| IED ID | IndEnz0002016737 |
| Enzyme Type ID | protease016737 |
| Protein Name |
Archaemetzincin EC 3.4.-.- |
| Gene Name | amzA MK0548 |
| Organism | Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) |
| Taxonomic Lineage | cellular organisms Archaea Euryarchaeota Methanopyri Methanopyrales Methanopyraceae Methanopyrus Methanopyrus kandleri Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) |
| Enzyme Sequence | MKLCLVAFDGRIPMLSSIVDRFEEHVSEYLGEVKVKKKRAKLPEHAYSKVRGQYLARALLDTLRGMKGEYDRVLGLTSEDLYAPGLNFVFGQARCPGREAVVSVARLLDPDPELYLERVVKELTHELGHTFGLGHCPDRNCVMSFSSSLLEVDRKSPNFCRRCTELLQRNLKRGG |
| Enzyme Length | 175 |
| Uniprot Accession Number | Q8TXW1 |
| Absorption | |
| Active Site | ACT_SITE 126; /note=Proton acceptor; /evidence=ECO:0000305 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.-.- |
| Enzyme Function | FUNCTION: Probable zinc metalloprotease whose natural substrate is unknown. Does not show endo- or exopeptidase activity against resorufin labeled casein, p-nitroanilide (pNA), amidomethylcoumarin (AMC) (one to three amino acids in length), and hippuryl-aminoacid substrates. {ECO:0000255|HAMAP-Rule:MF_01842, ECO:0000269|PubMed:20597090}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (6); Chain (1); Helix (6); Metal binding (7); Mutagenesis (1); Turn (2) |
| Keywords | 3D-structure;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 2X7M; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 19,827 |
| Kinetics | |
| Metal Binding | METAL 125; /note="Zinc 1; via pros nitrogen; catalytic"; /evidence="ECO:0000255|HAMAP-Rule:MF_01842, ECO:0000269|PubMed:20597090"; METAL 129; /note="Zinc 1; via pros nitrogen; catalytic"; /evidence="ECO:0000255|HAMAP-Rule:MF_01842, ECO:0000269|PubMed:20597090"; METAL 135; /note="Zinc 1; via pros nitrogen; catalytic"; /evidence="ECO:0000255|HAMAP-Rule:MF_01842, ECO:0000269|PubMed:20597090"; METAL 136; /note="Zinc 2"; /evidence="ECO:0000255|HAMAP-Rule:MF_01842, ECO:0000269|PubMed:20597090"; METAL 141; /note="Zinc 2"; /evidence="ECO:0000255|HAMAP-Rule:MF_01842, ECO:0000269|PubMed:20597090"; METAL 160; /note="Zinc 2"; /evidence="ECO:0000255|HAMAP-Rule:MF_01842, ECO:0000269|PubMed:20597090"; METAL 163; /note="Zinc 2"; /evidence="ECO:0000255|HAMAP-Rule:MF_01842, ECO:0000269|PubMed:20597090" |
| Rhea ID | |
| Cross Reference Brenda |