Detail Information for IndEnz0002016755
IED ID IndEnz0002016755
Enzyme Type ID protease016755
Protein Name Leucine aminopeptidase, chloroplastic
EC 3.4.11.1
Leucyl aminopeptidase
LAP
Proline aminopeptidase
EC 3.4.11.5
Prolyl aminopeptidase
Gene Name LAP
Organism Solanum tuberosum (Potato)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Solanoideae Solaneae Solanum Solanum tuberosum (Potato)
Enzyme Sequence MATLRVSSLLASSPSSLHCNPSVFTKCQSSPRWAFSFSVTPLCSRRSKRIVHCIAGDTLGLTRPNESDAPKISIGAKDTDVVQWQGDLLAIGATENDLARDDNSKFKNPLLQRLDSKLNGLLSAASSEEDFSGKSGQSINLRLPGGRITLVGLGSSASSPTSYHSLGEAAAAAAKSAQARNIAVSLASTDGLSAESKINSASAIATGVMLGIFEDNRFRSESKTPALESLDILGLGTGPEIESKIKYAEHVCAGVILGRELVNAPANIVTPGALAEEAKKIASTYSDVITVNILDAEQCKELKMGAYLGVAAAATENPPYFIHLCFKTNSRERKTKIALVGKGLTFDSGGYNLKTGAGSKIELMKNDMGGAAAVLGAAKALGEIKPRGVEVHFIVAACENMISGAGMRPGDIVTASNGKTIEVNNTDAEGRLTLADALIYACNQGVEKIIDLATLTGAIVTALGPSVAGAFTPSDGLAREVVVAAEASGEKLWRMPMEESYWESMKSGVADMINTGPRDGGAITGALFLKQFVDEKVQWLHLDIAGPVWSDEKKNATGYGVSTLVEWVLRNSL
Enzyme Length 573
Uniprot Accession Number P31427
Absorption
Active Site ACT_SITE 354; /evidence=ECO:0000255; ACT_SITE 431; /evidence=ECO:0000255
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; CATALYTIC ACTIVITY: Reaction=Release of N-terminal proline from a peptide.; EC=3.4.11.5;
DNA Binding
EC Number 3.4.11.1; 3.4.11.5
Enzyme Function FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (7); Transit peptide (1)
Keywords Aminopeptidase;Chloroplast;Hydrolase;Manganese;Metal-binding;Plastid;Protease;Reference proteome;Stress response;Transit peptide
Interact With
Induction INDUCTION: By abscisic acid (ABA), jasmonic acid (JA) and wounding.
Subcellular Location SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 60,122
Kinetics
Metal Binding METAL 342; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 347; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 347; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184; METAL 367; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 427; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184; METAL 429; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:P30184; METAL 429; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:P30184
Rhea ID
Cross Reference Brenda