IED ID | IndEnz0002016922 |
Enzyme Type ID | protease016922 |
Protein Name |
Chaperone protein ClpC2, chloroplastic EC 3.6.1.- ATP-dependent Clp protease ATP-binding subunit ClpC homolog 2 AtClpC Casein lytic proteinase C2 |
Gene Name | CLPC2 HSP93-III At3g48870 T21J18.140 |
Organism | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
Enzyme Sequence | MAWSIALLTPPFFGPGRHVQAKEYREPRGCVMKMSSLKAPVLRIQATEYREPRGRVKMMSSLQAPLLTIQSFSGLRAPSALDYLGRPSPGFLVKYKLAKSSGREKASRCVPKAMFERFTEKAIKVIMLSQEEARRLGHNFVGTEQILLGLIGEGTGIAAKVLKSMGINLKDSRVEVEKIIGRGSGFVAVEIPFTPRAKRVLELSLEEARQLGHNYIGSEHLLLGLLREGEGVAARVLENLGADPSNIRTQVIRMVGENNEVTASVGGGSSGNSKMPTLEEYGTNLTKLAEEGKLDPVVGRQPQIERVVQILARRTKNNPCLIGEPGVGKTAIAEGLAQRIASGDVPETIEGKTVITLDMGLLVAGTKYRGEFEERLKKLMEEIRQSDEIILFIDEVHTLIGAGAAEGAIDAANILKPALARGELQCIGATTIDEYRKHIEKDPALERRFQPVKVPEPTVEEAIQILQGLRERYEIHHKLRYTDEALVAAAQLSHQYISDRFLPDKAIDLIDEAGSRVRLRHAQLPEEARELEKQLRQITKEKNEAVRSQDFEMAGSHRDREIELKAEIANVLSRGKEVAKAENEAEEGGPTVTESDIQHIVATWTGIPVEKVSSDESSRLLQMEQTLHTRVIGQDEAVKAISRAIRRARVGLKNPNRPIASFIFSGPTGVGKSELAKALAAYYFGSEEAMIRLDMSEFMERHTVSKLIGSPPGYVGYTEGGQLTEAVRRRPYTLVLFDEIEKAHPDVFNMMLQILEDGRLTDSKGRTVDFKNTLLIMTSNVGSSVIEKGGRRIGFDLDHDEKDSSYNRIKSLVTEELKQYFRPEFLNRLDEMIVFRQLTKLEVKEIADIMLKEVVARLEVKEIELQVTERFKERVVDEGFDPSYGARPLRRAIMRLLEDSMAEKMLSRDIKEGDSVIVDVDAEGSVVVLSGTTGRVGGFAAEEAMEDPIPIL |
Enzyme Length | 952 |
Uniprot Accession Number | Q9SXJ7 |
Absorption | |
Active Site | |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; Evidence={ECO:0000269|PubMed:21737456}; |
DNA Binding | |
EC Number | 3.6.1.- |
Enzyme Function | FUNCTION: Molecular chaperone (PubMed:15304652, PubMed:21737456, PubMed:24599948). May act as a suppressor of FtsH-mediated thylakoid membrane biogenesis and may enhance photoinhibition (PubMed:15304652). Seems not involved in chloroplastic protein import (PubMed:15304652). Probable component of the TIC-associated stromal import motor involved in inner membrane translocation (PubMed:17376159). Has an ATPase activity, but no ADPase activity (PubMed:21737456). Interacts with transit peptides with a positional preference (PubMed:21737456, PubMed:22545953). Localization of the signal sequence at the N-terminal end of a protein seems mandatory for interaction to take place (PubMed:22545953). {ECO:0000269|PubMed:15304652, ECO:0000269|PubMed:17376159, ECO:0000269|PubMed:21737456, ECO:0000269|PubMed:22545953, ECO:0000269|PubMed:24599948}. |
Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 55 degrees Celsius. {ECO:0000269|PubMed:21737456}; |
PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.5. {ECO:0000269|PubMed:21737456}; |
Pathway | |
nucleotide Binding | NP_BIND 323..330; /note=ATP; /evidence=ECO:0000255; NP_BIND 666..673; /note=ATP; /evidence=ECO:0000255 |
Features | Chain (1); Domain (2); Frameshift (1); Nucleotide binding (2); Region (4); Sequence conflict (2); Transit peptide (1) |
Keywords | ATP-binding;Alternative splicing;Chaperone;Chloroplast;Hydrolase;Membrane;Nucleotide-binding;Plastid;Reference proteome;Repeat;Transit peptide |
Interact With | |
Induction | INDUCTION: By senescence. Not induced by heat stress. {ECO:0000269|PubMed:10427773, ECO:0000269|PubMed:17144892}. |
Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000269|PubMed:10427773, ECO:0000269|PubMed:21737456, ECO:0000269|PubMed:24599948}. Plastid, chloroplast membrane {ECO:0000269|PubMed:21737456, ECO:0000269|PubMed:24599948}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | 11299338; 12185496; 12938931; 14576160; 14593120; 14671022; 15028209; 15563614; 15829604; 16207701; 16644052; 16766689; 17181860; 17432890; 18431481; 18633119; 19086173; 20118269; 20484004; 22353577; 23395902; 26884485; 29401302; 31070379; |
Motif | |
Gene Encoded By | |
Mass | 105,739 |
Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1.42 mM for ATP {ECO:0000269|PubMed:21737456}; Vmax=0.62 nmol/min/ug enzyme {ECO:0000269|PubMed:21737456}; |
Metal Binding | |
Rhea ID | RHEA:13065 |
Cross Reference Brenda | 7.4.2.4; |