Detail Information for IndEnz0002016740
IED ID IndEnz0002016740
Enzyme Type ID protease016740
Protein Name Leucine aminopeptidase 3, chloroplastic
EC 3.4.11.1
Leucyl aminopeptidase 3
AtLAP3
Proline aminopeptidase 3
EC 3.4.11.5
Prolyl aminopeptidase 3
Gene Name LAP3 At4g30910 F6I18.180
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MAVTLVTSCASSSRFHFRSFSSSPSSLSSCFVRFQLLSRLRVSFAITPLYCSSKATAHTIAHATLGLTQANSVDHPKISFSGKEIDVTEWKGDILAVGVTEKDMAKDANSKFENPILKKLDAHLGGLLADVSAEEDFSGKPGQSTVLRLPGLGSKRVGLIGLGKSASTPSAFQSLGEAVAAAAKASQASSVAVVLASSESFSDESKLSSASDIASGTVLGLFEDSRYKSESKKPSLKSVVFIGFGTGPELENKLKYAEHVSYGVIFTKELVNSPANVLSPAVLAEEASNLASMYSNVMTANILKEEQCKELKMGSYLAVAAASANPPHFIHLIYKPSSGPVKTKLALVGKGLTFDSGGYNIKIGPELIIELMKIDVGGSAAVLGAAKAIGEIKPPGVEVHFIVAACENMISGTGMRPGDVITASNGKTIEVNDTDSEGRLTLADALVYACNQGVDKIVDIATLTGEIIVALGPSMAGMYTASDELAKEVIAASQRSGEKLWRMPMEESYWEMMKSGVADMVNFGGRAGGSITAALFLKRFVSENVEWLHIDMAGRVWNEKKKAATGFGVATLVEWVQNNSS
Enzyme Length 581
Uniprot Accession Number Q8RX72
Absorption
Active Site ACT_SITE 362; /evidence=ECO:0000255; ACT_SITE 439; /evidence=ECO:0000255
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; CATALYTIC ACTIVITY: Reaction=Release of N-terminal proline from a peptide.; EC=3.4.11.5;
DNA Binding
EC Number 3.4.11.1; 3.4.11.5
Enzyme Function FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity). Possesses Cys-Gly dipeptidase activity (PubMed:25716890). {ECO:0000250|UniProtKB:P30184, ECO:0000269|PubMed:25716890}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Erroneous gene model prediction (2); Metal binding (7); Transit peptide (1)
Keywords Aminopeptidase;Chloroplast;Hydrolase;Manganese;Metal-binding;Plastid;Protease;Reference proteome;Transit peptide
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 12185496; 14576160; 15028209; 27820795; 28627464;
Motif
Gene Encoded By
Mass 61,290
Kinetics
Metal Binding METAL 350; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 355; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 355; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184; METAL 375; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 435; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184; METAL 437; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 437; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184
Rhea ID
Cross Reference Brenda