| IED ID | IndEnz0002016735 |
| Enzyme Type ID | protease016735 |
| Protein Name |
Leucine aminopeptidase 2, chloroplastic EC 3.4.11.1 Leucyl aminopeptidase 2 LAP 2 Proline aminopeptidase 2 EC 3.4.11.5 Prolyl aminopeptidase 2 |
| Gene Name | Os02g0794700 LOC_Os02g55140 OJ1695_H09.4 P0700F06.36 |
| Organism | Oryza sativa subsp. japonica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. japonica (Rice) |
| Enzyme Sequence | MATAASTSAAAVAAASRLLVRRAPPRLLRRLPRAALAASRPSPPSSSSYGAAAVALGRQPLGHRARMGHTAAAAAAAGPALGLTKPNAVEPPQVSFAAKDVEFSEWKGDILAIAVTENDLVKGSDSKFENAVLKKLDGQLGGLLSEASAEEDFTGKAGQSVVLRLPGQGFKRVGLIGLGQNAPSTTTACKGIGESVASVAKSAQASSAAIVFASVGGIQEDFKLTAAAAIASGTVLGLHEDSRYKSESKKVHLKQVDLIGFGSGPEVDQKLKYANDLSSGVIFGKELVNSPANVLTPAVLAEEASNIASTYSDVFTATILDVEKCKELKMGSYLGVAAASANPPHFIHLCYKPPGGNAKRKLAIVGKGLTFDSGGYNIKTGPGCSIELMKFDMGGSAAVFGAAKALGQIKPPGVEVHFIVAACENMISGTGMRPGDIVTASNGKTIEVNNTDAEGRLTLADALVYACNQGVDKIIDLATLTGACVVALGPSIAGIFTPSDELAKEVAAASEISGEKFWRMPLEESYWESMKSGVADMVNTGGRQGGSITAALFLKQFVDEKVQWMHIDMAGPVWNDKKRAATGFGVSTLVEWVLKNSS |
| Enzyme Length | 598 |
| Uniprot Accession Number | Q6K669 |
| Absorption | |
| Active Site | ACT_SITE 379; /evidence=ECO:0000255; ACT_SITE 456; /evidence=ECO:0000255 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; CATALYTIC ACTIVITY: Reaction=Release of N-terminal proline from a peptide.; EC=3.4.11.5; |
| DNA Binding | |
| EC Number | 3.4.11.1; 3.4.11.5 |
| Enzyme Function | FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Erroneous gene model prediction (3); Metal binding (7); Transit peptide (1) |
| Keywords | Aminopeptidase;Chloroplast;Hydrolase;Manganese;Metal-binding;Plastid;Protease;Reference proteome;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 61,819 |
| Kinetics | |
| Metal Binding | METAL 367; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 372; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 372; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184; METAL 392; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 452; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184; METAL 454; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 454; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184 |
| Rhea ID | |
| Cross Reference Brenda |