| IED ID | IndEnz0002016793 |
| Enzyme Type ID | protease016793 |
| Protein Name |
Aspartic proteinase 36 EC 3.4.23.- |
| Gene Name | A36 At5g36260 T30G6.12 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MVTTMDPSRISRIVAVVFVLVIQVVSGNFVFNVTHKFAGKEKQLSELKSHDSFRHARMLANIDLPLGGDSRADSIGLYFTKIKLGSPPKEYYVQVDTGSDILWVNCAPCPKCPVKTDLGIPLSLYDSKTSSTSKNVGCEDDFCSFIMQSETCGAKKPCSYHVVYGDGSTSDGDFIKDNITLEQVTGNLRTAPLAQEVVFGCGKNQSGQLGQTDSAVDGIMGFGQSNTSIISQLAAGGSTKRIFSHCLDNMNGGGIFAVGEVESPVVKTTPIVPNQVHYNVILKGMDVDGDPIDLPPSLASTNGDGGTIIDSGTTLAYLPQNLYNSLIEKITAKQQVKLHMVQETFACFSFTSNTDKAFPVVNLHFEDSLKLSVYPHDYLFSLREDMYCFGWQSGGMTTQDGADVILLGDLVLSNKLVVYDLENEVIGWADHNCSSSIKVKDGSGAAYQLGAENLISAASSVMNGTLVTLLSILIWVFHSFTS |
| Enzyme Length | 482 |
| Uniprot Accession Number | Q4V3D2 |
| Absorption | |
| Active Site | ACT_SITE 96; /evidence=ECO:0000255|PROSITE-ProRule:PRU01103; ACT_SITE 310; /evidence=ECO:0000255|PROSITE-ProRule:PRU01103 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.23.- |
| Enzyme Function | FUNCTION: Displays aspartic proteolytic activity (PubMed:27872247). Together with A39, contributes to pollen and ovule development, including the apical cell wall constitution of the growing pollen tubes (PubMed:27872247). {ECO:0000269|PubMed:27872247}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (1); Domain (1); Erroneous initiation (1); Glycosylation (6); Lipidation (1); Mutagenesis (2); Propeptide (1); Signal peptide (1) |
| Keywords | Aspartyl protease;Cell membrane;Cytoplasm;Disulfide bond;GPI-anchor;Glycoprotein;Hydrolase;Lipoprotein;Membrane;Protease;Reference proteome;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:27872247}; Lipid-anchor, GPI-anchor {ECO:0000255}. Cytoplasm, cytosol {ECO:0000269|PubMed:27872247}. Note=Displays punctate cytosolic localization and colocalizes with the GPI-anchored protein COBL10 at the plasma membrane and in the cytosol (PubMed:27872247). In pollen grains, mainly present at the plasma membrane and in reticular structures in the cytosol (PubMed:27872247). In pollen tubes, exhibits a punctate localization in the cytosol and occurs weakly at the plasma membrane (PubMed:27872247). {ECO:0000269|PubMed:27872247}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..27; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12068095; 12805588; 14671020; 14697270; 18775970; |
| Motif | |
| Gene Encoded By | |
| Mass | 51,981 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |