; CATALYTIC ACTIVITY: Reaction=Release of N-terminal proline from a peptide.; EC=3.4.11.5;
DNA Binding
EC Number 3.4.11.1; 3.4.11.5
Enzyme Function FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Erroneous gene model prediction (3); Metal binding (7); Transit peptide (1)
Keywords Aminopeptidase;Chloroplast;Hydrolase;Manganese;Metal-binding;Plastid;Protease;Reference proteome;Transit peptide
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 61,819
Kinetics
Metal Binding METAL 367; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 372; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 372; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184; METAL 392; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 452; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184; METAL 454; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 454; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184
Rhea ID
Cross Reference Brenda