Detail Information for IndEnz0002016804
IED ID IndEnz0002016804
Enzyme Type ID protease016804
Protein Name Chaperone protein ClpB1
ATP-dependent Clp protease ATP-binding subunit ClpB homolog 1
Casein lytic proteinase B1
Heat shock protein 101
Protein DEFECTIVE IN LONG-TERM ACQUIRED THERMOTOLERANCE
Gene Name CLPB1 DLT1 HOT1 HSP101 At1g74310 F1O17.2
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MNPEKFTHKTNETIATAHELAVNAGHAQFTPLHLAGALISDPTGIFPQAISSAGGENAAQSAERVINQALKKLPSQSPPPDDIPASSSLIKVIRRAQAAQKSRGDTHLAVDQLIMGLLEDSQIRDLLNEVGVATARVKSEVEKLRGKEGKKVESASGDTNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAVVEGLAQRIVKGDVPNSLTDVRLISLDMGALVAGAKYRGEFEERLKSVLKEVEDAEGKVILFIDEIHLVLGAGKTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFERRFQQVYVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALINAAQLSARYITGRHLPDKAIDLVDEACANVRVQLDSQPEEIDNLERKRMQLEIELHALEREKDKASKARLIEVRKELDDLRDKLQPLTMKYRKEKERIDEIRRLKQKREELMFSLQEAERRYDLARAADLRYGAIQEVESAIAQLEGTSSEENVMLTENVGPEHIAEVVSRWTGIPVTRLGQNEKERLIGLADRLHKRVVGQNQAVNAVSEAILRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQLFDDENLLVRIDMSEYMEQHSVSRLIGAPPGYVGHEEGGQLTEAVRRRPYCVILFDEVEKAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNSVIIMTSNLGAEHLLAGLTGKVTMEVARDCVMREVRKHFRPELLNRLDEIVVFDPLSHDQLRKVARLQMKDVAVRLAERGVALAVTDAALDYILAESYDPVYGARPIRRWMEKKVVTELSKMVVREEIDENSTVYIDAGAGDLVYRVESGGLVDASTGKKSDVLIHIANGPKRSDAAQAVKKMRIEEIEDDDNEEMIED
Enzyme Length 911
Uniprot Accession Number P42730
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Molecular chaperone that plays an important role in thermotolerance. Together with HSA32, required for long-term acquired thermotolerance (LAT) in plants and naturally high basal thermotolerance observed in germinating seedlings. {ECO:0000269|PubMed:10760238, ECO:0000269|PubMed:10760305, ECO:0000269|PubMed:11489180, ECO:0000269|PubMed:15659638, ECO:0000269|PubMed:17144892, ECO:0000269|PubMed:23439916, ECO:0000269|PubMed:7866032}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding NP_BIND 207..214; /note=ATP; /evidence=ECO:0000255; NP_BIND 606..613; /note=ATP; /evidence=ECO:0000255
Features Chain (1); Coiled coil (1); Domain (1); Mutagenesis (7); Nucleotide binding (2); Region (4); Sequence conflict (2)
Keywords ATP-binding;Chaperone;Coiled coil;Cytoplasm;Nucleotide-binding;Nucleus;Reference proteome;Repeat;Stress response
Interact With
Induction INDUCTION: By heat stress and oilseed rape mosaic virus (PubMed:10760238, PubMed:10760305, PubMed:11489180, PubMed:16644052, PubMed:16995899, PubMed:17144892, PubMed:7866032). Regulated by HSA32 that retards its decay in a positive feedback loop (at protein level) during recovery after heat treatment (PubMed:23439916). {ECO:0000269|PubMed:10760238, ECO:0000269|PubMed:10760305, ECO:0000269|PubMed:11489180, ECO:0000269|PubMed:16644052, ECO:0000269|PubMed:16995899, ECO:0000269|PubMed:17144892, ECO:0000269|PubMed:23439916, ECO:0000269|PubMed:7866032}.
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm, cytosol. Nucleus {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 10760235; 12650620; 12678556; 12805605; 15086804; 15763661; 15923322; 16972866; 17059406; 17059409; 17220197; 17238907; 17556511; 17631526; 17883376; 18047473; 18321256; 18641404; 18775970; 19125253; 19452453; 20229063; 20811767; 21835493; 22942382; 22995285; 23080295; 23238922; 23258370; 23893171; 24769482; 25281707; 25711624; 25849955; 25986972; 26286225; 27474115; 27673599; 27811081; 28381501; 28705956; 29449855; 31113833; 31309322; 31519798; 32010424; 32573848; 32595686; 32929162;
Motif
Gene Encoded By
Mass 101,295
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda