IED ID | IndEnz0002016737 |
Enzyme Type ID | protease016737 |
Protein Name |
Archaemetzincin EC 3.4.-.- |
Gene Name | amzA MK0548 |
Organism | Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) |
Taxonomic Lineage | cellular organisms Archaea Euryarchaeota Methanopyri Methanopyrales Methanopyraceae Methanopyrus Methanopyrus kandleri Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) |
Enzyme Sequence | MKLCLVAFDGRIPMLSSIVDRFEEHVSEYLGEVKVKKKRAKLPEHAYSKVRGQYLARALLDTLRGMKGEYDRVLGLTSEDLYAPGLNFVFGQARCPGREAVVSVARLLDPDPELYLERVVKELTHELGHTFGLGHCPDRNCVMSFSSSLLEVDRKSPNFCRRCTELLQRNLKRGG |
Enzyme Length | 175 |
Uniprot Accession Number | Q8TXW1 |
Absorption | |
Active Site | ACT_SITE 126; /note=Proton acceptor; /evidence=ECO:0000305 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | |
DNA Binding | |
EC Number | 3.4.-.- |
Enzyme Function | FUNCTION: Probable zinc metalloprotease whose natural substrate is unknown. Does not show endo- or exopeptidase activity against resorufin labeled casein, p-nitroanilide (pNA), amidomethylcoumarin (AMC) (one to three amino acids in length), and hippuryl-aminoacid substrates. {ECO:0000255|HAMAP-Rule:MF_01842, ECO:0000269|PubMed:20597090}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (1); Beta strand (6); Chain (1); Helix (6); Metal binding (7); Mutagenesis (1); Turn (2) |
Keywords | 3D-structure;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Zinc |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | X-ray crystallography (1) |
Cross Reference PDB | 2X7M; |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 19,827 |
Kinetics | |
Metal Binding | METAL 125; /note="Zinc 1; via pros nitrogen; catalytic"; /evidence="ECO:0000255|HAMAP-Rule:MF_01842, ECO:0000269|PubMed:20597090"; METAL 129; /note="Zinc 1; via pros nitrogen; catalytic"; /evidence="ECO:0000255|HAMAP-Rule:MF_01842, ECO:0000269|PubMed:20597090"; METAL 135; /note="Zinc 1; via pros nitrogen; catalytic"; /evidence="ECO:0000255|HAMAP-Rule:MF_01842, ECO:0000269|PubMed:20597090"; METAL 136; /note="Zinc 2"; /evidence="ECO:0000255|HAMAP-Rule:MF_01842, ECO:0000269|PubMed:20597090"; METAL 141; /note="Zinc 2"; /evidence="ECO:0000255|HAMAP-Rule:MF_01842, ECO:0000269|PubMed:20597090"; METAL 160; /note="Zinc 2"; /evidence="ECO:0000255|HAMAP-Rule:MF_01842, ECO:0000269|PubMed:20597090"; METAL 163; /note="Zinc 2"; /evidence="ECO:0000255|HAMAP-Rule:MF_01842, ECO:0000269|PubMed:20597090" |
Rhea ID | |
Cross Reference Brenda |