| IED ID | IndEnz0002016922 |
| Enzyme Type ID | protease016922 |
| Protein Name |
Chaperone protein ClpC2, chloroplastic EC 3.6.1.- ATP-dependent Clp protease ATP-binding subunit ClpC homolog 2 AtClpC Casein lytic proteinase C2 |
| Gene Name | CLPC2 HSP93-III At3g48870 T21J18.140 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAWSIALLTPPFFGPGRHVQAKEYREPRGCVMKMSSLKAPVLRIQATEYREPRGRVKMMSSLQAPLLTIQSFSGLRAPSALDYLGRPSPGFLVKYKLAKSSGREKASRCVPKAMFERFTEKAIKVIMLSQEEARRLGHNFVGTEQILLGLIGEGTGIAAKVLKSMGINLKDSRVEVEKIIGRGSGFVAVEIPFTPRAKRVLELSLEEARQLGHNYIGSEHLLLGLLREGEGVAARVLENLGADPSNIRTQVIRMVGENNEVTASVGGGSSGNSKMPTLEEYGTNLTKLAEEGKLDPVVGRQPQIERVVQILARRTKNNPCLIGEPGVGKTAIAEGLAQRIASGDVPETIEGKTVITLDMGLLVAGTKYRGEFEERLKKLMEEIRQSDEIILFIDEVHTLIGAGAAEGAIDAANILKPALARGELQCIGATTIDEYRKHIEKDPALERRFQPVKVPEPTVEEAIQILQGLRERYEIHHKLRYTDEALVAAAQLSHQYISDRFLPDKAIDLIDEAGSRVRLRHAQLPEEARELEKQLRQITKEKNEAVRSQDFEMAGSHRDREIELKAEIANVLSRGKEVAKAENEAEEGGPTVTESDIQHIVATWTGIPVEKVSSDESSRLLQMEQTLHTRVIGQDEAVKAISRAIRRARVGLKNPNRPIASFIFSGPTGVGKSELAKALAAYYFGSEEAMIRLDMSEFMERHTVSKLIGSPPGYVGYTEGGQLTEAVRRRPYTLVLFDEIEKAHPDVFNMMLQILEDGRLTDSKGRTVDFKNTLLIMTSNVGSSVIEKGGRRIGFDLDHDEKDSSYNRIKSLVTEELKQYFRPEFLNRLDEMIVFRQLTKLEVKEIADIMLKEVVARLEVKEIELQVTERFKERVVDEGFDPSYGARPLRRAIMRLLEDSMAEKMLSRDIKEGDSVIVDVDAEGSVVVLSGTTGRVGGFAAEEAMEDPIPIL |
| Enzyme Length | 952 |
| Uniprot Accession Number | Q9SXJ7 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; Evidence={ECO:0000269|PubMed:21737456}; |
| DNA Binding | |
| EC Number | 3.6.1.- |
| Enzyme Function | FUNCTION: Molecular chaperone (PubMed:15304652, PubMed:21737456, PubMed:24599948). May act as a suppressor of FtsH-mediated thylakoid membrane biogenesis and may enhance photoinhibition (PubMed:15304652). Seems not involved in chloroplastic protein import (PubMed:15304652). Probable component of the TIC-associated stromal import motor involved in inner membrane translocation (PubMed:17376159). Has an ATPase activity, but no ADPase activity (PubMed:21737456). Interacts with transit peptides with a positional preference (PubMed:21737456, PubMed:22545953). Localization of the signal sequence at the N-terminal end of a protein seems mandatory for interaction to take place (PubMed:22545953). {ECO:0000269|PubMed:15304652, ECO:0000269|PubMed:17376159, ECO:0000269|PubMed:21737456, ECO:0000269|PubMed:22545953, ECO:0000269|PubMed:24599948}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 55 degrees Celsius. {ECO:0000269|PubMed:21737456}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.5. {ECO:0000269|PubMed:21737456}; |
| Pathway | |
| nucleotide Binding | NP_BIND 323..330; /note=ATP; /evidence=ECO:0000255; NP_BIND 666..673; /note=ATP; /evidence=ECO:0000255 |
| Features | Chain (1); Domain (2); Frameshift (1); Nucleotide binding (2); Region (4); Sequence conflict (2); Transit peptide (1) |
| Keywords | ATP-binding;Alternative splicing;Chaperone;Chloroplast;Hydrolase;Membrane;Nucleotide-binding;Plastid;Reference proteome;Repeat;Transit peptide |
| Interact With | |
| Induction | INDUCTION: By senescence. Not induced by heat stress. {ECO:0000269|PubMed:10427773, ECO:0000269|PubMed:17144892}. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000269|PubMed:10427773, ECO:0000269|PubMed:21737456, ECO:0000269|PubMed:24599948}. Plastid, chloroplast membrane {ECO:0000269|PubMed:21737456, ECO:0000269|PubMed:24599948}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11299338; 12185496; 12938931; 14576160; 14593120; 14671022; 15028209; 15563614; 15829604; 16207701; 16644052; 16766689; 17181860; 17432890; 18431481; 18633119; 19086173; 20118269; 20484004; 22353577; 23395902; 26884485; 29401302; 31070379; |
| Motif | |
| Gene Encoded By | |
| Mass | 105,739 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1.42 mM for ATP {ECO:0000269|PubMed:21737456}; Vmax=0.62 nmol/min/ug enzyme {ECO:0000269|PubMed:21737456}; |
| Metal Binding | |
| Rhea ID | RHEA:13065 |
| Cross Reference Brenda | 7.4.2.4; |