| IED ID | IndEnz0002016804 |
| Enzyme Type ID | protease016804 |
| Protein Name |
Chaperone protein ClpB1 ATP-dependent Clp protease ATP-binding subunit ClpB homolog 1 Casein lytic proteinase B1 Heat shock protein 101 Protein DEFECTIVE IN LONG-TERM ACQUIRED THERMOTOLERANCE |
| Gene Name | CLPB1 DLT1 HOT1 HSP101 At1g74310 F1O17.2 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MNPEKFTHKTNETIATAHELAVNAGHAQFTPLHLAGALISDPTGIFPQAISSAGGENAAQSAERVINQALKKLPSQSPPPDDIPASSSLIKVIRRAQAAQKSRGDTHLAVDQLIMGLLEDSQIRDLLNEVGVATARVKSEVEKLRGKEGKKVESASGDTNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAVVEGLAQRIVKGDVPNSLTDVRLISLDMGALVAGAKYRGEFEERLKSVLKEVEDAEGKVILFIDEIHLVLGAGKTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFERRFQQVYVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALINAAQLSARYITGRHLPDKAIDLVDEACANVRVQLDSQPEEIDNLERKRMQLEIELHALEREKDKASKARLIEVRKELDDLRDKLQPLTMKYRKEKERIDEIRRLKQKREELMFSLQEAERRYDLARAADLRYGAIQEVESAIAQLEGTSSEENVMLTENVGPEHIAEVVSRWTGIPVTRLGQNEKERLIGLADRLHKRVVGQNQAVNAVSEAILRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQLFDDENLLVRIDMSEYMEQHSVSRLIGAPPGYVGHEEGGQLTEAVRRRPYCVILFDEVEKAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNSVIIMTSNLGAEHLLAGLTGKVTMEVARDCVMREVRKHFRPELLNRLDEIVVFDPLSHDQLRKVARLQMKDVAVRLAERGVALAVTDAALDYILAESYDPVYGARPIRRWMEKKVVTELSKMVVREEIDENSTVYIDAGAGDLVYRVESGGLVDASTGKKSDVLIHIANGPKRSDAAQAVKKMRIEEIEDDDNEEMIED |
| Enzyme Length | 911 |
| Uniprot Accession Number | P42730 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Molecular chaperone that plays an important role in thermotolerance. Together with HSA32, required for long-term acquired thermotolerance (LAT) in plants and naturally high basal thermotolerance observed in germinating seedlings. {ECO:0000269|PubMed:10760238, ECO:0000269|PubMed:10760305, ECO:0000269|PubMed:11489180, ECO:0000269|PubMed:15659638, ECO:0000269|PubMed:17144892, ECO:0000269|PubMed:23439916, ECO:0000269|PubMed:7866032}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 207..214; /note=ATP; /evidence=ECO:0000255; NP_BIND 606..613; /note=ATP; /evidence=ECO:0000255 |
| Features | Chain (1); Coiled coil (1); Domain (1); Mutagenesis (7); Nucleotide binding (2); Region (4); Sequence conflict (2) |
| Keywords | ATP-binding;Chaperone;Coiled coil;Cytoplasm;Nucleotide-binding;Nucleus;Reference proteome;Repeat;Stress response |
| Interact With | |
| Induction | INDUCTION: By heat stress and oilseed rape mosaic virus (PubMed:10760238, PubMed:10760305, PubMed:11489180, PubMed:16644052, PubMed:16995899, PubMed:17144892, PubMed:7866032). Regulated by HSA32 that retards its decay in a positive feedback loop (at protein level) during recovery after heat treatment (PubMed:23439916). {ECO:0000269|PubMed:10760238, ECO:0000269|PubMed:10760305, ECO:0000269|PubMed:11489180, ECO:0000269|PubMed:16644052, ECO:0000269|PubMed:16995899, ECO:0000269|PubMed:17144892, ECO:0000269|PubMed:23439916, ECO:0000269|PubMed:7866032}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm, cytosol. Nucleus {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10760235; 12650620; 12678556; 12805605; 15086804; 15763661; 15923322; 16972866; 17059406; 17059409; 17220197; 17238907; 17556511; 17631526; 17883376; 18047473; 18321256; 18641404; 18775970; 19125253; 19452453; 20229063; 20811767; 21835493; 22942382; 22995285; 23080295; 23238922; 23258370; 23893171; 24769482; 25281707; 25711624; 25849955; 25986972; 26286225; 27474115; 27673599; 27811081; 28381501; 28705956; 29449855; 31113833; 31309322; 31519798; 32010424; 32573848; 32595686; 32929162; |
| Motif | |
| Gene Encoded By | |
| Mass | 101,295 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |