Detail Information for IndEnz0002016905
IED ID IndEnz0002016905
Enzyme Type ID protease016905
Protein Name Chaperone protein ClpC1, chloroplastic
ATP-dependent Clp protease ATP-binding subunit ClpC homolog 1
Casein lytic proteinase C1
Protein DE-REGULATED CAO ACCUMULATION 1
Protein IRON-RESCUED MUTANT 1
Gene Name CLPC1 DCA1 HSP93-V IRM1 At5g50920 K3K7.7
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MAMATRVLAQSTPPSLACYQRNVPSRGSGRSRRSVKMMCSQLQVSGLRMQGFMGLRGNNALDTLGKSRQDFHSKVRQAMNVPKGKASRFTVKAMFERFTEKAIKVIMLAQEEARRLGHNFVGTEQILLGLIGEGTGIAAKVLKSMGINLKDARVEVEKIIGRGSGFVAVEIPFTPRAKRVLELSLEEARQLGHNYIGSEHLLLGLLREGEGVAARVLENLGADPSNIRTQVIRMVGENNEVTANVGGGSSSNKMPTLEEYGTNLTKLAEEGKLDPVVGRQPQIERVVQILGRRTKNNPCLIGEPGVGKTAIAEGLAQRIASGDVPETIEGKKVITLDMGLLVAGTKYRGEFEERLKKLMEEIRQSDEIILFIDEVHTLIGAGAAEGAIDAANILKPALARGELQCIGATTLDEYRKHIEKDPALERRFQPVKVPEPTVDETIQILKGLRERYEIHHKLRYTDESLVAAAQLSYQYISDRFLPDKAIDLIDEAGSRVRLRHAQVPEEARELEKELRQITKEKNEAVRGQDFEKAGTLRDREIELRAEVSAIQAKGKEMSKAESETGEEGPMVTESDIQHIVSSWTGIPVEKVSTDESDRLLKMEETLHKRIIGQDEAVKAISRAIRRARVGLKNPNRPIASFIFSGPTGVGKSELAKALAAYYFGSEEAMIRLDMSEFMERHTVSKLIGSPPGYVGYTEGGQLTEAVRRRPYTVVLFDEIEKAHPDVFNMMLQILEDGRLTDSKGRTVDFKNTLLIMTSNVGSSVIEKGGRRIGFDLDYDEKDSSYNRIKSLVTEELKQYFRPEFLNRLDEMIVFRQLTKLEVKEIADILLKEVFERLKKKEIELQVTERFKERVVDEGYNPSYGARPLRRAIMRLLEDSMAEKMLAREIKEGDSVIVDVDAEGNVTVLNGGSGTPTTSLEEQEDSLPVA
Enzyme Length 929
Uniprot Accession Number Q9FI56
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Molecular chaperone that hydrolyzes ATP and is associated with the chloroplast protein import apparatus. May function as the motor for chloroplast protein translocation, as translocation requires ATP hydrolysis in the stroma. May interact with a ClpP-like protease involved in degradation of denatured proteins in the chloroplast. Involved in the regulation of chlorophyll b biosynthesis through the destabilization of chlorophyllide a oxygenase (CAO) protein in response to the accumulation of chlorophyll b. Involved in leaf iron homeostasis. {ECO:0000269|PubMed:15304652, ECO:0000269|PubMed:15516497, ECO:0000269|PubMed:15659100, ECO:0000269|PubMed:17291312, ECO:0000269|PubMed:17376159, ECO:0000269|PubMed:20382967, ECO:0000269|PubMed:22353577, ECO:0000269|PubMed:24599948}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding NP_BIND 302..309; /note=ATP; /evidence=ECO:0000255; NP_BIND 645..652; /note=ATP; /evidence=ECO:0000255
Features Beta strand (1); Chain (1); Compositional bias (1); Domain (2); Helix (8); Mutagenesis (1); Nucleotide binding (2); Region (6); Sequence conflict (5); Transit peptide (1)
Keywords 3D-structure;ATP-binding;Chaperone;Chloroplast;Membrane;Nucleotide-binding;Plastid;Protein transport;Reference proteome;Repeat;Transit peptide;Transport
Interact With Q8LPR9; Q9FMD5
Induction INDUCTION: By cold and salt stresses. Not induced by heat stress. {ECO:0000269|PubMed:11982939, ECO:0000269|PubMed:17144892}.
Subcellular Location SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000269|PubMed:11982939, ECO:0000269|PubMed:14593120, ECO:0000269|PubMed:20382967, ECO:0000269|PubMed:24599948}. Plastid, chloroplast membrane {ECO:0000269|PubMed:24599948}. Note=The membrane association is important for the in vivo functions. {ECO:0000269|PubMed:24599948}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (1)
Cross Reference PDB 5GUI;
Mapped Pubmed ID 10198079; 10427773; 11299338; 12766230; 12938931; 14671022; 15028209; 15322131; 16207701; 16287169; 17158958; 18324730; 18431481; 18633119; 19086173; 19130088; 20118269; 20484004; 21166475; 21737456; 22995300; 24064926; 26586836; 26815787; 26884485; 27170143; 28287170; 28627464; 28937985; 29155321; 30032295; 30208840; 30927692; 31070379; 32344669; 7580259; 9405363; 9951729;
Motif
Gene Encoded By
Mass 103,453
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda 7.4.2.4;