| IED ID | IndEnz0002016905 |
| Enzyme Type ID | protease016905 |
| Protein Name |
Chaperone protein ClpC1, chloroplastic ATP-dependent Clp protease ATP-binding subunit ClpC homolog 1 Casein lytic proteinase C1 Protein DE-REGULATED CAO ACCUMULATION 1 Protein IRON-RESCUED MUTANT 1 |
| Gene Name | CLPC1 DCA1 HSP93-V IRM1 At5g50920 K3K7.7 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAMATRVLAQSTPPSLACYQRNVPSRGSGRSRRSVKMMCSQLQVSGLRMQGFMGLRGNNALDTLGKSRQDFHSKVRQAMNVPKGKASRFTVKAMFERFTEKAIKVIMLAQEEARRLGHNFVGTEQILLGLIGEGTGIAAKVLKSMGINLKDARVEVEKIIGRGSGFVAVEIPFTPRAKRVLELSLEEARQLGHNYIGSEHLLLGLLREGEGVAARVLENLGADPSNIRTQVIRMVGENNEVTANVGGGSSSNKMPTLEEYGTNLTKLAEEGKLDPVVGRQPQIERVVQILGRRTKNNPCLIGEPGVGKTAIAEGLAQRIASGDVPETIEGKKVITLDMGLLVAGTKYRGEFEERLKKLMEEIRQSDEIILFIDEVHTLIGAGAAEGAIDAANILKPALARGELQCIGATTLDEYRKHIEKDPALERRFQPVKVPEPTVDETIQILKGLRERYEIHHKLRYTDESLVAAAQLSYQYISDRFLPDKAIDLIDEAGSRVRLRHAQVPEEARELEKELRQITKEKNEAVRGQDFEKAGTLRDREIELRAEVSAIQAKGKEMSKAESETGEEGPMVTESDIQHIVSSWTGIPVEKVSTDESDRLLKMEETLHKRIIGQDEAVKAISRAIRRARVGLKNPNRPIASFIFSGPTGVGKSELAKALAAYYFGSEEAMIRLDMSEFMERHTVSKLIGSPPGYVGYTEGGQLTEAVRRRPYTVVLFDEIEKAHPDVFNMMLQILEDGRLTDSKGRTVDFKNTLLIMTSNVGSSVIEKGGRRIGFDLDYDEKDSSYNRIKSLVTEELKQYFRPEFLNRLDEMIVFRQLTKLEVKEIADILLKEVFERLKKKEIELQVTERFKERVVDEGYNPSYGARPLRRAIMRLLEDSMAEKMLAREIKEGDSVIVDVDAEGNVTVLNGGSGTPTTSLEEQEDSLPVA |
| Enzyme Length | 929 |
| Uniprot Accession Number | Q9FI56 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Molecular chaperone that hydrolyzes ATP and is associated with the chloroplast protein import apparatus. May function as the motor for chloroplast protein translocation, as translocation requires ATP hydrolysis in the stroma. May interact with a ClpP-like protease involved in degradation of denatured proteins in the chloroplast. Involved in the regulation of chlorophyll b biosynthesis through the destabilization of chlorophyllide a oxygenase (CAO) protein in response to the accumulation of chlorophyll b. Involved in leaf iron homeostasis. {ECO:0000269|PubMed:15304652, ECO:0000269|PubMed:15516497, ECO:0000269|PubMed:15659100, ECO:0000269|PubMed:17291312, ECO:0000269|PubMed:17376159, ECO:0000269|PubMed:20382967, ECO:0000269|PubMed:22353577, ECO:0000269|PubMed:24599948}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 302..309; /note=ATP; /evidence=ECO:0000255; NP_BIND 645..652; /note=ATP; /evidence=ECO:0000255 |
| Features | Beta strand (1); Chain (1); Compositional bias (1); Domain (2); Helix (8); Mutagenesis (1); Nucleotide binding (2); Region (6); Sequence conflict (5); Transit peptide (1) |
| Keywords | 3D-structure;ATP-binding;Chaperone;Chloroplast;Membrane;Nucleotide-binding;Plastid;Protein transport;Reference proteome;Repeat;Transit peptide;Transport |
| Interact With | Q8LPR9; Q9FMD5 |
| Induction | INDUCTION: By cold and salt stresses. Not induced by heat stress. {ECO:0000269|PubMed:11982939, ECO:0000269|PubMed:17144892}. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000269|PubMed:11982939, ECO:0000269|PubMed:14593120, ECO:0000269|PubMed:20382967, ECO:0000269|PubMed:24599948}. Plastid, chloroplast membrane {ECO:0000269|PubMed:24599948}. Note=The membrane association is important for the in vivo functions. {ECO:0000269|PubMed:24599948}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 5GUI; |
| Mapped Pubmed ID | 10198079; 10427773; 11299338; 12766230; 12938931; 14671022; 15028209; 15322131; 16207701; 16287169; 17158958; 18324730; 18431481; 18633119; 19086173; 19130088; 20118269; 20484004; 21166475; 21737456; 22995300; 24064926; 26586836; 26815787; 26884485; 27170143; 28287170; 28627464; 28937985; 29155321; 30032295; 30208840; 30927692; 31070379; 32344669; 7580259; 9405363; 9951729; |
| Motif | |
| Gene Encoded By | |
| Mass | 103,453 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 7.4.2.4; |