Detail Information for IndEnz0002016746
IED ID IndEnz0002016746
Enzyme Type ID protease016746
Protein Name Cytosol aminopeptidase
EC 3.4.11.1
Leucine aminopeptidase
LAP
Leucyl aminopeptidase
Proline aminopeptidase
EC 3.4.11.5
Prolyl aminopeptidase
Gene Name lap DDB_G0279793
Organism Dictyostelium discoideum (Slime mold)
Taxonomic Lineage cellular organisms Eukaryota Amoebozoa Evosea Eumycetozoa Dictyostelia (dictyostelid cellular slime molds) Dictyosteliales Dictyosteliaceae Dictyostelium Dictyostelium discoideum (Slime mold)
Enzyme Sequence MLKNIINKKNLFINNQQNIFQIIKRNKMTDSTVDNKGYIVGIYENEEFTPLGQQLNEKTNGHLLKSIKLSDTKGKVGDNLVLYNVTPEVSRVAIVGLGKKENNNSTTYEKNENTRKAIGSGVKALKSKNATHLTIDSNIGDAKQTAEGAFLSNFKFDFKTGTSGKTANSTNESIQVQLSPSPSSEECFKEGKILAESQNFARVLMETPANLLTPTNFVQHVSSQMKELIDSGKVEMIVREEQWVKDQKMGMFWGVAKGSDEPLKFLELHYRGASADGKDSIVYVGKGITFDSGGISIKPSANMGLMKGDMGGAATAVSAMFGVASLGLKVNLITITPLCENMPSGKATKPGDILTAANGKTVEVDNTDAEGRLILGDALHYACSFKPTHIIDIATLTGAIDVALGQHYAGCFTTTDSLWDQLNECGNISGERLWRMPLIPEYRKQMETSKVADLINSAGRSGGACCAAGFLKEFITADQSWSHLDIAGVMSSSEDGPYIRKGMTGKPTRTLIEFAKKNQQ
Enzyme Length 520
Uniprot Accession Number Q5V9F0
Absorption
Active Site ACT_SITE 298; /evidence=ECO:0000250; ACT_SITE 372; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; CATALYTIC ACTIVITY: Reaction=Release of N-terminal proline from a peptide.; EC=3.4.11.5;
DNA Binding
EC Number 3.4.11.1; 3.4.11.5
Enzyme Function FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (7)
Keywords Aminopeptidase;Cytoplasm;Direct protein sequencing;Hydrolase;Metal-binding;Protease;Reference proteome;Zinc
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 1033094; 22120990;
Motif
Gene Encoded By
Mass 56,410
Kinetics
Metal Binding METAL 286; /note=Zinc 2; /evidence=ECO:0000250; METAL 291; /note=Zinc 1; /evidence=ECO:0000250; METAL 291; /note=Zinc 2; /evidence=ECO:0000250; METAL 309; /note=Zinc 2; /evidence=ECO:0000250; METAL 368; /note=Zinc 1; /evidence=ECO:0000250; METAL 370; /note=Zinc 1; /evidence=ECO:0000250; METAL 370; /note=Zinc 2; /evidence=ECO:0000250
Rhea ID
Cross Reference Brenda