| IED ID | IndEnz0002016994 |
| Enzyme Type ID | protease016994 |
| Protein Name |
Chaperone protein ClpD, chloroplastic EC 3.6.1.- ATP-dependent Clp protease ATP-binding subunit ClpD homolog Casein lytic proteinase D ERD1 protein Protein EARLY RESPONSIVE TO DEHYDRATION 1 Protein SENESCENCE ASSOCIATED GENE 15 |
| Gene Name | CLPD ERD1 SAG15 At5g51070 K3K7.27 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MEVLSTSSPLTLHSHRLLSASSSSSHVTSIAASSLSSFASSYLGISLSNRTIHRFSTTPTNLRRFPQRKRKKFTPISAVFERFTERAIRAIIFSQKEAKSLGKDMVYTQHLLLGLIAEDRDPQGFLGSGITIDKAREAVWSIWDEANSDSKQEEASSTSYSKSTDMPFSISTKRVFEAAVEYSRTMDCQYIAPEHIAVGLFTVDDGSAGRVLKRLGANMNLLTAAALTRLKGEIAKDGREPSSSSKGSFESPPSGRIAGSGPGGKKAKNVLEQFCVDLTARASEGLIDPVIGREKEVQRVIQILCRRTKNNPILLGEAGVGKTAIAEGLAISIAEASAPGFLLTKRIMSLDIGLLMAGAKERGELEARVTALISEVKKSGKVILFIDEVHTLIGSGTVGRGNKGSGLDIANLLKPSLGRGELQCIASTTLDEFRSQFEKDKALARRFQPVLINEPSEEDAVKILLGLREKYEAHHNCKYTMEAIDAAVYLSSRYIADRFLPDKAIDLIDEAGSRARIEAFRKKKEDAICILSKPPNDYWQEIKTVQAMHEVVLSSRQKQDDGDAISDESGELVEESSLPPAAGDDEPILVGPDDIAAVASVWSGIPVQQITADERMLLMSLEDQLRGRVVGQDEAVAAISRAVKRSRVGLKDPDRPIAAMLFCGPTGVGKTELTKALAANYFGSEESMLRLDMSEYMERHTVSKLIGSPPGYVGFEEGGMLTEAIRRRPFTVVLFDEIEKAHPDIFNILLQLFEDGHLTDSQGRRVSFKNALIIMTSNVGSLAIAKGRHGSIGFILDDDEEAASYTGMKALVVEELKNYFRPELLNRIDEIVIFRQLEKAQMMEILNLMLQDLKSRLVALGVGLEVSEPVKELICKQGYDPAYGARPLRRTVTEIVEDPLSEAFLAGSFKPGDTAFVVLDDTGNPSVRTKPDSSTIRVTDKTSIA |
| Enzyme Length | 945 |
| Uniprot Accession Number | P42762 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; Evidence={ECO:0000269|PubMed:21737456}; |
| DNA Binding | |
| EC Number | 3.6.1.- |
| Enzyme Function | FUNCTION: Molecular chaperone that interact with a ClpP-like protease involved in degradation of denatured proteins in the chloroplast (PubMed:21737456). The ATPase activity of CLPD is stimulated by CLPT1 (PubMed:25149061). Has no ADPase activity (PubMed:21737456). Interacts with transit peptides with a positional preference (PubMed:21737456, PubMed:22545953). Localization of the signal sequence at the N-terminal end of a protein seems mandatory for interaction to take place (PubMed:22545953). {ECO:0000269|PubMed:21737456, ECO:0000269|PubMed:22545953, ECO:0000269|PubMed:25149061}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 45 degrees Celsius. {ECO:0000269|PubMed:21737456}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.5. {ECO:0000269|PubMed:21737456}; |
| Pathway | |
| nucleotide Binding | NP_BIND 316..323; /note=ATP; /evidence=ECO:0000250; NP_BIND 664..671; /note=ATP; /evidence=ECO:0000250 |
| Features | Chain (1); Domain (1); Nucleotide binding (2); Region (6); Transit peptide (1) |
| Keywords | ATP-binding;Chaperone;Chloroplast;Hydrolase;Nucleotide-binding;Plastid;Reference proteome;Repeat;Stress response;Transit peptide |
| Interact With | |
| Induction | INDUCTION: By dehydration and salt stresses. Induced after one hour of dehydration-stress and reaches maximal levels after 10 hours. Induced by cold, ozone, senescence and dark-induced etiolation. Down-regulated by ozone (at protein level). Not induced by heat stress. {ECO:0000269|PubMed:10198079, ECO:0000269|PubMed:10427773, ECO:0000269|PubMed:10444084, ECO:0000269|PubMed:11982939, ECO:0000269|PubMed:17144892, ECO:0000269|PubMed:9375397}. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000269|PubMed:10198079, ECO:0000269|PubMed:10427773, ECO:0000269|PubMed:11982939, ECO:0000269|PubMed:21737456, ECO:0000269|PubMed:9375397}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10189710; 12054542; 12535340; 12938931; 15319476; 16998088; 17233795; 17259181; 18431481; 18650403; 18705666; 24599948; 26419670; 27247031; 28287170; 29155321; 31070379; 8075396; 9847097; |
| Motif | |
| Gene Encoded By | |
| Mass | 103,235 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=19.8 mM for ATP {ECO:0000269|PubMed:21737456}; Vmax=0.19 nmol/min/ug enzyme {ECO:0000269|PubMed:21737456}; |
| Metal Binding | |
| Rhea ID | RHEA:13065 |
| Cross Reference Brenda |