IED ID | IndEnz0002016791 |
Enzyme Type ID | protease016791 |
Protein Name |
Microbial collagenase EC 3.4.24.3 |
Gene Name | VV2_1146 |
Organism | Vibrio vulnificus (strain CMCP6) |
Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio Vibrio vulnificus Vibrio vulnificus (strain CMCP6) |
Enzyme Sequence | MSHLLPFPRRRLALACLLASISGASFGQTQCDVAQLQQSPDLATAISSADYACYSGWFSASSDTLNNIYSEASLSRVQVALHQAVQTYQGEAEQARAIENLGEYVRAAYYVRYNAGNVAAFSDLLGQQFAHTINAFLANPHALDQGREQVGAMKSLTLMVDNIKQLPLTMDAMMLALQQFNPETAKNTQWVDGLNNLFRSMAGHIANDAFYRYLASNTQHIDMLEKFANDNAWALDTDADFLVFNALRETGRLIASPDKATKQKAVQVMQRVMARYPLGSEHDKLWLAAVEMLSYFAPEALNGLDLPQAKRDLAARVLPNRHECQGPAIIRSQDLTPEQAAKACDVLAAKEADFHQVANTGMQPVADDHNQRVEVAVFANNDSYVDYSAFLFGNTTDNGGQYLEGNPADEHNTARFVAYRYANGEELSILNLEHEYTHYLDARFNQYGSFSDNLAHGYVVWWLEGFAEYMHYKQGYQAAIELIAQGKMSLSQVFATSYSHDTNRIYRWGYLAVRFMLENHPQEVEGLLALSRSGQFEQWAQQVQTLGQQYDGEFARWLDGLEVTPENPDTDPDTPTEPSDGVTQLQANQSITLSGKAYSEKLFYVDVPANTTHFSVAIEGDGDADLYMSYNQVAHYYDFEVSKFVDGSNEEIQFAADASGYVKPGRYYLSVTGRGRYQAVNLTATIDTAAPTPPTQEQDDLAPVMLQSGQAQHLTVHQQRYAAVYVPEGVSEVRIWLSDLTSSDSQGNVNLYASREHWPTPEQHQFASRYAGSNQYLAIPVEQAGYLHFSLNAPQQGDDVEMVVYFH |
Enzyme Length | 807 |
Uniprot Accession Number | Q8D4Y9 |
Absorption | |
Active Site | ACT_SITE 435; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Digestion of native collagen in the triple helical region at Xaa-|-Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1', Pro and Ala at P2 and P2', and hydroxyproline, Ala or Arg at P3'.; EC=3.4.24.3; |
DNA Binding | |
EC Number | 3.4.24.3 |
Enzyme Function | FUNCTION: Possesses gelatinolytic activity. {ECO:0000250}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (1); Chain (1); Metal binding (2); Region (1); Signal peptide (1) |
Keywords | Collagen degradation;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Signal;Zinc;Zymogen |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..27; /evidence=ECO:0000255 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 90,009 |
Kinetics | |
Metal Binding | METAL 434; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 438; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
Rhea ID | |
Cross Reference Brenda |